المؤلفون: Kenneth I. Ataga, Emily J Ciccone, Jianwen Cai, Vimal K. Derebail, Qingning Zhou

المصدر: Br J Haematol

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Urology, Renal function, Angiotensin-Converting Enzyme Inhibitors, Anemia, Sickle Cell, Kaplan-Meier Estimate, Kidney, Article, Sickle Cell Trait, Young Adult, chemistry.chemical_compound, Interquartile range, medicine, Humans, Antihypertensive Agents, Proportional Hazards Models, Retrospective Studies, Creatinine, Proteinuria, business.industry, beta-Thalassemia, Hazard ratio, Hematology, Odds ratio, Confidence interval, chemistry, Hypertension, Female, Kidney Diseases, medicine.symptom, business, Angiotensin II Type 1 Receptor Blockers, Glomerular hyperfiltration, Follow-Up Studies, Glomerular Filtration Rate

الوصف: Glomerular hyperfiltration is common in sickle cell disease (SCD) and precedes proteinuria and declining kidney function. We evaluated hyperfiltration in SCD patients and its "normalization." Routine visit data were collected retrospectively from adult SCD patients in a single centre from 2004 to 2013. Baseline was defined as first available serum creatinine and hyperfiltration as estimated glomerular filtration rates (eGFR) >130 ml/min/1·73 m2 for women and >140 ml/min/1·73 m2 for men. Normalization of hyperfiltration was eGFR reduction to 90-130 ml/min/1·73 m2 for women or 90-140 ml/min/1·73 m2 for men. Among 292 patients, median age was 27 years [interquartile range (IQR):20·0-38·0], and 56·8% had baseline hyperfiltration. Baseline hyperfiltration was inversely associated with age [odds ratio (OR):0·86, 95% confidence interval (CI): 0·82-0·90; P < 0·0001], male sex (OR:0·16, 95% CI: 0·07-0·41; P = 0·0001), haemoglobin (OR:0·76, 95% CI 0·61-0·94; P = 0·01), weight (OR:0·96, 95% CI: 0·93-0·99; P = 0·004), and angiotensin-converting enzyme inhibitor/angiotensin receptor blocker (ACE-I/ARB) use (OR:0·08, 95% CI: 0·01-0·75; P = 0·03), and positively with hydroxycarbamide use (OR:2·99, 95% CI: 1·18-7·56; P = 0·02). Of 89 hyperfiltration patients without baseline proteinuria, 10 (11·2%) developed new-onset proteinuria [median 1·05 years (IQR:0·63-2·09)]. Normalization of hyperfiltration was less likely with higher baseline eGFR [hazard ratio (HR):0·90, 95% CI: 0·86-0·95; P < 0·0001] and more likely in males (HR:6·35, 95% CI:2·71-14·86

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::656fc1fc85c8d02da6b7c2266d02290bTest
https://doi.org/10.1111/bjh.17723Test

المؤلفون: Kim C Donaghue, Bruce R. King, D Jane Holmes-Walker, Craig Jefferies, Meng Tuck Mok, P Shane Hamblin, Melissa Chee, Timothy W. Jones, Helen L. Barrett, Elizabeth E Davis, Arul Earnest, Benjamin J Wheeler, Richard O. Sinnott, Stephanie R. Johnson, Anthony Zimmermann, Glenn M. Ward, Fergus J. Cameron, P. Gerry Fegan, Jenny Couper, Maria E. Craig, Philip Bergman, Peter G. Colman

المصدر: The Journal of Clinical Endocrinology & Metabolism. 106:133-142

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Adolescent, Databases, Factual, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Overweight, Community Networks, Biochemistry, Body Mass Index, Young Adult, chemistry.chemical_compound, Endocrinology, Risk Factors, Interquartile range, Internal medicine, Diabetes mellitus, Humans, Medicine, Longitudinal Studies, Child, Creatinine, Type 1 diabetes, Australasia, business.industry, Cholesterol, Biochemistry (medical), Age Factors, medicine.disease, Diabetes Mellitus, Type 1, Blood pressure, chemistry, Cardiovascular Diseases, Heart Disease Risk Factors, Child, Preschool, Female, medicine.symptom, business, Body mass index, Diabetic Angiopathies

