نتائج البحث - "Worker bee"

1

Possibility to change the body size in worker bees by a combination of small-cell and standard-cell combs in the same nest

المؤلفون: Krzysztof Olszewski, Grzegorz Borsuk, Piotr Dziechciarz

المصدر: Apidologie. 52:1017-1032

مصطلحات موضوعية: animal structures, Wing, biology, fungi, Body size, Width ratio, biology.organism_classification, Body weight, Worker bee, Animal science, Nest, Insect Science, Varroa destructor, Thorax (insect anatomy)

الوصف: The aim of the study was to investigate the impact of the combination of the colony type (kept on small-cell or standard-cell combs) and the width of worker comb cells (small-cell or standard-cell combs) on the body weight and morphometric traits of worker bees. The values of morphometric parameters of worker bees changed within a substantially lower range than the width of their rearing cells. This indicates that the worker body size is relatively constant, and manipulation with the cell width is not a good method for modeling the body size of workers. The reduction in the thorax weight was proportional to the decrease in the comb cell width, and this part of the body proved to be most susceptible to weight reduction caused by the use of small-cell combs. The rearing of workers in small-cell combs in the colony kept on standard-cell combs resulted in an increase in the value of the fill factor (thorax width to cell width ratio). The relatively constant body size of workers in combination with the use of small-cell combs resulting in an increase in the fill factor may be one of the determinants of increased resistance of the insects to Varroa destructor. The values of the morphometric traits commonly used for identification of honeybee subspecies, i.e., the length of the fore wing, the sum of the widths of 3rd and 4thth tergites, and the proboscis length, were inconsiderably altered vs. the changes in the comb cell width, which confirms their high suitability for identification of honeybee subspecies.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::05d6023d4f543e886f0b56ed883a2e23Test
https://doi.org/10.1007/s13592-021-00884-4Test

2

Comparison of eggs produced by queens, halfqueens and worker bees and weight of emerging workers in Apis mellifera lamarckii

المؤلفون: Ahmed Saad Abou Zeid

المصدر: Journal of Apicultural Research. 59:685-690

مصطلحات موضوعية: 0106 biological sciences, animal structures, fungi, food and beverages, Zoology, 04 agricultural and veterinary sciences, Honey bee, Biology, 040401 food science, 01 natural sciences, Worker bee, 010602 entomology, 0404 agricultural biotechnology, Insect Science, behavior and behavior mechanisms, reproductive and urinary physiology

الوصف: This work aimed to compare the measurements, developmental length, and weight of eggs laid by colony queens naturally mated halfqueens and laying workers of the Egyptian honey bee Apis mellifera la...

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::cea0e001f49441c891a29533d894cdb1Test
https://doi.org/10.1080/00218839.2020.1770419Test

3

Neutral sterols in honey bee (Apis mellifera) feces

المؤلفون: Dawn J. Harrison, Mark F. Feldlaufer

المصدر: Journal of Apicultural Research. 59:1033-1036

مصطلحات موضوعية: animal structures, fungi, food and beverages, Honey bee, Biology, medicine.disease_cause, Sterol, Worker bee, Insect Science, Pollen, behavior and behavior mechanisms, medicine, lipids (amino acids, peptides, and proteins), Food science, Plant sterols, Feces

الوصف: Nutrition is important in honey bee (Apis mellifera L.) colony decline. While the honey bee is incapable of converting dietary plant sterols to cholesterol, the sterol 24-methylenecholesterol has b...

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::ff75e83e571bccbf18c99bfedcd988deTest
https://doi.org/10.1080/00218839.2020.1753917Test

4

Detection and quantification of Melissococcus plutonius in honey bee workers exposed to European foulbrood in Czechia through conventional PCR, qPCR, and barcode sequencing

المؤلفون: Tomas Erban, Martin Markovic, Martin Kamler, Jan Hubert, Marta Nesvorna, Justyna Zitek, Dalibor Titera, Bruno Sopko

المصدر: Journal of Apicultural Research. 59:503-514

مصطلحات موضوعية: Worker bee, animal structures, Bacterial disease, Insect Science, fungi, behavior and behavior mechanisms, food and beverages, Honey bee, Microbiome, Biology, Melissococcus plutonius, complex mixtures, Microbiology

الوصف: Melissococcus plutonius is the causative agent of European foulbrood (EFB), an important bacterial disease of honey bee larvae, and various methods have been developed for the detection of this dis...

