المؤلفون: Elena Aloisio, Giulia Colombo, Alberto Dolci, Mauro Panteghini, Claudia Arrigo

المصدر: Clinica Chimica Acta; International Journal of Clinical Chemistry

مصطلحات موضوعية: Clinical Biochemistry, AST, aspartate aminotransferase, Liver injury, Systemic inflammation, Biochemistry, Gastroenterology, law.invention, chemistry.chemical_compound, fluids and secretions, law, polycyclic compounds, Medicine, IL-6, interleukin-6, LDH, lactate dehydrogenase, biology, Liver Diseases, virus diseases, Alanine Transaminase, R2, coefficient of determination, General Medicine, ICU, intensive care unit, Intensive care unit, P-5′-P, pyridoxal-5-phosphate, CRP, C-reactive protein, Alkaline phosphatase, medicine.symptom, medicine.medical_specialty, Aspartate aminotransferase, digestive system, Article, SARS-CoV-2, severe acute respiratory syndrome coronavirus 2 COVID-19. 2019 coronavirus disease, ALT, alanine aminotransferase, Lactate dehydrogenase, Internal medicine, Humans, Clinical significance, IFCC, International Federation of Clinical Chemistry and Laboratory Medicine, Aspartate Aminotransferases, IQR, interquartile range, ALP, alkaline phosphatase, SARS-CoV-2, business.industry, Biochemistry (medical), URL, upper reference limit, COVID-19, medicine.disease, digestive system diseases, OR, odds ratio, CI, confidence interval, HI, hemolysis index, GGT, γ-glutamyltransferase, R, correlation coefficient, Alanine transaminase, chemistry, biology.protein, Creatine kinase, business, CK, creatine kinase

الوصف: Background Aspartate aminotransferase (AST) is often increased in COVID-19 and, in some studies, AST abnormalities were associated with mortality risk. Methods 2054 hospitalized COVID-19 patients were studied. To identify sources of AST release, correlations between AST peak values and other biomarkers of tissue damage, i.e., alanine aminotransferase (ALT) for hepatocellular damage, creatine kinase (CK) for muscle damage, lactate dehydrogenase for multiorgan involvement, alkaline phosphatase and γ-glutamyltransferase for cholestatic injury, and C-reactive protein (CRP) for systemic inflammation, were performed and coefficients of determination estimated. The role of AST to predict death and intensive care unit admission during hospitalization was also evaluated. All measurements were performed using standardized assays. Results AST was increased in 69% of patients. Increases could be fully explained by summing the effects of hepatocellular injury [AST dependence from ALT, 66.8% [95% confidence interval (CI): 64.5–69.1)] and muscle damage [AST dependence from CK, 42.6% (CI: 39.3–45.8)]. We were unable to demonstrate an independent association of AST increases with worse outcomes. Conclusion The mechanisms for abnormal AST in COVID-19 are likely multifactorial and a status related to tissue suffering could play a significant role. The clinical significance of AST elevations remains unclear.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3d1d325846e5aacf52eb8f0ef551a94Test
https://doi.org/10.1016/j.cca.2021.08.012Test

المؤلفون: Missinhoun Dagoudo, Ezra Tumukunde Mutebi, Jun Qiang, Yifan Tao, Haojun Zhu, Tlou Kevin Ngoepe, Pao Xu

مصطلحات موضوعية: Metabolic Theory of Ecology and Climate Change Impacts, Ecology, FOS Biological sciences, Environmental Science, Physical Sciences, Immunological Responses in Aquatic Organisms, Immunology, FOS Clinical medicine, Immunology and Microbiology, Life Sciences, Metabolism and Nutrition in Aquaculture Feeds, Aquatic Science, Agricultural and Biological Sciences, Fish Immunology, Temperature, Metabolic Rate, Stress Response, Catfish, Malondialdehyde, Internal medicine, Endocrinology, Superoxide dismutase, Glycogen, Immune system, Biology, Lactate dehydrogenase, Alanine transaminase, Oxidative stress, Biochemistry, Medicine

