المؤلفون: Eirin, Maria Emilia, Carignano, Hugo Adrian, Shimizu, Ernesto, Pando, Maria de los Angeles, Zumarraga, Martin Jose, Magnano, Gabriel, Macías, Analía, Garbaccio, Sergio Gabriel, Huertas, Pablo Sebastian, Morsella, Claudia Graciela, Ferrara Muñiz, Ximena, Cataldi, Angel Adrian, Paolicchi, Fernando, Poli, Mario Andres

المصدر: Research in Veterinary Science 130 : 118-125 (June 2020)

مصطلحات موضوعية: Ganado Bovino, Enfermedades de los Animales, Polimorfismo, Tuberculosis, Mycobacterium bovis, Cattle, Animal Diseases, Polymorphism, Mycobacterium bovis Infections, Antigens, Infeccion por Mycobacterium bovis, Antígenos, Tuberculosis Bovina, Bovine Leucocyte Antigen

الوصف: Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and disseminated worldwide. In Argentina, the highest prevalence occurs in dairy areas. BoLA DRB3.2 is related to the adaptive immunity in mycobacterial infections. Genetic polymorphisms of this marker have been associated with resistance or susceptibility to bovine diseases. We evaluated the association between BoLA DRB3.2 polymorphisms and bTB pathology scores in dairy and beef cattle breeds of Argentina. Most bovines exhibited visible lesions compatible with tuberculosis and, furthermore, 150 (85.7%) were also positive by bacteriology. A pathology index showed a variable degree of disease, from 3 to 76 (median pathology score = 9 (IQR: 7–15)). Thirty-five BoLA DRB3.2 alleles were identified with an associated frequency from 16% to 0.3%, distributed 73% (n = 128) in heterozygosis and 27% (n = 47) in homozygosis, with 12 BoLA DRB3.2 alleles (*0101, *1101, *1501, *0201, *2707 *1001, *1002, *1201, *14011, *0501 *0902 and *0701) representing the 74.7% of the population variability. A functional analysis grouped them in 4 out of 5 clusters (A-D), suggesting a functional overlapping. Among the 90 identified genotypes, *1101/*1101, *1101/*1501 and *0101/*0101 were the most frequent (10%, 8.9% and 8.9%, respectively). No association was detected between the pathology scores and a specific DRB3.2 allele (p > .05). Animals infected with M. bovis spoligotype SB0153 showed a significantly higher pathology score than those affected by the spoligotype SB0145 (p = .018). Furthermore, the Aberdeen Angus breed exhibited highest pathological scores (p < .0001), which were associated with disseminated lesion, thus suggesting that the host component could be important to the disease progression. ; Instituto de Biotecnología ; Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Carignano, Hugo Adrian. Instituto Nacional de ...

وصف الملف: application/pdf

العلاقة: http://hdl.handle.net/20.500.12123/7706Test; https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624Test; https://doi.org/10.1016/j.rvsc.2020.03.001Test

الإتاحة: https://doi.org/20.500.12123/7706Test
https://doi.org/10.1016/j.rvsc.2020.03.001Test
https://hdl.handle.net/20.500.12123/7706Test
https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624Test

المؤلفون: Eirin, Maria Emilia, Macías, Analía, Magnano, Gabriel, Morsella, Claudia Graciela, Mendez, Laura Luján, Blanco, Federico Carlos, Bianco, María Veronica, Severina, Walter, Alito, Alicia Elsa, Pando, Maria de los Angeles, Singh, Mahavir G., Spallek, Ralph, Paolicchi, Fernando, Bigi, Fabiana, Cataldi, Angel Adrian

المصدر: Tuberculosis 95 (6) : 795-801 (December 2015)

مصطلحات موضوعية: Enfermedades de los Animales, Ganado Bovino, Tuberculosis, Infeccion por Mycobacterium bovis, Mycobacterium Bovis, Antígenos, Interferonas, Diagnóstico, Animal Diseases, Cattle, Mycobacterium bovis Infections, Antigens, Interferons, Diagnosis

الوصف: Bovine tuberculosis (bTB) is a common zoonotic disease, caused by Mycobacterium bovis (M. bovis), responsible for significant economic losses worldwide. Its diagnosis is based on the detection of cell mediated immunity under the exposure to protein purified derivative tuberculin (PPD), a complex and poorly characterized reagent. The cross-reactivity to non-tuberculous mycobacterium species (false-positive results) has been crucial to develop a more proper antigen. In the present study, we selected six M. bovis Open Reading Frames (Mb1992, Mb2031c, Mb2319, Mb2843c, Mb2845c and Mb3212c) by in-silico analysis and evaluated them in experimental and natural infection; none of these antigens had been previously assessed as diagnostic antigens for bTB. The reactivity performance was tested in animals with both positive and negative Tuberculin Skin Test (TST) results as well as in cattle infected with Mycobacterium avium subesp. paratuberculosis (MAP). The six recombinant antigens individually induced an IFN-γ response, with overall responder frequency ranging from 18.3 to 31%. Mb2845c was the most valuable antigen with the potential to discriminate TST-positive cattle from either TST-negative or MAP infected animals. Mb2845c showed similar performance to that observed with ESAT-6 and PPD-B among TST and MTC specific-PCR positive animals, although this result needs to be proven in further studies with a higher sample size. Our data confirm the feacibility to implement bioinformatic screening tools and suggest Mb2845c as a potential diagnostic antigen to be tested in protein cocktails to evaluate their contribution to bTB diagnosis. ; Instituto de Biotecnología ; Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina ; Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina ; Fil: Morsella, Claudia ...

وصف الملف: application/pdf

العلاقة: https://www.sciencedirect.com/science/article/pii/S1472979215207688Test; http://hdl.handle.net/20.500.12123/4585Test; https://doi.org/10.1016/j.tube.2015.07.009Test

الإتاحة: https://doi.org/20.500.12123/4585Test
https://doi.org/10.1016/j.tube.2015.07.009Test
https://hdl.handle.net/20.500.12123/4585Test
https://www.sciencedirect.com/science/article/pii/S1472979215207688Test