التفاصيل البيبلوغرافية
| العنوان: |
Harmine promotes periodontal ligament cell‐induced tissue regeneration. |
| المؤلفون: |
Lim, H‐C1,2, Cha, B‐Y3, Song, S. U.4, Yun, J‐H5,6 grayheron@hanmail.net |
| المصدر: |
Oral Diseases. Apr2018, Vol. 24 Issue 3, p456-464. 9p. 2 Diagrams, 1 Chart, 4 Graphs. |
| مصطلحات موضوعية: |
*CELL proliferation, *ALKALOIDS, *ANIMAL experimentation, *BONE growth, *COLLAGEN, *ECTOPIC pregnancy, *GENE expression, *IMMUNOASSAY, *MICE, *PERIODONTAL ligament, *POLYMERASE chain reaction, *REGENERATION (Biology), *STAINS & staining (Microscopy), *WESTERN immunoblotting, *FETAL development, *ECTOPIC tissue |
| مستخلص: |
Abstract: Objective: to investigate whether harmine has a promotive effect on human periodontal ligament cells (hPDLCs)‐induced tissue regeneration. Materials and Methods: Various concentrations of harmine on hPDLCs proliferation were tested. Osteogenic and cementogenic characteristics were examined in hPDLC/rhBMP‐2 and hPDLC/harmine by alizarin red S staining, real‐time PCR, and Western blotting assay. The activity of harmine was investigated in an ectopic transplantation nude mouse model. Results: We determined that 10 μM of harmine was the threshold concentration. hPDLC/harmine showed similar mineralized nodule formation in alizarin S staining compared to hPDLC/rhBMP‐2. In real‐time PCR, the highest gene expression level was observed for Runx2 in hPDLC/harmine at all time points. The level of CEMP‐1 in hPDLC/harmine was higher at 7 days than hPDLCs alone. Thicker band of Runx2 in hPDLC/harmine was observed than in hPDLC/rhBMP‐2 at 7 days by Western blotting. The band for CEMP‐1 in hPDLC/harmine was thicker than hPDLCs alone at both 7 and 14 days. In ectopic transplantation, hPDLCs with harmine showed a comparable amount of mineralized tissue formation compared to rhBMP‐2. hPDLCs with harmine or rhBMP‐2 formed both bone and cementum‐like tissue with Sharpey's fiber‐like collagen insertion. Conclusion: Harmine can be a potential candidate for promoting hPDLCs‐induced tissue regeneration. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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