الوصف: This study investigated the effect of heat stress on the physiological parameters, oxidation resistance ability and immune responses in juvenile hybrid yellow catfish. Heat stress group exposed to 35 °C and control to 28 °C. Blood and liver were sampled at different hours' post-exposure. Results showed that red blood cell (RBC), white blood cell (WBC) counts, Hemoglobin (HGB) levels and hematocrit (HCT) values increased significantly (P < 0.05) post-exposure to heat stress. This indicates the increase of cell metabolism. Serum alanine aminotransferase (ALT) and aspartate transaminase (AST) activities, total cholesterol (TC), total protein (TP), triglyceride (TG) and glucose increased significantly (P < 0.05) indicating the need to cope with stress and cell damage. Liver TC, TG, COR hormone, C3 complement increased significantly from 24 to 96 h. Heat stress mostly affects the hepatic antioxidant and immune resistance functions, resulting in increments of cortisol levels, lysozyme, superoxide dismutase ... : بحثت هذه الدراسة في تأثير الإجهاد الحراري على المعلمات الفسيولوجية والقدرة على مقاومة الأكسدة والاستجابات المناعية في سمك السلور الأصفر الهجين اليافع. مجموعة الإجهاد الحراري المعرضة إلى 35 درجة مئوية والتحكم إلى 28 درجة مئوية. تم أخذ عينات من الدم والكبد في ساعات مختلفة بعد التعرض. أظهرت النتائج أن عدد خلايا الدم الحمراء وكريات الدم البيضاء ومستويات الهيموغلوبين وقيم الهيماتوكريت زادت بشكل كبير (P < 0.05) بعد التعرض للإجهاد الحراري. وهذا يشير إلى زيادة التمثيل الغذائي للخلايا. زادت أنشطة ناقلة أمين ألانين المصل (ALT) وناقلة أمين الأسبارتات (AST)، والكوليسترول الكلي (TC)، والبروتين الكلي (TP)، والدهون الثلاثية (TG) والجلوكوز بشكل كبير (P < 0.05) مما يشير إلى الحاجة إلى التعامل مع الإجهاد وتلف الخلايا. الكبد تك، تغ، كور هرمون، C3 مكمل زيادة كبيرة من 24 إلى 96 ساعة. يؤثر الإجهاد الحراري في الغالب على وظائف مضادات الأكسدة الكبدية والمقاومة المناعية، مما يؤدي إلى زيادات في مستويات الكورتيزول والليزوزيم وفوق أكسيد ديسموتاز (SOD) وأنشطة إنزيم الكاتالاز (CAT). تشير زيادة مالونديالديهيد (MDA)، الفوسفاتيز القلوي ...

العلاقة: https://dx.doi.org/10.60692/67gk4-cqm17Test

الإتاحة: https://doi.org/10.60692/pesam-c6g5410.60692/67gk4-cqm17Test

المؤلفون: Oluwaferanmi Adefolaju, Julius Arannilewa, Ayokanmi Olowo

مصطلحات موضوعية: Diabetes, Oxidative Stress, and Antioxidants, Endocrinology, Diabetes and Metabolism, Medicine, Health Sciences, Impact of Fructose on Metabolic Health, Epidemiology and Management of NAFLD, Epidemiology, Diabetes, Alkaline phosphatase, Aspartate transaminase, Small intestine, Alanine transaminase, Alloxan, Lactate dehydrogenase, Traditional medicine, Oral administration, Chemistry, Pharmacology, Biochemistry, Enzyme, Biology, Diabetes mellitus, Internal medicine, Endocrinology

