المؤلفون: Chanida Fongsaran, Atichat Kuadkitkan, Duncan R. Smith, Nitwara Wikan

المصدر: Virology. 406(1):149-161

مصطلحات موضوعية: Aedes albopictus, Immunoprecipitation, viruses, Aedes aegypti, Biology, Dengue virus, medicine.disease_cause, Virus Replication, Virus, Dengue fever, Cell Line, Dengue, Viral Envelope Proteins, Aedes, Virology, Prohibitins, medicine, Animals, Prohibitin, RNA, Small Interfering, fungi, Dengue Virus, Virus Internalization, biology.organism_classification, medicine.disease, Cell biology, Repressor Proteins, Culex, Gene Knockdown Techniques, Host-Pathogen Interactions, biology.protein, Insect Proteins, Receptors, Virus, Antibody, Receptor

الوصف: Dengue is transmitted primarily by mosquitoes of the Aedes genus. Despite a number of studies, no insect dengue virus receptor protein has been clearly identified and characterized. Using a number of separation methodologies and virus overlay protein binding assays we identified a 35 kDa protein that segregated with susceptibility to dengue serotype 2 (DENV-2) infection in two mosquito species and two mosquito cell lines. Mass spectroscopy identified the protein to be prohibitin, a strongly conserved and ubiquitously expressed protein in eukaryotic cells. Antibody mediated inhibition of infection and siRNA mediated knockdown of prohibitin expression significantly reduced infection levels and subsequent virus production in both Aedes aegypti and Aedes albopictus cell lines. Confocal microscopy showed a significant degree of intracellular colocalization between prohibitin and DENV-2 E protein, and coimmunoprecipitation confirmed that prohibitin interacts with dengue E. Prohibitin is the first characterized insect cell expressed dengue virus receptor protein.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fc09b6d1f117cccf77a4c7a63ceac1dTest

المؤلفون: Duncan R. Smith, Nitwara Wikan, Atefeh Khakpoor, Mingkwan Panyasrivanit

المصدر: Autophagy. 5(3)

مصطلحات موضوعية: Endosome, Endocytic cycle, Endosomes, Dengue virus, Endocytosis, medicine.disease_cause, Virus Replication, Clathrin, Dengue fever, Aedes, Phagosomes, medicine, Autophagy, Animals, Humans, RNA Viruses, Antibody-dependent enhancement, Cell Lineage, Molecular Biology, biology, Cell Membrane, Cell Biology, Dengue Virus, medicine.disease, Virology, Cell biology, Viral replication, Protein Biosynthesis, biology.protein, Lysosomes

الوصف: Amphisomes are preautolysosomal vacuoles formed upon the fusion of autophagosomes with endosomes, and as such represent a critical meeting point between endocytic and autophagic pathways. Dengue virus enters into susceptible cells by clathrin-mediated endocytosis, and colocalization of dengue markers with markers of both autophagic and endosomal vesicles demonstrates that amphisomes are a site of dengue virus replication and translation. This work links for the first time the processes of dengue virus entry and translation/replication, and allows for interpretation of the early part of the dengue virus life cycle in terms of a continual association with membranes of an endosomal-autophagosomal lineage.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::644abbdfc90a0cddebf616aa066fa69dTest
https://pubmed.ncbi.nlm.nih.gov/19202356Test