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::60acd0bede3312113ddb94beaf5a8be4Test
https://doi.org/10.1080/00218839.2019.1685148Test

5

Genome-Wide Differential DNA Methylation in Reproductive, Morphological, and Visual System Differences Between Queen Bee and Worker Bee (Apis mellifera)

المؤلفون: Ying Wang, Zhenguo Liu, Weixing Zhang, Lanting Ma, Baohua Xu, Hongfang Wang

المصدر: Frontiers in Genetics
Frontiers in Genetics, Vol 11 (2020)

الوصف: There are many differences in external morphology and internal physiology between the Apis mellifera queen bee and worker bee, some of which are relevant to beekeeping production. These include reproductive traits, body size, royal jelly secreting properties, and visual system development, among others. The identification of candidate genes that control the differentiation of these traits is critical for selective honeybee breeding programs. In this study, we compared the genomic methylation of queen bee and worker bee larvae at 3, 4, and 5 days of age by whole-genome bisulfite sequencing, and found that the basic characteristics of genomic methylation in queen and worker larvae were the same. There were approximately 49 million cytosines in the Apis larvae genome, of which about 90,000 were methylated. Methylated CpG sites accounted for 99% of the methylated cytosines, and methylation mainly occurred in exons. However, methylation levels of queen and worker larvae showed different trends with age: the methylation level of queen larvae varied with age in an inverted parabola, while the corresponding trend for worker larvae with resembled an exponential curve with a platform. The methylation level of queen larvae was higher than that of worker larvae at 3 days of age, lower than that of worker larvae at 4 days of age, and similar to that of worker larvae at 5 days old. The top 10 differentially methylated genes (DMGs) and 13 caste-specific methylated genes were listed, and correlations with caste determination were speculated. We additionally screened 38 DMGs between queen larvae and worker larvae involved in specific organ differentiation as well as reproduction, morphology, and vision differentiation during caste determination. These genes are potential molecular markers for selective breeding of A. mellifera to improve fecundity, royal jelly production, body size, and foraging, and represent candidate genes for investigating specialized functional segregation during the process of caste differentiation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f05dcb98fbdb875669ac0af4ba087a6Test
https://doi.org/10.3389/fgene.2020.00770Test

6

Pupal cannibalism by worker honey bees contributes to the spread of deformed wing virus

المؤلفون: Francisco Posada-Florez, David J. Hawthorne, Jay D. Evans, Yanping Chen, Zachary S. Lamas, Eugene V. Ryabov

المصدر: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)

الوصف: Transmission routes impact pathogen virulence and genetics, therefore comprehensive knowledge of these routes and their contribution to pathogen circulation is essential for understanding host-pathogen interactions and designing control strategies. Deformed wing virus (DWV), a principal viral pathogen of honey bees associated with increased honey bee mortality and colony losses, became highly virulent with the spread of its vector, the ectoparasitic mite Varroa destructor. Reproduction of Varroa mites occurs in capped brood cells and mite-infested pupae from these cells usually have high levels of DWV. The removal of mite-infested pupae by worker bees, Varroa Sensitive Hygiene (VSH), leads to cannibalization of pupae with high DWV loads, thereby offering an alternative route for virus transmission. We used genetically tagged DWV to investigate virus transmission to and between worker bees following pupal cannibalisation under experimental conditions. We demonstrated that cannibalization of DWV-infected pupae resulted in high levels of this virus in worker bees and that the acquired virus was then transmitted between bees via trophallaxis, allowing circulation of Varroa-vectored DWV variants without the mites. Despite the known benefits of hygienic behaviour, it is possible that higher levels of VSH activity may result in increased transmission of DWV via cannibalism and trophallaxis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdcbd893e6c18b25d0e9c159ed399aa4Test
https://doi.org/10.1038/s41598-021-88649-yTest

7

A Vitellogenin Antibody in Honey Bees ( Apis mellifera ): Characterization and Application as Potential Biomarker for Insecticide Exposure