الوصف: The stem barks of Securidaca longepedunculata are used traditionally across Africa for the treatment of diabetes, cancer, metabolic diseases and asthma. This study was aimed at investigating the potential enzyme activities of the S. longepedunculata on the small intestine. Aqueous extracts of S. longepedunculata were tested in vivo on animal models: A total of 12 Wister rats were assigned into four (I–IV) groups of three animals each. Group I served as the control and was administered 0.5mL of distilled water. Groups II–IV were given 0.5, 1 and 2mg/kg body weight of S. longepedunculata stem bark extracts. The activities of the following enzymes, Alanine transaminase (ALT), Lactate dehydrogenase (LDH), Alkaline phosphatase (ALP), Aspartate transaminase (AST), were assayed in the small intestine. The result revealed a significant reduction in ALP and LDH. This gives an indication that the administration of aqueous extract of securidaca longepedunculata can elicit detrimental effect in the small intestine of ... : تُستخدم لحاء الساق من Securidaca longepedunculata تقليديًا في جميع أنحاء أفريقيا لعلاج مرض السكري والسرطان والأمراض الأيضية والربو. هدفت هذه الدراسة إلى التحقيق في أنشطة الإنزيم المحتملة لـ S. longepedunculata على الأمعاء الدقيقة. تم اختبار المستخلصات المائية من S. longepedunculata في الجسم الحي على النماذج الحيوانية: تم تخصيص ما مجموعه 12 فأرًا ويسترًا في أربع مجموعات (من الأول إلى الرابع) من ثلاثة حيوانات لكل منها. عملت المجموعة الأولى كمجموعة تحكم وتم إعطاؤها 0.5 مل من الماء المقطر. أعطيت المجموعات من الثاني إلى الرابع 0.5 و 1 و 2 ملغم/كغم من وزن الجسم من مستخلصات لحاء ساق S. longepedunculata. تم اختبار أنشطة الإنزيمات التالية، ناقلة أمين الألانين (ALT)، نازعة هيدروجين اللاكتات (LDH)، الفوسفاتيز القلوي (ALP)، ناقلة أمين الأسبارتات (AST)، في الأمعاء الدقيقة. كشفت النتيجة عن انخفاض كبير في ALP و LDH. وهذا يعطي مؤشرا على أن إعطاء مستخلص مائي من سيكيوريداكا لونجيبدونكولاتا يمكن أن يثير تأثيرا ضارا في الأمعاء الدقيقة للفئران المهق. كما تظهر النتيجة التي تم الحصول عليها في التحليل النوعي وجود مواد كيميائية ...

العلاقة: https://dx.doi.org/10.60692/0kzc6-qm916Test

الإتاحة: https://doi.org/10.60692/kj8tn-2t32910.60692/0kzc6-qm916Test

المؤلفون: MA Yuan-yuan, YU Meng, Li Xiao-fang, XiE Zhi-ying, Li Xue-ying, ZHANG Zhe, YUAN Yun, WANG Zhao-xia

المصدر: Chinese Journal of Contemporary Neurology & Neurosurgery; May2019, Vol. 19 Issue 5, p329-335, 7p

مصطلحات موضوعية: ASPARTATE aminotransferase, BIOMARKERS, CHI-squared test, CONFIDENCE intervals, CREATINE kinase, DIFFERENTIAL diagnosis, ENZYMES, VIRAL hepatitis, LACTATE dehydrogenase, MUSCLE diseases, MUSCULAR dystrophy, SERUM, STATISTICS, DATA analysis, ALANINE aminotransferase, RECEIVER operating characteristic curves, SKELETAL muscle