الوصف: Context Cardiovascular disease occurs prematurely in type 1 diabetes. The additional risk of overweight is not well characterized. Objective The primary aim was to measure the impact of body mass index (BMI) in youth with type 1 diabetes on cardiovascular risk factors. The secondary aim was to identify other determinants of cardiovascular risk. Design Observational longitudinal study of 7061 youth with type 1 diabetes followed for median 7.3 (interquartile range [IQR] 4-11) years over 41 (IQR 29-56) visits until March 2019. Setting 15 tertiary care diabetes centers in the Australasian Diabetes Data Network. Participants were aged 2 to 25 years at baseline, with at least 2 measurements of BMI and blood pressure. Main Outcome Measure Standardized systolic and diastolic blood pressure scores and non–high-density lipoprotein (HDL) cholesterol were co-primary outcomes. Urinary albumin/creatinine ratio was the secondary outcome. Results BMI z-score related independently to standardized blood pressure z- scores and non-HDL cholesterol. An increase in 1 BMI z-score related to an average increase in systolic/diastolic blood pressure of 3.8/1.4 mmHg and an increase in non-HDL cholesterol (coefficient + 0.16 mmol/L, 95% confidence interval [CI], 0.13-0.18; P Conclusions BMI had a modest independent effect on cardiovascular risk. Females and Indigenous Australians in particular had a more adverse risk profile.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::298c7db71620ae79ee854b42d6648800Test
https://doi.org/10.1210/clinem/dgaa727Test

المؤلفون: Johan Jendle, Paresh Dandona, John Xu, Nayyar Iqbal, Enrico Repetto, Markus F. Scheerer, Steven V. Edelman, Per-Henrik Groop, Fredrik Thoren, Moshe Phillip, Pieter Gillard, Chantal Mathieu

المساهمون: Pathology/molecular and cellular medicine, Diabetes Pathology & Therapy, Diabetes Clinic

المصدر: The Lancet Diabetes & Endocrinology. 8:845-854

مصطلحات موضوعية: Blood Glucose, Male, Endocrinology, Diabetes and Metabolism, Biomarkers/analysis, Glycated Hemoglobin A/analysis, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Glucosides, Insulin, 030212 general & internal medicine, Dapagliflozin, Prospective cohort study, education.field_of_study, Middle Aged, Prognosis, Diabetic Ketoacidosis/prevention & control, Insulin/therapeutic use, Creatinine, Drug Therapy, Combination, Female, medicine.symptom, Glomerular Filtration Rate, Adult, medicine.medical_specialty, Adolescent, Diabetic ketoacidosis, Albuminuria/prevention & control, Population, Diabetes Mellitus, Type 1/drug therapy, Hypoglycemic Agents/therapeutic use, 030209 endocrinology & metabolism, Placebo, Diabetic Ketoacidosis, Young Adult, 03 medical and health sciences, Double-Blind Method, Benzhydryl Compounds/therapeutic use, Internal medicine, Internal Medicine, medicine, Albuminuria, Humans, Hypoglycemic Agents, Benzhydryl Compounds, Adverse effect, education, Sodium-Glucose Transporter 2 Inhibitors, Aged, Glycated Hemoglobin, Type 1 diabetes, business.industry, Sodium-Glucose Transporter 2 Inhibitors/therapeutic use, Blood Glucose/analysis, Creatinine/blood, medicine.disease, Glucosides/therapeutic use, Diabetes Mellitus, Type 1, chemistry, business, Biomarkers, Follow-Up Studies

