Effects of Storage and Passage of Bovine Luteal Endothelial Cells on Endothelin-1 and Prostaglandin F2.ALPHA. Production
| العنوان: | Effects of Storage and Passage of Bovine Luteal Endothelial Cells on Endothelin-1 and Prostaglandin F2.ALPHA. Production |
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| المؤلفون: | Seung-Hyung Lee, Tomas J. Acosta, Shin Yoshioka, Junichi Komiyama, Anna T. Grazul-Bilska, Dariusz J. Skarzynski, Kiyoshi Okuda |
| المصدر: | Journal of Reproduction and Development. 53:473-480 |
| بيانات النشر: | Japanese Society of Animal Reproduction, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | medicine.medical_specialty, Endothelium, Cell Culture Techniques, Prostaglandin, Luteal phase, Dinoprost, Andrology, chemistry.chemical_compound, Corpus Luteum, Internal medicine, Freezing, medicine, Animals, Bovine serum albumin, Cells, Cultured, Estrous cycle, Endothelin-1, biology, Tumor Necrosis Factor-alpha, fungi, Endothelial Cells, Endothelin 1, In vitro, medicine.anatomical_structure, Endocrinology, chemistry, biology.protein, Cattle, Female, Animal Science and Zoology, sense organs, Corpus luteum |
| الوصف: | To establish a storage system for isolated bovine luteal endothelial cells (LECs), we investigated the basal and tumor necrosis factor (TNF) alpha-stimulated production of endothelin-1 (ET-1) and prostaglandin (PG) F2alpha in unfrozen and frozen-thawed LECs until passage 10. LECs were obtained from developing corpora lutea (CL; days 5-7 of the estrous cycle) using enzymatic digestion and magnetic beads coated with lectin BS-1. The LECs were frozen at -80 C or further cultured and/or passaged until passage 10 in DMEM/Ham's F-12 supplemented with 10% calf serum. The hormonal productions of unfrozen and frozen/thawed LECs were compared through passages 2-10. When both the unfrozen and frozen/thawed cells reached confluence, the culture medium was replaced with fresh medium containing 0.1% bovine serum albumin (BSA), and the cells were incubated with TNFalpha (50 ng/ml) for 12 h. The basal productions of ET-1 and PGF2alpha by the unfrozen and frozen/thawed LECs were similar at passage 2. The basal production of PGF2alpha by LECs was not altered by passage and storage at -80 C, whereas the basal production of ET-1 decreased from passage 2 and 3 to passage 4 in the unfrozen LECs and from passage 2 to passage 3 in the frozen/thawed LECs. However, production of ET-1 by the unfrozen and frozen/thawed LECs was similar between passages 4-10 and passages 3-10, respectively. Exposure of LECs to TNFalpha increased (P0.05) ET-1 and PGF2alpha production by the unfrozen and frozen-thawed LECs in all passages examined. Thus, LECs obtained from developing CLs and stored until passage 10 can be used for study of the physiology of LECs in vitro. |
| تدمد: | 1348-4400 0916-8818 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::808100eecd6f86c407b9b50382c2850cTest https://doi.org/10.1262/jrd.18142Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....808100eecd6f86c407b9b50382c2850c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13484400 09168818 |
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