مؤتمر
Simultaneous detection and quantification of angiotensin I, II, 1-7 and 1-9 by LC-MS/MS in human plasma
| العنوان: | Simultaneous detection and quantification of angiotensin I, II, 1-7 and 1-9 by LC-MS/MS in human plasma |
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| المؤلفون: | Huyghebaert, Loreen, Demeuse, Justine, Grifnee, Elodie, Massonnet, Philippe, Farre Segura, Jordi, Peeters, Stéphanie, Le Goff, Caroline, Cavalier, Etienne |
| المصدر: | International Mass Spectrometry Conference (IMSC) 2022, Maastricht, Netherlands [NL], 29/08/22 - 02/09/22 |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | angiotensin, LC-MS/MS, Human health sciences, Laboratory medicine & medical technology, Sciences de la santé humaine, Médecine de laboratoire & technologie médicale |
| الوصف: | Recent studies showed that angiotensin-converting 2 (ACE2) is used by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a cellular entry receptor. SARS-CoV-2 causes down regulation of ACE2 leading to renin-angiotensin-aldosterone system (RAAS) major imbalance. This is an essential element of unfavourable evolution in patients with COVID-19. With lower level of ACE2, cleavage of And I and And II is decrease and therefore, And 1-7 and And 1-9 levels are decreased. The development of a quantitative method for these angiotensins is particularly interesting in the context of the prognosis/follow-up of patients with COVID-19. Based on this, this work aims at assessing angiotensins profile variations occurring over time with patients COVID-19 + using a LC-MS/MS method. In this project, plasma samples are prepared with an microelution MAX plate before injection on a Vexera X2 UPLC (Shimadzu Corporation, Kyoto, Japan) coupled to a QT5500 mass spectrometer (Scion, CA, USA) fitted with an IonDriveTMTurbo V ion source and using electro spray ionisation in positive mode. The mobile phase are composed of water (+0,4% formic acid) and of acetonitrile (+0,4% formic acid). |
| نوع الوثيقة: | conference poster not in proceedings http://purl.org/coar/resource_type/c_18coTest conferencePoster peer reviewed |
| اللغة: | English |
| الوصول الحر: | https://orbi.uliege.be/handle/2268/295374Test |
| حقوق: | open access http://purl.org/coar/access_right/c_abf2Test info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsorb.295374 |
| قاعدة البيانات: | ORBi |
| الوصف غير متاح. |