الوصف: Summary Background The DEPICT-1 and DEPICT-2 studies showed that dapagliflozin as an adjunct to insulin in individuals with inadequately controlled type 1 diabetes improved glycaemic control and bodyweight, without increase in risk of hypoglycaemia. We aimed to determine the effect of dapagliflozin on urinary albumin-to-creatinine ratio (UACR) and estimated glomerular filtration rate (eGFR) using pooled data from the DEPICT studies. Methods In this post-hoc analysis, we used data pooled from both DEPICT studies (DEPICT-1 ran from Nov 11, 2014, to Aug 25, 2017; DEPICT-2 ran from July 8, 2015, to April 18, 2018), in which participants were aged 18–75 years, with inadequately controlled type 1 diabetes and with a baseline UACR of at least 30 mg/g. In the DEPICT studies, participants were randomly assigned (1:1:1) to receive dapagliflozin (5 mg or 10 mg) or placebo all plus insulin, for 24 weeks, with a 28-week long-term extension (ie, 52 weeks in total). In this post-hoc analysis, we assessed the percentage change from baseline in UACR and in eGFR, up to 52 weeks. UACR, eGFR, and safety were assessed in all eligible participants who had received at least one dose of study drug. HbA1c, bodyweight, and systolic blood pressure were assessed in all participants who received at least one dose of study drug during the first 24-week period, and who had a baseline and any post-baseline assessment for that parameter. The DEPICT trials were registered with ClinicalTrials.gov , NCT02268214 (DEPICT-1), NCT02460978 (DEPICT-2), and are now complete. Results 251 participants with albuminuria at baseline were included in this post-hoc analysis; of whom 80 (32%) had been randomly assigned to dapagliflozin 5 mg, 84 (33%) to dapagliflozin 10 mg, and 87 (35%) to placebo. Compared with placebo, treatment with both dapagliflozin doses improved UACR over 52 weeks. At week 52, mean difference in change from baseline versus placebo in UACR was −13·3% (95% CI −37·2 to 19·8) for dapagliflozin 5 mg and −31·1% (−49·9 to −5·2) for dapagliflozin 10 mg. No notable change from baseline was seen in eGFR, with a mean difference in change from baseline versus placebo of 3·27 mL/min per 1·73 m2 (95% CI −0·92 to 7·45) for dapagliflozin 5 mg and 2·12 mL/min per 1·73 m2 (–2·03 to 6·27) for dapagliflozin 10 mg. Similar proportions of participants in each treatment group had adverse events and serious adverse events, including hypoglycaemia and diabetic ketoacidosis; no new safety signals were identified in this population. Interpretation Treatment with dapagliflozin resulted in UACR reduction, which might provide renoprotective benefits in individuals with type 1 diabetes and albuminuria. Dedicated prospective studies are needed to confirm these findings as prespecified endpoints. Funding AstraZeneca.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00f7846f46b1b29ed6f481ec3393eac5Test
https://doi.org/10.1016/s2213-8587Test(20)30280-1

المؤلفون: Shinae Yu, Kyo Won Lee, Hye Ryoun Jang, Ghee Young Kwon, Wooseong Huh, Eun-Suk Kang, Sung Joo Kim, Jae Berm Park, Hyung Hwan Moon, Hee Jae Huh

المصدر: Annals of Laboratory Medicine

مصطلحات موضوعية: 0301 basic medicine, Graft Rejection, Male, Clinical Biochemistry, Kaplan-Meier Estimate, 030230 surgery, Kidney, Gastroenterology, Low-risk, Kidney transplantation, chemistry.chemical_compound, 0302 clinical medicine, Risk Factors, Medicine, Outcome, biology, General Medicine, Middle Aged, Creatinine, Original Article, Female, Antibody, Glomerular Filtration Rate, Adult, medicine.medical_specialty, Anti-angiotensin II type 1 receptor antibodies, Renal function, Human leukocyte antigen, Non-HLA antibodies, Antibodies, Receptor, Angiotensin, Type 1, 03 medical and health sciences, ABO blood group system, Internal medicine, Humans, Transplantation, Homologous, Endothelial cell crossmatch, Diagnostic Immunology, Autoantibodies, Proportional Hazards Models, business.industry, Anti-endothelial cell antibodies, Biochemistry (medical), medicine.disease, Angiotensin II, Transplantation, 030104 developmental biology, chemistry, biology.protein, business

الوصف: Background Non-HLA antibodies, anti-angiotensin II type 1 receptor antibodies (anti-AT1R) and anti-endothelial cell antibodies (AECA), are known to play a role in allograft rejection. We evaluated the role of both antibodies in predicting post-transplant outcomes in low-risk living donor kidney transplantation (LDKT) recipients. Methods In 94 consecutive LDKT recipients who were ABO compatible and negative for pre-transplant HLA donor-specific antibodies, we determined the levels of anti-AT1Rs using an enzyme-linked immunosorbent assay and the presence of AECAs using a flow cytometric endothelial cell crossmatch (ECXM) assay with pre-transplant sera. Hazard ratio (HR) was calculated to predict post-transplant outcomes. Results Pre-transplant anti-AT1Rs (≥11.5 U/mL) and AECAs were observed in 36 (38.3%) and 22 recipients (23.4%), respectively; 11 recipients had both. Pre-transplant anti-AT1Rs were a significant risk factor for the development of acute rejection (AR) (HR 2.09; P=0.018), while a positive AECA status was associated with AR or microvascular inflammation only (HR 2.47; P=0.004) throughout the follow-up period. In particular, AECA (+) recipients with ≥11.5 U/mL anti-AT1Rs exhibited a significant effect on creatinine and estimated glomerular filtration rate (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0947d5451286b55fe059e9be2669d56cTest
http://europepmc.org/articles/PMC7169631Test