مستخلص: Objective To explore the expression differences of serum muscle enzymes among different types of myopathies and viral hepatitis. Methods This study enrolled 578 patients with myopathies and 51 patients with viral hepatitis from January to September 2018. Serum muscle enzymes [creatine kinase (CK), lactic dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and AST/ALT ratio] were detected in all patients, and the expression differences of serum muscle enzymes were compared among different types of myopathies, and between myopathies and viral hepatitis. Results in myopathy patients, there were significant differences on the values of CK (χ² = 107.710, P = 0.000), LDH (χ² = 36.832, P = 0.000), AST (χ² = 47.608, P = 0.000) and ALT (χ² = 31.722, P = 0.000) among different types of myopathies. Patients with muscular dystrophy had significantly higher expression of CK and LDH than inflammatory myopathy (Z = 9.297, P = 0.000; Z = 2.475, P = 0.040) and metabolic myopathy (Z = 6.765, P = 0.000; Z = 5.983, P = 0.000), higher expression of AST than metabolic myopathy (Z = - 5.363, P = 0.000), and higher ALT than inflammatory myopathy (Z = 5.619, P = 0.000). The value of LDH in metabolic myopathy patients was significantly higher than that in inflammatory myopathy (Z = 4.590, P = 0.000). Compared with patients with viral hepatitis, myopathy patients had significantly increased CK (Z = - 10.975, P = 0.000), LDH (Z = - 6.967, P = 0.000) and AST/ALT ratio (Z = - 5.605, P = 0.000), and decreased ALT (Z = 3.949, P = 0.000). Receiver operating characteristic (ROC) curve showed the area under the curve (AUC) of CK, LDH, AST, ALT and AST/ALT ratio were 0.943 (95% CI: 0.919-0.966, P = 0.000), 0.818 (95%CI: 0.756-0.881, P = 0.000), 0.446 (95%CI: 0.350-0.542, P = 0.217), 0.323 (95% CI: 0.224-0.422, P = 0.000), 0.745 (95% CI: 0.671-0.819, P = 0.000), respectively. In the differential diagnostic test between myopathies and viral hepatitis, the sensitivity of CK and ratio of AST/ ALT was 0.880 and 0.512, the specificity of which was 0.961 and 0.882, the maximum Youden index of which was 0.841 and 0.394. Conclusions The serum muscle enzymes spectrum in different types of myopathies were different, but there existed some rules, which could provide clues for clinical classification of myopathies. The ratio of AST/ALT had suggestive values in the differential diagnoses between myopathies and viral hepatitis. For patients with increased transaminase of unknown reasons, serum CK should be detected. [ABSTRACT FROM AUTHOR]

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المؤلفون: Srinivas Indu Kumari, Archana Panyala, Paramjit Grover, Srinivas Chinde

المصدر: Journal of Applied Toxicology. 42:1091-1093

مصطلحات موضوعية: Biodistribution, Administration, Oral, Aspartate transaminase, 02 engineering and technology, 010501 environmental sciences, Pharmacology, medicine.disease_cause, Toxicology, 01 natural sciences, Superoxide dismutase, chemistry.chemical_compound, Lactate dehydrogenase, medicine, Animals, Tissue Distribution, Yttrium, Particle Size, Rats, Wistar, Micronuclei, Chromosome-Defective, 0105 earth and related environmental sciences, Micronucleus Tests, biology, 021001 nanoscience & nanotechnology, Biochemistry, Alanine transaminase, chemistry, Organ Specificity, biology.protein, Nanoparticles, Alkaline phosphatase, Female, Comet Assay, 0210 nano-technology, Micronucleus, Genotoxicity, DNA Damage, Mutagens

الوصف: The increasing use of yttrium oxide (Y2O3) nanoparticles (NPs) entails an improved understanding of their potential impact on the environmental and human health. In the present study, the acute oral toxicity of Y2O3 NPs and their microparticles (MPs) was carried out in female albino Wistar rats with 250, 500 and 1000 mg kg−1 body weight doses. Before the genotoxicity evaluation, characterization of the particles by transmission electron microscopy, dynamic light scattering and laser Doppler velocimetry was performed. The genotoxicity studies were conducted using micronucleus and comet assays. Results showed that Y2O3 NP-induced significant DNA damage at higher dose (1000 mg kg−1 body weight) in peripheral blood leukocytes and liver cells, micronucleus formation in bone marrow and peripheral blood cells. The findings from biochemical assays depicted significant alterations in aspartate transaminase, alanine transaminase, alkaline phosphatase, malondialdehyde, superoxide dismutase, reduced glutathione, catalase and lactate dehydrogenase levels in serum, liver and kidneys at the higher dose only. Furthermore, tissue biodistribution of both particles was analyzed by inductively coupled plasma optical emission spectrometry. Bioaccumulation of yttrium (Y) in all tissues was significant and dose-, time- and organ-dependent. Moreover, Y2O3 NP-treated rats exhibited higher tissue distribution along with greater clearance through urine whereas Y2O3 MP-dosed animals depicted the maximum amount of Y in the feces. Hence, the results indicated that bioaccumulation of Y2O3 NPs via its Y ions may induce genotoxic effects.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c8314324d94e658ff16fe6893a20d96Test
https://doi.org/10.1002/jat.4326Test