المؤلفون: Timm Hettich, Verena Christen, Karl Fent, Maren Vogel

المصدر: Environmental Toxicology and Chemistry. 38:1074-1083

مصطلحات موضوعية: 0301 basic medicine, Insecticides, endocrine system, animal structures, food.ingredient, Health, Toxicology and Mutagenesis, media_common.quotation_subject, Insect, 010501 environmental sciences, Guanidines, digestive system, 01 natural sciences, Antibodies, Neonicotinoids, Vitellogenins, 03 medical and health sciences, Vitellogenin, chemistry.chemical_compound, food, Hemolymph, Yolk, Animals, Environmental Chemistry, Amino Acid Sequence, 0105 earth and related environmental sciences, media_common, biology, fungi, Neonicotinoid, Clothianidin, Environmental Exposure, Bees, Pesticide, Worker bee, Thiazoles, 030104 developmental biology, chemistry, Biochemistry, biology.protein, Rabbits, Biomarkers

الوصف: The insect yolk precursor vitellogenin is a lipoglycoprotein synthesized and stored in the fat body and secreted into the hemolymph. In honey bees, vitellogenin displays crucial functions in hormone signaling, behavioral transition of nurse bees to foragers, stress resistance, and longevity in workers. Plant protection products such as neonicotinoids, pyrethroids, and organophosphates alter the transcriptional expression of vitellogenin. To assess plant protection product-induced alterations on the protein level, we developed a rabbit polyclonal vitellogenin antibody. After characterization, we assessed its specificity and vitellogenin levels in different tissues of worker bees. The vitellogenin antibody recognized full-length 180-kDa vitellogenin and the lighter fragment of 150 kDa in fat body, hemolymph, and brain. In hemolymph, a band of approximately 75 kDa was detected. Subsequent mass spectrometric analysis (liquid chromatography-mass spectrometry) confirmed the 180- and 150-kDa bands as vitellogenin. Subsequently, we evaluated vitellogenin expression in brain, fat body, and hemolymph on 24-h exposure of bees to 3 ng/bee to the neonicotinoid clothianidin. Full-length vitellogenin was upregulated 3-fold in the fat body, and the 150-kDa fragment was upregulated in the brain of exposed honey bees, whereas no alteration occurred in the hemolymph. Upregulation of the vitellogenin protein by the neonicotinoid clothianidin is in line with the previously shown induction of its transcript. We conclude that vitellogenin might serve as a potential biomarker for neonicotinoid and other pesticide exposure in bees. Environ Toxicol Chem 2019;00:1-10. © 2019 SETAC.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::530a9df870752240f2a2caae1ae4599eTest
https://doi.org/10.1002/etc.4383Test

8

Exposure to thiamethoxam during the larval phase affects synapsin levels in the brain of the honey bee

المؤلفون: Osmar Malaspina, Elaine C.M. Silva-Zacarin, Daiana Antonia Tavares, Roberta Cornélio Ferreira Nocelli, Thaisa Cristina Roat

المساهمون: Universidade Estadual Paulista (Unesp), Universidade Federal de São Carlos (UFSCar)

المصدر: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP

مصطلحات موضوعية: Insecticides, animal structures, Health, Toxicology and Mutagenesis, 0211 other engineering and technologies, Zoology, 02 engineering and technology, 010501 environmental sciences, Biology, 01 natural sciences, Neonicotinoids, chemistry.chemical_compound, Pesticide, mushroom body, antennal lobe, Animals, Nectar, 0105 earth and related environmental sciences, 021110 strategic, defence & security studies, Larva, fungi, Pupa, Public Health, Environmental and Occupational Health, Brain, food and beverages, General Medicine, Honey bee, Synapsin, Bees, Synapsins, Pollution, Honey bee development, Worker bee, chemistry, Bee pollen, Mushroom bodies, behavior and behavior mechanisms, Pollen, Apis mellifera, Thiamethoxam