المؤلفون: Magnus Hillman, Jonatan Dereke, Eva O. Melin

المصدر: Biology of Sex Differences, Vol 10, Iss 1, Pp 1-9 (2019)
Biology of Sex Differences

مصطلحات موضوعية: Male, Galectin 3, lcsh:Medicine, 030204 cardiovascular system & hematology, lcsh:Physiology, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Insulin, Outpatient clinic, Galectin-3, Depression (differential diagnoses), Hypolipidemic Agents, Sex Characteristics, lcsh:QP1-981, Depression, Blood Proteins, Middle Aged, Antidepressive Agents, HDL-cholesterol, Type 1 diabetes, Female, Adult, medicine.medical_specialty, Adolescent, Galectins, sCD163, Antigens, Differentiation, Myelomonocytic, Receptors, Cell Surface, 030209 endocrinology & metabolism, Gender Studies, Young Adult, 03 medical and health sciences, Antigens, CD, Antigens, Neoplasm, Internal medicine, Diabetes mellitus, Sex differences, Biomarkers, Tumor, medicine, Humans, Antihypertensive Agents, Inflammation, Creatinine, business.industry, Research, Cholesterol, HDL, lcsh:R, Odds ratio, Anthropometry, Galectin-3 binding protein, medicine.disease, Diabetes Mellitus, Type 1, Blood pressure, chemistry, business, Biomarkers

الوصف: BackgroundGalectin-3 binding protein (Gal3BP), sCD163, galectin-3, and depression have been linked to cardiovascular disease and mortality. In patients with type 1 diabetes, female sex has also been linked to cardiovascular disease and mortality. The aim was to explore whether female sex, sCD163, galectin-3, and depression were associated with Gal3BP in patients with type 1 diabetes. We adjusted for metabolic variables, creatinine, smoking, physical inactivity, and cardiovascular disease.MethodsCross-sectional design. Patients with type 1 diabetes (n = 285, women 44%, age18–59 years, diabetes duration 1–55 years) were consecutively recruited from one diabetes outpatient clinic. Blood samples, anthropometrics, and blood pressure were collected, supplemented with data from electronic medical records. High Gal3BP was defined as ≥3.3 mg/l (≥80th percentile). Depression was assessed by a self-report instrument. Linear and logistic regression models were elaborated for the associations and calibrated and validated for goodness of fit with the data variables.ResultsMedian (q1, q3) Gal3BP was 2.3 (1.8, 3.1) mg/l. The prevalence of high Gal3BP for women was 30% and 14% for men (p = 0.001). Female sex (adjusted odds ratio (AOR) 3.0), sCD163 (per μg/l) (AOR 6.6), and total cholesterol (per mmol/l) (AOR 1.6) were positively associated with high Gal3BP, and HDL-cholesterol (per mmol/l) (AOR 0.2) was negatively associated with high Gal3BP.ConclusionsHigh Gal3BP levels were associated with female sex, increasing sCD163 and total cholesterol levels, and decreasing HDL-cholesterol levels in patients with type 1 diabetes. The prevalence of high Gal3BP was more than twice as high in the women as in the men.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e2051cdf8660c701392937d89c9f476Test
http://link.springer.com/article/10.1186/s13293-019-0268-0Test

المؤلفون: Graham D. Ogle, Maria José Castañeda-Saldivar, Angela C Middlehurst, Maria Francisa Flores, Hannah Elizabeth Bartley, Norma Daniela Hernández-Pérez, Arely Gutiérrez Lara, María Elena Mota-Oropeza, Mariana Marroquín Velázquez, Nancy Alejandra Vázquez-López, Alicia J. Jenkins

المصدر: Journal of Pediatric Endocrinology and Metabolism. 32:1139-1146

مصطلحات موضوعية: Adult, Blood Glucose, Male, Several Times Daily, medicine.medical_specialty, Adolescent, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, 030209 endocrinology & metabolism, Type 2 diabetes, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Patient-Centered Care, Diabetes mellitus, Internal medicine, medicine, Humans, Hypoglycemic Agents, Glucose test, 030212 general & internal medicine, Child, Mexico, Glycated Hemoglobin, Type 1 diabetes, Creatinine, medicine.diagnostic_test, business.industry, Insulin, Infant, Newborn, Infant, Prognosis, medicine.disease, Regimen, Cross-Sectional Studies, Diabetes Mellitus, Type 1, Diabetes Mellitus, Type 2, Socioeconomic Factors, chemistry, Child, Preschool, Pediatrics, Perinatology and Child Health, Income, Female, business, Biomarkers, Follow-Up Studies

الوصف: Background The objective of this study was to determine the demographic and clinical features of youth supported by member associations of the Federación Mexicana de Diabetes and the Life for a Child Program (LFAC). Methods An analysis of 2017 Annual Clinical Data Sheets of 306 subjects from five Mexican centers was performed. Results Type 1 diabetes (T1D) was diagnosed in 292 subjects; 54.6% were female, with six diagnosed aged 1c (HbA1c) levels for those with duration ≥6 months were 8.7/8.4% (72/68 mmol/mol) and were higher in adolescents vs. children. Elevated body mass index SD, triglycerides (≥150 mg/dL) and non-high-density lipoprotein (HDL)-cholesterol (≥130 mg/dL) were common: 7.6%, 11.0% and 12.7% (n = 288, 218 and 180, respectively). Serum creatinine levels were normal in all tested subjects (n = 194). Conclusions Youth with diabetes in less-resourced families in Mexico are achieving reasonable glycemia. Most T1D patients use a basal bolus insulin regimen and test blood glucose several times daily. Some subjects have adverse vascular risk factor profiles. Further attention is needed to prevent chronic complications. Monogenic diabetes is very likely in some youth, and genetic testing is indicated.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c31966bcb382b32abac889c05a901827Test
https://doi.org/10.1515/jpem-2019-0363Test

المؤلفون: Corine Houehanou, Salimanou Ariyoh Amidou, Jacques Vigan, Dismand Houinato, Gwladys Nadia Gbaguidi, Philippe Lacroix

المساهمون: Neuroépidémiologie Tropicale (NET), CHU Limoges-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Laboratory of Chronic and Neurological Diseases Epidemiology (LEMACEN), University of Abomey Calavi (UAC), Service de Chirurgie Thoracique et Vasculaire - Médecine vasculaire [CHU Limoges], CHU Limoges

المصدر: BMC Nephrology, Vol 22, Iss 1, Pp 1-9 (2021)
BMC Nephrology
BMC Nephrology, BioMed Central, 2021, 22 (1), ⟨10.1186/s12882-021-02316-y⟩

مصطلحات موضوعية: Nephrology, Adult, Male, Rural Population, medicine.medical_specialty, 030232 urology & nephrology, Renal function, Logistic regression, Kidney, lcsh:RC870-923, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Risk Factors, Internal medicine, medicine, Prevalence, Humans, Benin, 030212 general & internal medicine, Abnormal kidney function, Rural area, Aged, 2. Zero hunger, Creatinine, business.industry, Associated factors, 1. No poverty, Anthropometry, Middle Aged, medicine.disease, lcsh:Diseases of the genitourinary system. Urology, Health Surveys, 3. Good health, Cross-Sectional Studies, chemistry, Cohort, Female, Kidney Diseases, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, business, Kidney disease, Research Article, Glomerular Filtration Rate

الوصف: Background The global burden of kidney disease has increased in recent years worldwide. Risk factors for kidney disease are common in Africa, but data on their prevalence are lacking. This study aims to determine the prevalence of abnormal kidney function and associated factors among participants included in the TAnve HEalth Study (TAHES) cohort in Benin. Methods This was a cross-sectional study nested within the TAHES cohort. It was carried out in 2019, among TAHES participants aged 25 years and above, living in Tanvè and Dékanmè, two villages located in southwestern Benin. Data on risk factors were collected using the World Health Organization’s STEPS questionnaire. Anthropometric measurements and capillary creatinine measurements were performed. Abnormal kidney function was defined as a low glomerular filtration rate ( Results Creatinine was measured among 1360 out of the 1583 participants in the cohort in 2019. The median age was 39 [32–53]. The prevalence of abnormal kidney function was 16.10%; 95%CI = [14.15–18.05]. The results of the multivariate logistic regression showed that the probability of abnormal kidney function increased significantly with age (adjusted OR (aOR) = 2.75; 95%CI = [1.83–4.14]), female gender (aOR = 2; 95%CI = [1.37–2.91]), hypertension (aOR = 1.54; 95%CI = [1.12–2.13]), high body mass index (aOR = 1, 56; 95%CI = [1.12–2.17]) and hyperglycemia (aOR = 2.86; 95%CI = [1.68–4.88]). Conclusion The prevalence of abnormal kidney function was high. These data should guide national authorities and help to raise community awareness of the benefits of early detection of this condition.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64719322d2d81b9e22e1b3ce6100a7e8Test
https://doaj.org/article/067f49f692d248049233e30608447894Test

