Serum from Jiao-Tai-Wan treated rats increases glucose consumption by 3T3-L1 adipocytes through AMPK pathway signaling
| العنوان: | Serum from Jiao-Tai-Wan treated rats increases glucose consumption by 3T3-L1 adipocytes through AMPK pathway signaling |
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| المؤلفون: | Xiong Lu, Yin-Qiang Sun, Xiao-Yu Zhong, Hui-Jiao Li, Na Hu, Lin Yuan, Min Lin, Peng Tang, Min Lu |
| المصدر: | Bioscience Reports |
| بيانات النشر: | Portland Press Ltd., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, AMPK, Male, Serum, Glucose uptake, Type 2 diabetes, AMP-Activated Protein Kinases, Biochemistry, Mice, 0302 clinical medicine, Adipocytes, Research Articles, Glucose Transporter Type 4, biology, Chemistry, 3T3 Cells, 030220 oncology & carcinogenesis, medicine.drug, Research Article, medicine.medical_specialty, Biophysics, Cell Line, 03 medical and health sciences, Insulin resistance, Internal medicine, medicine, Animals, Hypoglycemic Agents, Molecular Biology, Dexamethasone, Jiao-Tai-Wan Decoction, Glucose transporter, 3T3-L1, Cell Biology, medicine.disease, Rats, Disease Models, Animal, 030104 developmental biology, Endocrinology, Glucose, Diabetes Mellitus, Type 2, Hyperglycemia, biology.protein, GLUT4, Drugs, Chinese Herbal |
| الوصف: | Type 2 diabetes (T2DM) is characterized by hyperglycemia resulting from insulin resistance. Jiao-Tai-Wan (JTW), a traditional Chinese medicine consisting of a 10:1 formulation of Rhizoma Coptidis (RC) and Cortex Cinnamomi (cinnamon) was shown to have hypoglycemic efficacy in a type 2 diabetic mouse model. Here we investigated whether glucose consumption by insulin-resistant adipocytes could be modulated by serum from JTW-treated rats, and if so, through what mechanism. JTW-medicated serum was prepared from rats following oral administration of JTW decoction twice a day for 4 days. Fully differentiated 3T3-L1 adipocytes – rendered insulin resistance by dexamethasone treatment – were cultured in medium containing JTW-medicated rat serum. JTW-medicated serum treatment increased glucose uptake, up-regulated levels of phosphorylated adenosine 5′-monophoshate-activated protein kinase (p-AMPK), and stimulated expression and translocation of glucose transporter 4 (GLUT4). JTW-medicated serum induced significantly greater up-regulation of p-AMPK and GLUT4 than either RC or cinnamon-medicated serum. JTW-medicated serum induced effects on 3T3-L1 adipocytes could be partially inhibited by treatment with the AMPK inhibitor compound C. In conclusion, JTW-medicated serum increased glucose consumption by IR adipocytes partially through the activation of the AMPK pathway, and JTW was more effective on glucose consumption than either RC or cinnamon alone. |
| اللغة: | English |
| تدمد: | 1573-4935 0144-8463 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96dec518301c022439d0873d1c1a0911Test http://europepmc.org/articles/PMC6449522Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....96dec518301c022439d0873d1c1a0911 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15734935 01448463 |
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