التفاصيل البيبلوغرافية
| العنوان: |
Effect of omega-3 fatty acid supplementation on resolvin (RvE1)-mediated suppression of inflammation in a mouse model of asthma. |
| المؤلفون: |
Siddiquee, Armaan, Patel, Mehaben, Rajalingam, Sahith, Narke, Deven, Kurade, Mangesh, Ponnoth, Dovenia S. |
| المصدر: |
Immunopharmacology & Immunotoxicology; Apr2019, Vol. 41 Issue 2, p250-257, 8p |
| مصطلحات موضوعية: |
OMEGA-3 fatty acids, LIPOXINS, CELL analysis, FATTY acids, FISH oils, ASTHMA, INFLAMMATION |
| مستخلص: |
Objective: ResolvinE1 (RvE1), an endogenous lipid mediator derived from omega 3 fatty acids contributes to resolution of allergic inflammatory responses. We investigated effects of RvE1 (R) and omega 3 fatty acids (O) on airway reactivity and inflammation using allergic mice. Methods: Mice were divided into control (nonasthmatic; CON) and allergen sensitized-challenged (asthmatic; SEN) groups, and were sensitized i.p. on days 1, 6 with 0.2 μg ovalbumin (OVA) followed by 5% OVA aerosol challenges on days 11–13. RvE1 was administered i.p. postallergen challenge, while omega 3 fatty acids (fish oil) were administered via oral gavage once daily (days 1–13). Whole body plethysmography and bronchoalveolar lavage (BAL) studies were performed on day 14. Results: RvE1 attenuated airway responsiveness to methacholine (48 mg/ml) in treated asthmatic mice vs. nontreated (150 ± 27.88% in SEN vs. 54 ± 7.52% in SEN + R, p <.05). No difference was observed with omega-3 supplementation (115 ± 19.28% in SEN + O) or treatment with both RvE1 and omega 3 fatty acids (39 ± 12.37% in SEN + R + O vs. 54 ± 7.52% in SEN + R). Differential BAL cell analysis showed that RvE1 decreased eosinophils and neutrophils in SEN mice (p <.005) while no difference was observed with omega-3 fatty acids. SEN + R + O group had similar results as RvE1 treated mice, suggesting that only RvE1 attenuated inflammation. Conclusions: RvE1 attenuated airway responsiveness and inflammation in asthmatic mice. Omega-3 fatty acids, although a precursor for RvE1 formation, had no additive effects on RvE1 decreases in airway inflammation and airway reactivity. Our data suggests that omega-3 supplementation has little effect on airway inflammation and reactivity in our model of asthma. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