الوصف: Made available in DSpace on 2019-10-06T16:06:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Thiamethoxam (TMX) is a neurotoxic insecticide widely used for insect pest control. TMX and other neonicotinoids are reported to be potential causes of honey bee decline. Due to its systematic action, TMX may be recovered in pollen, bee bread, nectar, and honey, which make bees likely to be exposed to contaminated diet. In this study, we used immunolabeling to demonstrate that sublethal concentrations of TMX decrease the protein levels of synapsin in the mushroom bodies (MBs) and the antennal lobes (ALs) of pupae and newly emerged worker bees that were exposed through the food to TMX during the larval phase. A decrease in the synapsin level was observed in the MBs of pupae previously exposed to 0.001 and 1.44 ng/µL and in newly emerged bees previously exposed to 1.44 ng/µL and no changes were observed in the optical lobes (OLs). In the ALs, the decrease was observed in pupae and newly emerged bees exposed to 1.44 ng/µL. Because the MBs and ALs are brain structures involved in stimuli reception, learning, and memory consolidation and because synapsin is important for the regulation of neurotransmitter release, we hypothesize that exposure to sublethal concentrations of TMX during the larval stage may cause neurophysiological disorders in honey bees. Universidade Estadual Paulista (UNESP) Centro de Estudos de Insetos Sociais (CEIS) Instituto de Biociências Campus Rio Claro Universidade Federal de São Carlos (UFSCar) Departamento de Biologia Laboratório de Ecotoxicologia e Biomarcadores em Animais (LEBA) Campus Sorocaba Universidade Federal de São Carlos (UFSCar) Departamento de Ciências da Natureza Matemática e Educação Centro de Ciências Agrárias Campus Araras Universidade Estadual Paulista (UNESP) Centro de Estudos de Insetos Sociais (CEIS) Instituto de Biociências Campus Rio Claro FAPESP: 2012/01498-0 FAPESP: 2012/13370-8 FAPESP: 2012/50197-2

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::304a99fb1ff3d73447ce33967dae91a8Test
https://doi.org/10.1016/j.ecoenv.2018.11.048Test

9

Sublethal Effects of the Insecticide Pyrifluquinazon on the European Honey Bee (Hymenoptera: Apidae)

المؤلفون: James M. Wilson, Thomas P. Kuhar, Troy D. Anderson

المصدر: Journal of Economic Entomology. 112:1050-1054

الوصف: Pyrifluquinazon (PQZ) is an Insecticide Resistance Action Committee (IRAC) Group 9 insecticide that has recently been registered for use in the United States for control of soft-bodied sucking insect pests. Although it has been classified as practically nontoxic to honey bees, Apis mellifera L. (Hymenoptera: Apidae), based on acute contact bioassays, additional information on sublethal effects of this insecticide on honey bees is lacking. Using a combination of laboratory assays with video movement tracking software and near-field evaluations of colonies foraging in a high-tunnel experiment, we determined that, when fed PQZ at a concentration of 84 mg active ingredient (ai)/liter (= ppm) in sugar water, a reduction in overall movement by the foraging worker bees was observed. However, when provided with honey reserves in the hive, honey bees rejected the PQZ-treated sugar water. These results indicate that, if ingested at levels of 84 mg ai/liter, PQZ could have a negative effect on honey bee behavior; however, honey bee workers appear to be able to detect the presence of PQZ in their food and reject it.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b70c5186cf11f4920cf9fc3ee172bed0Test
https://doi.org/10.1093/jee/toz014Test

10

The role of Vitellogenin in the transfer of immune elicitors from gut to hypopharyngeal glands in honey bees (Apis mellifera)

المؤلفون: Gro V. Amdam, Gyan Harwood, Dalial Freitak

المصدر: Journal of Insect Physiology. 112:90-100

الوصف: Female insects that survive a pathogen attack can produce more pathogen-resistant offspring in a process called trans-generational immune priming. In the honey bee (Apis mellifera), the egg-yolk precursor protein Vitellogenin transports fragments of pathogen cells into the egg, thereby setting the stage for a recruitment of immunological defenses prior to hatching. Honey bees live in complex societies where reproduction and communal tasks are divided between a queen and her sterile female workers. Worker bees metabolize Vitellogenin to synthesize royal jelly, a protein-rich glandular secretion fed to the queen and young larvae. We ask if workers can participate in trans-generational immune priming by transferring pathogen fragments to the queen or larvae via royal jelly. As a first step toward answering this question, we tested whether worker-ingested bacterial fragments can be transported to jelly-producing glands, and what role Vitellogenin plays in this transport. To do this, we fed fluorescently labelled Escherichia coli to workers with experimentally manipulated levels of Vitellogenin. We found that bacterial fragments were transported to the glands of control workers, while they were not detected at the glands of workers subjected to RNA interference-mediated Vitellogenin gene knockdown, suggesting that Vitellogenin plays a role in this transport. Our results provide initial evidence that trans-generational immune priming may operate at a colony-wide level in honey bees.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8f1d308e78c2751ce0e39ce5c085e22Test
https://doi.org/10.1016/j.jinsphys.2018.12.006Test

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