Immunoassays for the quantification of ALK and phosphorylated ALK support the evaluation of on‐target ALK inhibitors in neuroblastoma
| العنوان: | Immunoassays for the quantification of ALK and phosphorylated ALK support the evaluation of on‐target ALK inhibitors in neuroblastoma |
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| المؤلفون: | Tucker, Elizabeth R., Tall, Jennifer R., Danielson, Laura S., Gowan, Sharon, Jamin, Yann, Robinson, Simon P., Banerji, Udai, Chesler, Louis |
| المصدر: | Molecular Oncology |
| بيانات النشر: | John Wiley and Sons Inc., 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Immunoassay, Pyridines, Receptor Protein-Tyrosine Kinases, Neoplasm Proteins, Neuroblastoma, Pyrimidines, ALK, Crizotinib, hemic and lymphatic diseases, Humans, Pyrazoles, Anaplastic Lymphoma Kinase, Sulfones, Drug Screening Assays, Antitumor, Phosphorylation, Research Articles, Research Article, HeLa Cells |
| الوصف: | Targeted inhibition of anaplastic lymphoma kinase (ALK) is a successful approach for the treatment of many ALK-aberrant malignancies; however, the presence of resistant mutations necessitates both the development of more potent compounds and pharmacodynamic methods with which to determine their efficacy. We describe immunoassays designed to quantitate phosphorylation of ALK, and their use in preclinical models of neuroblastoma, a pediatric malignancy in which gain-of-function ALK mutations predict a poor overall outcome to conventional treatment. Validation of the immunoassays is presented using a panel of neuroblastoma cell lines and evidence of on-target ALK inhibition provided by treatment of a genetically engineered murine model of neuroblastoma with two clinical ALK inhibitors, crizotinib and ceritinib, highlighting the superior efficacy of ceritinib. |
| اللغة: | English |
| تدمد: | 1878-0261 1574-7891 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid________::4e3f15437383c1ee744ad0b5ef20cabeTest http://europepmc.org/articles/PMC5537911Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid..........4e3f15437383c1ee744ad0b5ef20cabe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18780261 15747891 |
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