التفاصيل البيبلوغرافية
| العنوان: |
Carrier-bound nonallergenic Der p 2 peptides induce Ig G antibodies blocking allergen-induced basophil activation in allergic patients. |
| المؤلفون: |
Chen, K.-W., Focke-Tejkl, M., Blatt, K., Kneidinger, M., Gieras, A., Dall'Antonia, F., Faé, I., Fischer, G., Keller, W., Valent, P., Valenta, R., Vrtala, S. |
| المصدر: |
Allergy; May2012, Vol. 67 Issue 5, p609-621, 11p |
| مصطلحات موضوعية: |
ALLERGIES, IMMUNOGLOBULINS, CELLULAR immunity, HOUSE dust mites, T cells, IMMUNOLOGIC diseases |
| مستخلص: |
Background More than 90% of house dust mite-allergic patients are sensitized to the major Dermatophagoides pteronyssinus allergen, Der p 2. The aim of this study was to develop and characterize an allergy vaccine based on carrier-bound Der p 2 peptides, which should allow reducing Ig E- and T-cell-mediated side-effects during specific immunotherapy ( SIT). Methods Five Der p 2 peptides ( P1- P5) were synthesized and analyzed regarding Ig E reactivity and allergenic activity. Lymphoproliferative and cytokine responses induced with Der p 2 and Der p 2 peptides were determined in peripheral blood mononuclear cells from mite-allergic patients. Der p 2-specific Ig G antibodies induced with carrier-bound Der p 2 peptides in mice and rabbits were tested for their capacity to inhibit Ig E binding and basophil activation in allergic patients. Results Of five overlapping peptides ( P1- P5) covering the Der p 2 sequence, two peptides ( P2 and P4) were identified, which showed no relevant Ig E reactivity, allergenic activity, and induced lower Der p 2-specific T-cell activation than Der p 2. However, when coupled to a carrier, P2 and P4 induced Der p 2-specific Ig G antibodies in animals, which inhibited allergic patients' Ig E binding to the allergen and allergen-induced basophil activation similar as antibodies induced with Der p 2. Conclusions Carrier-bound Der p 2 peptides should allow avoiding Ig E-mediated side-effects, and because of their low potential to activate allergen-specific T cells, they may reduce late-phase side-effects during SIT. Further, these peptides may be also useful for prophylactic vaccination. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