المؤلفون: Jianhua Hu, Yanggan Wang

المصدر: Gerontology

مصطلحات موضوعية: Male, Aging, Disease, Comorbidity, Gastroenterology, Risk Factors, Troponin I, medicine.diagnostic_test, Troponin T, Coronavirus disease 2019, 05 social sciences, Age Factors, Alanine Transaminase, Middle Aged, C-Reactive Protein, Cardiovascular Diseases, Creatinine, 2019-nCoV, Hypertension, Severe acute respiratory syndrome-CoV-2, Female, 0305 other medical science, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), 050105 experimental psychology, 03 medical and health sciences, Age Distribution, 030502 gerontology, Diabetes mellitus, Internal medicine, Lymphopenia, medicine, Diabetes Mellitus, Increased lactate dehydrogenase, Blood test, Humans, 0501 psychology and cognitive sciences, Aspartate Aminotransferases, Obesity, Sex Distribution, Aged, Clinical Section: Meta-Analysis, Pandemic, L-Lactate Dehydrogenase, business.industry, SARS-CoV-2, Albumin, COVID-19, medicine.disease, Coronavirus, Geriatrics and Gerontology, business

الوصف: Objective: We aim to investigate the clinical characteristics and risk factors for the severe cases of coronavirus disease 2019 (COVID-19) in comparison with the non-severe patients. Methods: We searched PubMed, EMBASE, Web of Science, and CNKI to collect all relevant studies published before July 26, 2020, and a total of 30 papers were included in this meta-analysis. Results: In the severe COVID-19 patients, 60% (95% CI = 56–64%) were male, 25% (95% CI = 21–29%) were over 65 years old, 34% (95% CI = 24–44%) were obese, and 55% (95% CI = 41–70%) had comorbidities. The most prevalent comorbidities were hypertension (34%, 95% CI = 25–44%), diabetes (20%, 95% CI = 15–25%), and cardiovascular disease (CVD; 12%, 95% CI = 9–16%). The most common blood test abnormalities were elevated C-reactive protein (CRP; 87%, 82–92%), decreased lymphocyte count (68%, 58–77%), and increased lactate dehydrogenase (69%, 95% CI = 57–81%). In addition, abnormal laboratory findings revealing organ dysfunctions were frequently observed in the severe cases, including decrease in albumin (43%, 95% CI = 24–63%) and increase in aspartate aminotransferase (47%, 95% CI = 38–56%), alanine aminotransferase (28%, 95% CI = 16–39%), troponin I/troponin T (TnI/TnT; 29%, 95% CI = 13–45%), and serum Cr (SCr; 10%, 95% CI = 5–15%). Conclusion: The male, elderly and obese patients and those with any comorbidities, especially with hypertension, diabetes, and CVD, were more likely to develop into severe cases. But the association between hypertension, diabetes, CVD, and severity of COVID-19 was declined by the increase of age. A significant elevation in cardiac TnI/TnT, the hepatic enzymes, and SCr and the reduction in lymphocytes with elevated CRPs are important markers for the severity. Specific attention should be given to the elderly male and obese patients and those with indications of severe immune injury in combination with bacterial infection and indication of multi-organ dysfunction or damages.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67675ee9df2298861c3e0442cc2903f7Test
http://europepmc.org/articles/PMC7900480Test

