التفاصيل البيبلوغرافية
| العنوان: |
Detection of ruminant meat and bone meals in animal feed by real-time polymerase chain reaction: Result of an interlaboratory study |
| المؤلفون: |
Scott Reaney, Olivier Fumière, § Jonne Mensinga-Kruize, Christoph von Holst, Gilbert Berben, and Ana Boix, Gert van Duijn, Marta Prado |
| المساهمون: |
TNO Kwaliteit van Leven |
| المصدر: |
Journal of Agricultural and Food Chemistry, 18, 55, 7495-7501 |
| سنة النشر: |
2007 |
| مصطلحات موضوعية: |
animal food, mineral, Animal food, polymerase chain reaction, Mitochondrial/chromosomal DNA, animal, genetics, Bos, Food science, media_common, disease transmission, Minerals, Transmissible spongiform encephalopathy, Animal meals, article, methodology, Ruminants, Encephalopathy, Bovine Spongiform, bone meal, Feed, General Agricultural and Biological Sciences, Beef, biological product, Meat, Animal feed, Bovine spongiform encephalopathy, Food Contamination, Biology, MBM, Bone meal, Bovinae, medicine, media_common.cataloged_instance, Animals, Animalia, European union, bovine spongiform encephalopathy, Analytical research, Nutrition, Biological Products, business.industry, General Chemistry, DNA, Biological product, medicine.disease, Animal Feed, Biotechnology, cattle, Bovidae, business, bovids, Food contaminant, Real-time PCR |
| الوصف: |
The commercialization of animal feeds infected by prions proved to be the main cause of transmission of bovine spongiform encephalopathy (BSE). Therefore, feed bans were enforced, initially for ruminant feeds, and later for all feeds for farmed animals. The development and validation of analytical methods for the species-specific detection of animal proteins in animal feed has been indicated in the TSE (Transmissible Spongiform Encephalopathies) Roadmap (European Commission. The TSE (Transmissible Spongiform Encephalopathy) roadmap. URL: http://europa.eu.int/comm/food/food/biosafety/bse/roadmap_en.pdfTest, 2005) as the main condition for lifting the extended feed ban. Methods based on polymerase chain reaction (PCR) seem to be a promising solution for this aim. The main objective of this study was to determine the applicability of four different real-time PCR methods, developed by three National expert laboratories from the European Union (EU), for the detection and identification of cattle or ruminant species in typical compound feeds, fortified with meat and bone meals (MBM) from different animal species at different concentration levels. The MBM samples utilized in this study have been treated using the sterilization condition mandatory within the European Union (steam pressure sterilization at 133°C, 3 bar, and 20 min), which is an additional challenge to the PCR methods evaluated in this study. The results indicate that the three labs applying their PCR methods were able to detect 0.1% of cattle MBM, either alone or in mixtures with different materials such as fishmeal, which demonstrates the improvement made by this technique, especially when compared with results from former interlaboratory studies. © 2007 American Chemical Society. |
| اللغة: |
English |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9170c83b2f8f8ea9793676fbc6d07bbTest
http://resolver.tudelft.nl/uuid:ec598db0-6948-4303-a2dc-80db28798e54Test |
| حقوق: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....a9170c83b2f8f8ea9793676fbc6d07bb |
| قاعدة البيانات: |
OpenAIRE |
