دورية أكاديمية
Size Exclusion Chromatography: An Experiment for High School and Community College Chemistry and Biotechnology Laboratory Programs
| العنوان: | Size Exclusion Chromatography: An Experiment for High School and Community College Chemistry and Biotechnology Laboratory Programs |
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| اللغة: | English |
| المؤلفون: | Brunauer, Linda S., Davis, Kathryn K. |
| المصدر: | Journal of Chemical Education. May 2008 85(5):683-685. |
| الإتاحة: | Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail: jchemed@egpp.com; Web site: http://www.jce.divched.orgTest |
| تمت مراجعته من قبل الزملاء: | Y |
| وصف مادي: | |
| Page Count: | 3 |
| تاريخ النشر: | 2008 |
| نوع الوثيقة: | Journal Articles Reports - Descriptive |
| Education Level: | High Schools Two Year Colleges |
| الواصفات: | Advanced Placement, Chemistry, Laboratories, Biotechnology, Biology, Biochemistry, Educational Environment, Science Instruction, College Science, High Schools, Comparative Analysis, Animals |
| تدمد: | 0021-9584 |
| مستخلص: | A simple multiday laboratory exercise suitable for use in a high school or community college chemistry course or a biotechnology advanced placement biology course is described. In this experiment students gain experience in the use of column chromatography as a tool for the separation and characterization of biomolecules, thus expanding their exposure to chromatographic procedures beyond those more commonly employed in the high school environment (e.g., paper or thin-layer chromatography). Students chromatographically separate a standard mixture of three highly colored compounds of varying molar mass (vitamin B[subscript 12], bovine hemoglobin, and blue dextran) on a short Sephacryl S-300 size exclusion column, determining their relative elution volumes. Students later apply a sample of the enzyme wheat germ acid phosphatase to the same column, collect eluant fractions and determine the elution position of the enzyme by use of a rapid and simple enzyme assay carried out in a 96 well plate. The relative molar mass of the enzyme is estimated by comparison of its elution volume to the elution profile of the molecules in the standard mixture. (Contains 2 figures.) |
| Abstractor: | As Provided |
| Number of References: | 15 |
| Entry Date: | 2009 |
| الوصول الحر: | http://jchemed.chem.wisc.edu/Journal/Issues/2008/May/abs683.htmlTest |
| رقم الانضمام: | EJ826721 |
| قاعدة البيانات: | ERIC |
| تدمد: | 0021-9584 |
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