المؤلفون: Guosheng Gao, Ting Cai, Yi Sun

المصدر: Bioengineered, Vol 12, Iss 1, Pp 6459-6471 (2021)
Bioengineered
article-version (VoR) Version of Record

مصطلحات موضوعية: Bioengineering, Dehydrogenase, stabilizer, medicine.disease_cause, Applied Microbiology and Biotechnology, Bacterial Proteins, Enzyme Stability, medicine, Humans, Asparagine, Lactate Dehydrogenases, Escherichia coli, chemistry.chemical_classification, Lactobacillus delbrueckii, biology, Alanine Transaminase, enzymatic characterization, General Medicine, Recombinant Proteins, Enzyme assay, Amino acid, d-lactate dehydrogenase, Enzyme, chemistry, Biochemistry, D-lactate dehydrogenase, biology.protein, Specific activity, alt kit, Blood Chemical Analysis, TP248.13-248.65, Research Article, Research Paper, Biotechnology

الوصف: D-lactate dehydrogenase (D-LDH) is widely used for the clinical detection of alanine aminotransferase (ALT) activity. It is a key enzyme in ALT detection kits, and its enzymatic properties directly determine sensitivity and accuracy of such kits. In this study, D-lactate dehydrogenase (WP_011543503, ldLDH) coding sequence derived from Lactobacillus delbrueckii was obtained from the NCBI database by gene mining. LdLDH was expressed and purified in Escherichia coli, and its enzyme activity, kinetic parameters, optimum temperature, and pH were characterized. Furthermore, stabilizers, including sugars, polyols, amino acids, certain salts, proteins, and polymers, were screened to improve stability of ldLDH during freeze-drying and storage. Finally, a kit based on ldLDH was tested to determine whether the enzyme had potential clinical applications. The results showed that ldLDH had a specific activity of 1,864 U/mg, Km value of 1.34 mM, optimal reaction temperature of 55°C, and an optimal pH between 7.0 and 7.5. When sucrose or asparagine was used as a stabilizer, freeze-dried ldLDH remained stable at 37°C for > 2 months without significant loss of enzymatic activity. These results indicated that ldLDH possesses high activity and stability. Test results using the ALT assay kit prepared with ldLDH were consistent with those of commercial kits, with a relative deviation

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddcd2393b47e1b9415d146692bd87afaTest
https://doi.org/10.1080/21655979.2021.1972781Test

المؤلفون: Zainul Amiruddin Zakaria, Md. Atiar Rahman, Salama Mostafa Aboelenin, Md. Nazim Uddin, Afnan M. Alnajeebi, Nouf Abubakr Babteen

المصدر: BioMed Research International, Vol 2021 (2021)
BioMed Research International

مصطلحات موضوعية: Male, Article Subject, Blood sugar, Network Pharmacology, Pharmacology, Antioxidants, General Biochemistry, Genetics and Molecular Biology, Diabetes Mellitus, Experimental, Superoxide dismutase, chemistry.chemical_compound, Blood serum, In vivo, Lactate dehydrogenase, Animals, Hypoglycemic Agents, Aspartate Aminotransferases, Rats, Wistar, Oleanolic acid, chemistry.chemical_classification, Glutathione Peroxidase, General Immunology and Microbiology, biology, Plant Extracts, Superoxide Dismutase, Chemistry, Glutathione peroxidase, Alanine Transaminase, General Medicine, NFE2L2, Rats, Oxidative Stress, Liver, Vitaceae, biology.protein, Medicine, Medicine, Traditional, Research Article

