Consequences of a diet-induced obesity on the plasticity of the Pancreatic Intrinsic Nervous System and the nervous control of pancreatic endocrine secretion in young mice
| العنوان: | Consequences of a diet-induced obesity on the plasticity of the Pancreatic Intrinsic Nervous System and the nervous control of pancreatic endocrine secretion in young mice |
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| المؤلفون: | Blat, Sophie, Saade, Marie-Béatrice, MORIEZ, RAPHAEL, Aubert, Philippe, Neunlist, Michel |
| المساهمون: | ProdInra, Archive Ouverte, Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ToxAlim (ToxAlim), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, U913, Institut National de la Santé et de la Recherche Médicale (INSERM), Hotel Dieu, Université de Nantes (UN), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT) |
| المصدر: | Special Issue: Abstracts of the Joint International Neurogastroenterology and Motility Meeting. s2 (24), 2012; Joint International Neurogastroenterology and Motility Meeting, Bologne, ITA, 2012-09-06-2012-09-08, 131 Joint International Neurogastroenterology and Motility Meeting Joint International Neurogastroenterology and Motility Meeting, Sep 2012, Bologne, Italy. Wiley-Blackwell, Neurogastroenterology and Motility, 24 (s2), 2012, Neurogastroenterology and Motility Joint International Neurogastroenterology and Motility Meeting, Sep 2012, Bologne, Italy. Wiley-Blackwell, 24 (s2), 2012, Neurogastroenterology and Motility |
| بيانات النشر: | Wiley-Blackwell, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | [SDV] Life Sciences [q-bio], obésité, sécrétion, [SDV]Life Sciences [q-bio], animal modèle, pancréas, système nerveux, plasticité nerveuse |
| الوصف: | Session : Basic and translational session: Appetite regulation, satiety, obesity and nutritionSession : Basic and translational session: Appetite regulation, satiety, obesity and nutrition; Objective Pancreatic Intrinsic Nervous System (PINS) is involved in the control of pulsatility and amplitude of insulin secretion (IS) both altered in obese and type 2 diabetic patients. However, little is known about the role of the PINS during obesity and in the genesis of pancreatic endocrine dysfunctions. The aim of our study was to determine the impact of obesity upon the PINS and its functional consequences upon IS in young mice. Methods C57BL/6J mice aged 4 weeks received either a normal diet (ND; n=20) or a western (hyperlipidic hyperenergetic) diet (WD; n=20) for 12 weeks. 4 weeks old mice (n=16) were also used as initial controls (iC). After sacrifice, pancreases were removed and placed in short term organ culture for 1 hour. The impact of PINS upon endocrine function was studied by adding to the culture the nicotinic receptors agonist dymethylphenylPiperazinium (DMPP) in presence or absence of hexamethonium (nicotinic antagonist) or Nω-nitro-L-arginine methyl ester (L-NAME, inhibitor of NO synthase NOS) or sodium nitroprusside, (SNP, NO donor). Supernatant of culture was collected and frozen for further analysis of insulin by ELISA. Immunohistological analyses were performed to determine nerve fibers density and to phenotype neurons with choline acetyltransferase (Chat) and NOS antibodies. Results PINS stimulation by DMPP induced a time dependent increase in IS which was significantly larger in ND as compared to WD mice (P=0.01). Interestingly, the IS profile was identical in ND and iC mice (P=0.39). This secretion was fully blocked in presence of hexamethonium. Addition of L-Name significantly inhibited insulin release in ND mice (P |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::3c34ebc3df67477cd9278b9621b8a6baTest http://prodinra.inra.fr/record/192570Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.dedup.wf.001..3c34ebc3df67477cd9278b9621b8a6ba |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |