Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus
| العنوان: | Evaluation of Anti-Mycobacterial Compounds in a Silkworm Infection Model with Mycobacteroides abscessus |
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| المؤلفون: | Akiho Yagi, Hiroshi Tomoda, Nobuhiro Koyama, Kanji Hosoda, Hiroshi Hamamoto, Akihiko Kanamoto, Ryuji Uchida |
| المصدر: | Molecules, Vol 25, Iss 4971, p 4971 (2020) Molecules Volume 25 Issue 21 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | nontuberculous mycobacteria, ohmyungsamycin, animal structures, medicine.drug_class, mycobacteria, Antibiotics, nosiheptide, quinomycin, Pharmaceutical Science, Analytical Chemistry, Microbiology, lcsh:QD241-441, 03 medical and health sciences, chemistry.chemical_compound, lcsh:Organic chemistry, Clarithromycin, Drug Discovery, Hemolymph, medicine, Physical and Theoretical Chemistry, steffimycin, silkworm, Mycobacterium avium complex, 030304 developmental biology, 0303 health sciences, antimycobacterial activity, biology, 030306 microbiology, microbial product, Organic Chemistry, fungi, lariatin, biology.organism_classification, Antimicrobial, bacterial infections and mycoses, chemistry, Chemistry (miscellaneous), Amikacin, Mycobacteroides abscessus, Molecular Medicine, bacteria, Nontuberculous mycobacteria, Nosiheptide, medicine.drug, Mycobacterium |
| الوصف: | Among four mycobacteria, Mycobacterium avium, M. intracellulare, M. bovis BCG and Mycobacteroides (My.) abscessus, we established a silkworm infection assay with My. abscessus. When silkworms (fifth-instar larvae, n = 5) were infected through the hemolymph with My. abscessus (7.5 × 107 CFU/larva) and bred at 37 ° C, they all died around 40 h after injection. Under the conditions, clarithromycin and amikacin, clinically used antimicrobial agents, exhibited therapeutic effects in a dose-dependent manner. Furthermore, five kinds of microbial compounds, lariatin A, nosiheptide, ohmyungsamycins A and B, quinomycin and steffimycin, screened in an in vitro assay to observe anti-My. abscessus activity from 400 microbial products were evaluated in this silkworm infection assay. Lariatin A and nosiheptide exhibited therapeutic efficacy. The silkworm infection model with My. abscessus is useful to screen for therapeutically effective anti-My. abscessus antibiotics. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1420-3049 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b84eb7106d5df44141ccb98add0c7c8Test https://www.mdpi.com/1420-3049/25/21/4971Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3b84eb7106d5df44141ccb98add0c7c8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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