الوصف: Background. Hatikana is a traditional medicinal plant used to treat inflammation, urolithiasis, goiter, cancer, wounds and sores, gastrointestinal, tumor, tetanus, arthritis, hepatic damage, neurodegeneration, and other ailments. The goal of this study is to investigate the antidiabetic properties of Hatikana extract (HKEx) and to construct the effects of its natural constituents on the genes and biochemical indices that are connected with them. Methods. HKEx was evaluated using GC-MS and undertaken for a three-week intervention in fructose-fed STZ-induced Wistar albino rats at the doses of HKEx50, HKEx100, and HKEx200 mg/kg bw. Following intervention, blood serum was examined for biochemical markers, and liver tissue was investigated for the mRNA expression of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD1) by RTPCR analysis. Most abundant compounds (oleanolic acid, 7α, 28-olean diol, and stigmasterol) from GC-MS were chosen for the network pharmacological assay to verify function-specific gene-compound interactions using STITCH, STRING, GSEA, and Cytoscape plugin cytoHubba. Results. In vivo results showed a significant ( P < 0.05 ) decrease of blood sugar, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine kinase (CK-MB), and lactate dehydrogenase (LDH) and increase of liver glycogen, glucose load, and serum insulin. Out of three antioxidative genes, catalase (CAT) and superoxide dismutase (SOD1) were found to be few fold increased. Oleanolic acid and stigmasterol were noticed to strongly interact with 27 target proteins. Oleanolic acid interacted with the proteins AKR1B10, CASP3, CASP8, CYP1A2, CYP1A2, HMGB1, NAMPT, NFE2L2, NQO1, PPARA, PTGIR, TOP1, TOP2A, UGT2B10, and UGT2B11 and stigmasterol with ABCA1, ABCG5, ABCG8, CTSE, HMGCR, IL10, CXCL8, NR1H2, NR1H3, SLCO1B1, SREBF2, and TNF. Protein-protein interaction (PPI) analysis revealed the involvement of 25 target proteins out of twenty seven. Cytoscape plugin cytoHubba identified TNF, CXCL8, CASP3, PPARA, SREBF2, and IL10 as top hub genes. Pathway analysis identified 31 KEGG metabolic, signaling, and immunogenic pathways associated with diabetes. Notable degree of PPI enrichment showed that SOD1 and CAT are responsible for controlling signaling networks and enriched pathways. Conclusion. The findings show that antioxidative genes have regulatory potential, allowing the HKEx to be employed as a possible antidiabetic source pending further validation.

وصف الملف: text/xhtml

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::171b1443e18d6abbdae6428f61004962Test
https://doi.org/10.1155/2021/6978450Test

المؤلفون: Ikhlas Sindi, Mohamed El Yamani, May Ahmed Galal, Sherifa Kamal, Mohammed Fouad Shalaby, Hekma A. Abd El Latif, Raneem Masaood

المصدر: BMC Pharmacology and Toxicology, Vol 22, Iss 1, Pp 1-9 (2021)
BMC Pharmacology & Toxicology

مصطلحات موضوعية: Blood Glucose, Male, Myocardial Infarction, Sarpogrelate, Diabetic nephropathy, Kidney, Cardiovascular, chemistry.chemical_compound, RA1190-1270, Medicine, Diabetic Nephropathies, Pharmacology (medical), Dopamine receptors, Blood urea nitrogen, biology, Isoenzymes, Myocardial injury, Dopamine Agonists, Serotonin 5-HT2 Receptor Antagonists, Drug Therapy, Combination, Research Article, medicine.drug, medicine.medical_specialty, Cabergoline, Aspartate transaminase, RM1-950, Diabetes Mellitus, Experimental, Internal medicine, Dopamine receptor D2, Diabetes mellitus, Animals, Rats, Wistar, Bromocriptine, Pharmacology, L-Lactate Dehydrogenase, Tumor Necrosis Factor-alpha, business.industry, Myocardium, Troponin I, Succinates, medicine.disease, TNFα1, Endocrinology, Alanine transaminase, chemistry, Toxicology. Poisons, biology.protein, Therapeutics. Pharmacology, business

