The influence of sodium ion binding on factor IXa activity
| العنوان: | The influence of sodium ion binding on factor IXa activity |
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| المؤلفون: | Kota N. Gopalakrishna, Alireza R. Rezaie |
| المصدر: | Thrombosis and Haemostasis. 95:936-941 |
| بيانات النشر: | Georg Thieme Verlag KG, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Antithrombins, Factor IXa, chemistry.chemical_compound, Thrombin, Allosteric Regulation, Prothrombinase, medicine, Humans, Binding site, Factor VIIIa, Binding Sites, Factor X, Sodium, Antithrombin, Biological activity, Hematology, Blood Coagulation Factors, Neoplasm Proteins, Cysteine Endopeptidases, Kinetics, Chromogenic Compounds, Biochemistry, chemistry, Calcium, Sodium ion binding, Oligopeptides, Protein Binding, medicine.drug |
| الوصف: | SummarySodium plays an important role in modulating both the amidolytic and proteolytic activities of thrombin. By contrast, while the optimal amidolytic activity of factor Xa requires Na+, the proteolytic activity of factor Xa in the prothrombinase complex is minimally affected by the monovalent cation. In this study, we analyzed the effect of Na+ on the amidolytic and proteolytic activity of factor IXa in the absence and presence of factor VIIIa. Factor IXa exhibited normal activity towards a fIXa-specific chromogenic substrate and antithrombin in the presence of physiological concentrations of Ca2+ with no obvious requirement for Na+ in either reaction. Further studies revealed that factor IXa binds to its cofactor factor VIIIa with a normal affinity in the absence of Na+ and that the catalytic function in the intrinsic Xase complex is also independent of Na+ in the presence of physiological concentrations of Ca2+.These results suggest that unlike the important role that Na+ plays in modulating the macromolecular substrate specificity of thrombin, the monovalent cation is not required for the physiological function of factor IXa in the intrinsic Xase complex. |
| تدمد: | 2567-689X 0340-6245 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c1c2771bcf862df3c01abd66c6b101cTest https://doi.org/10.1160/th06-03-0156Test |
| رقم الانضمام: | edsair.doi.dedup.....8c1c2771bcf862df3c01abd66c6b101c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2567689X 03406245 |
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