دورية أكاديمية
Correlation between the DNA methylation and gene expression of IGFBP5 in breast cancer
| العنوان: | Correlation between the DNA methylation and gene expression of IGFBP5 in breast cancer |
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| المؤلفون: | Karabulut S., Kaya Z., Amuran G.G., Peker I., Özmen T., Gulluolu B.M., Kaya H., Erzik C., Ozer A., Akkiprik M. |
| المساهمون: | Bayburt University, 55771646300, 55876457500, 56960629200, 56448719400, 7801655696, 57192895317, 7102340078, 23481935200, 7005139386, 57195257114 |
| بيانات النشر: | IOS Press |
| المجموعة: | Bayburt University Institutional Repository (DSpace@Bayburt) |
| مصطلحات موضوعية: | breast cancer, IGFBP5, methylation, MSP, promoter, beta actin, epidermal growth factor receptor 2, estrogen receptor, Ki 67 antigen, somatomedin binding protein 5, ERBB2 protein, human, messenger RNA, progesterone receptor, adult, age distribution, aged, Article, binding site, cancer grading, cancer prognosis, controlled study, CpG island, DNA methylation, female, gene expression regulation, gene sequence, histopathology, human tissue, lymph node metastasis |
| الوصف: | BACKGROUND: The insulin-like growth factor binding protein5 (IGFBP5) is often dysregulated in human cancers and considered neither a tumor suppressor nor an oncogene. OBJECTIVE: We aim to examine the reason of the changeable gene regulation of IGFBP5 in the case of methylation in breast cancer. METHODS: We used methyl-specific polymerase (MSP) chain reaction to detect CpG methylation of IGFBP5 promoter and exon-I in breast cancer and adjacent tissues. Gene expression is evaluated by quantative polymerase chain reaction (qPCR). RESULTS: IGFBP5 methylation was detected in 24 of 58 (41%) and 54 of 56 (96.5%) promoter and exon-I site respectively in tumor tissues. In adjacent tissues 17 of 58 (29%) and 53 of 56 (96.5%) was methylated. IGFBP5 expression was higher estrogene receptor (ER)(+) than ER(-) patients (p = 0:0549). Beside, we found a positive correlation between the expression of IGFBP5 and G2 tumor grade (p = 0:0131). However, no correlation was observed between IGFBP5 expression and age, menopause or the presence of lymph node metastasis (p > 0:05). CONCLUSIONS: In summary, our results showed that IGFBP5 promoter and exon-I methylation did not have any differences between tumor and adjacent tissues so that IGFBP5 methylation did not change IGFBP5 gene regulation in breast cancer. This is the first study investigating the IGFBP5 gene methylation in breast cancer. © 2016 - IOS Press and the authors. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0888-6008 |
| العلاقة: | Breast Disease; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://dx.doi.org/10.3233/BD-160234Test; https://hdl.handle.net/20.500.12403/668Test; 36; 123; 131 |
| DOI: | 10.3233/BD-160234 |
| الإتاحة: | https://doi.org/20.500.12403/668Test https://doi.org/10.3233/BD-160234Test https://hdl.handle.net/20.500.12403/668Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.E7D6391 |
| قاعدة البيانات: | BASE |
| تدمد: | 08886008 |
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| DOI: | 10.3233/BD-160234 |