الوصف: Background Dopamine D2 receptor agonists, bromocriptine and cabergoline, are notable medications in the treatment of Parkinsonism, hyperprolactinemia, and hyperglycemia. An affiliation was found between the initiation of myocardial injury ailment and long term treatment with dopamine D2 agonist drugs identified with the partial activation of 5-hydroxytryptamine receptor 2 A (5-HT2A). The investigation aimed to examine the activity of sarpogrelate (a 5-HT2A receptor blocker) in reducing myocardial injury prompted by extended haul utilisation of D2 receptor agonists in rats with alloxan-induced diabetes. Methods Both bromocriptine and cabergoline were managed independently and combined with sarpogrelate for about a month in diabetic nephropathy rats. Both tail-cuff blood pressure and the BGL were recorded weekly. For all animals, the kidney hypertrophy index, serum creatinine, blood urea nitrogen, alanine transaminase, and aspartate transaminase levels were measured after one month of treatment. The severity of the cardiac injury was assessed by the estimation of lactate dehydrogenase-1 (LDH-1), cardiac troponin I, and tumor necrosis factor alpha 1 (TNF1). The triphenyltetrazolium chloride (TTC) staining method was used to determine the experimental myocardial infarction (MI) size. Results Bromocriptine and cabergoline created a significant reduction in BGL, BP, and kidney hypertrophy index in diabetic nephropathy rats. Administration of bromocriptine and cabergoline, alone, or in combination with sarpogrelate fundamentally diminished the blood concentrations of alkaline phosphatase (ALP), Aspartate aminotransferase (AST), urea, and creatinine. Bromocriptine and cabergoline alone showed a noteworthy increase in the LDH-1, Troponin I, and TNF1 levels in the serum (p Conclusions The examination demonstrated that the combined use of sarpogrelate with bromocriptine or cabergoline decreased the potential adverse effects of these two drugs on the myocardial tissues.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aedea16e4f3fe596c1c4068e954e006dTest
https://doaj.org/article/7d4cd74b37474dee8e89e85b0d38c33fTest

المؤلفون: Jin Tae Kim, Ho Jin Park, Ji Hyun Moon, Shuai Qiu, Seung Beom Lee, Hong Jin Lee, Yimeng Zhou

المصدر: Journal of Microbiology and Biotechnology. 31:1256-1261

مصطلحات موضوعية: Antioxidant, biology, Chemistry, medicine.medical_treatment, Rubus coreanus, Aspartate transaminase, General Medicine, Glutathione, Pharmacology, biology.organism_classification, medicine.disease_cause, Applied Microbiology and Biotechnology, chemistry.chemical_compound, Alanine transaminase, Black raspberry, Lactate dehydrogenase, biology.protein, medicine, Oxidative stress, Biotechnology

الوصف: Rubus coreanus Miquel (bokbunja), Korean black raspberry, is known to possess various phytochemicals that exert antioxidative, anti-inflammatory, and anti-cancer effects. However, most studies on Rubus coreanus Miquel have been performed with the solvent extracts and/or a single component to demonstrate the efficacy, while studies evaluating the effect of the whole fructus of Rubus coreanus Miquel are limited. In this study, therefore, we employed the isoproterenol (IPN)-induced myocardial infarction model and investigated the effect of freeze-dried powder of Rubus coreanus Miquel (RCP) on oxidative stress and prevention of organ damage. Oral administration of RCP reduced the level of toxicity markers, alanine transaminase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) without affecting body weight and diet intake. The oxidative stress marker glutathione (GSH) increased about 45% and malonaldehyde (MDA) decreased about 27% compared to the IPN group with RCP-H (3%) administration. By histological analysis, IPN induced significant myocardial damage in the heart and vascular injury in the liver, and RCP administration ameliorated the damages in a dose-dependent manner. Taken together, RCP activated the antioxidant system leading to prevention of damage to organs by IPN in rats, making it possible to expect beneficial efficacies by consuming the whole fructus of Rubus coreanus Miquel.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::38658064462046ee4fb029b38ead8161Test
https://doi.org/10.4014/jmb.2106.06047Test