Transcriptome remodeling of differentiated cells during chronological ageing of yeast colonies: New insights into metabolic differentiation
| العنوان: | Transcriptome remodeling of differentiated cells during chronological ageing of yeast colonies: New insights into metabolic differentiation |
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| المؤلفون: | Otakar Hlaváček, Libuše Váchová, Ragnhild Aaløkken, Jana Maršíková, Gregor D. Gilfillan, Eva Ježková, Zdena Palková, Derek Wilkinson |
| المصدر: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2018 (2018) |
| بيانات النشر: | Hindawi, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Aging, Cell type, Saccharomyces cerevisiae Proteins, Article Subject, Cellular differentiation, Population, Cell, Saccharomyces cerevisiae, Biology, Mitochondrion, Biochemistry, Transcriptome, 03 medical and health sciences, medicine, lcsh:QH573-671, education, education.field_of_study, lcsh:Cytology, Gene Expression Profiling, Cell Biology, General Medicine, Yeast, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Ageing, Research Article |
| الوصف: | We present the spatiotemporal metabolic differentiation of yeast cell subpopulations from upper, lower, and margin regions of colonies of different ages, based on comprehensive transcriptomic analysis. Furthermore, the analysis was extended to include smaller cell subpopulations identified previously by microscopy within fully differentiated U and L cells of aged colonies. New data from RNA-seq provides both spatial and temporal information on cell metabolic reprogramming during colony ageing and shows that cells at marginal positions are similar to upper cells, but both these cell types are metabolically distinct from cells localized to lower colony regions. As colonies age, dramatic metabolic reprogramming occurs in cells of upper regions, while changes in margin and lower cells are less prominent. Interestingly, whereas clear expression differences were identified between two L cell subpopulations, U cells (which adopt metabolic profiles, similar to those of tumor cells) form a more homogeneous cell population. The data identified crucial metabolic reprogramming events that arise de novo during colony ageing and are linked to U and L cell colony differentiation and support a role for mitochondria in this differentiation process. |
| وصف الملف: | text/xhtml |
| تدمد: | 1942-0900 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c8f5cb741ab45eaf18e46b6f7115f61Test http://hdl.handle.net/10852/67522Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8c8f5cb741ab45eaf18e46b6f7115f61 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19420900 |
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