المؤلفون: Lynn Ang, Subramaniam Pennathur, Anna V. Mathew, Rodica Pop-Busui, Mamta Jaiswal, George Michailidis

المصدر: Diabetes

مصطلحات موضوعية: 0301 basic medicine, Adult, Blood Glucose, Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Citric Acid Cycle, Renal function, 030209 endocrinology & metabolism, Autonomic Nervous System, Cardiovascular System, 03 medical and health sciences, chemistry.chemical_compound, Young Adult, 0302 clinical medicine, Diabetic Neuropathies, Internal medicine, Diabetes mellitus, Internal Medicine, Autonomic reflex, Medicine, Humans, Metabolomics, Amino Acids, Type 1 diabetes, Creatinine, business.industry, Cholesterol, Genetics/Genomes/Proteomics/Metabolomics, Ornithine, Middle Aged, medicine.disease, Glutamine, 030104 developmental biology, Endocrinology, Diabetes Mellitus, Type 1, chemistry, Disease Progression, Female, business

الوصف: While diabetes is characterized by hyperglycemia, nutrient metabolic pathways like amino acid and tricarboxylic acid (TCA) cycle are also profoundly perturbed. As glycemic control alone does not prevent complications, we hypothesized that these metabolic disruptions are responsible for the development and progression of diabetic cardiovascular autonomic neuropathy (CAN). We performed standardized cardiovascular autonomic reflex tests and targeted fasting plasma metabolomic analysis of amino acids and TCA cycle intermediates in subjects with type 1 diabetes and healthy control subjects followed for 3 years. Forty-seven participants with type 1 diabetes (60% female and mean ± SD age 35 ± 13 years, diabetes duration 13 ± 7 years, and HbA1c 7.9 ± 1.2%) had lower fumarate levels and higher threonine, serine, proline, asparagine, aspartic acid, phenylalanine, tyrosine, and histidine levels compared with 10 age-matched healthy control subjects. Higher baseline fumarate levels and lower baseline amino acid levels—asparagine and glutamine—correlate with CAN (lower baseline SD of normal R-R interval [SDNN]). Baseline glutamine and ornithine levels also associated with the progression of CAN (lower SDNN at 3 years) and change in SDNN, respectively, after adjustment for baseline HbA1c, blood glucose, BMI, cholesterol, urine microalbumin-to- creatinine ratio, estimated glomerular filtration rate, and years of diabetes. Therefore, significant changes in the anaplerotic flux into the TCA cycle could be the critical defect underlying CAN progression.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd8dc613f7576a877562aa275603ba2dTest
http://europepmc.org/articles/PMC6754246Test

المؤلفون: Richard J. Johnson, Janet K. Snell-Bergeon, Rachel M. Sippl, David Z.I. Cherney, Randy Wong, Marian Rewers, Petter Bjornstad, Pattara Wiromrat

المصدر: Diabetes Care. 42:297-302

مصطلحات موضوعية: Adult, Male, Cardiovascular and Metabolic Risk, medicine.medical_specialty, Time Factors, Endocrinology, Diabetes and Metabolism, Urology, Renal function, 030209 endocrinology & metabolism, Coronary Artery Disease, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Risk Factors, Diabetes mellitus, Uromodulin, Internal Medicine, medicine, Humans, Diabetic Nephropathies, 030212 general & internal medicine, Renal Insufficiency, Chronic, Vascular Calcification, Advanced and Specialized Nursing, Type 1 diabetes, Creatinine, business.industry, Case-control study, Odds ratio, Middle Aged, Atherosclerosis, Prognosis, medicine.disease, Diabetes Mellitus, Type 1, chemistry, Case-Control Studies, Disease Progression, Albuminuria, Female, medicine.symptom, Tomography, X-Ray Computed, business, Biomarkers, Follow-Up Studies, Glomerular Filtration Rate, Kidney disease

الوصف: OBJECTIVE Novel biomarkers are needed to better predict coronary artery calcification (CAC), a marker of subclinical atherosclerosis, and diabetic kidney disease (DKD) in type 1 diabetes. We evaluated the associations between serum uromodulin (SUMOD [a biomarker associated with anti-inflammatory and renal protective properties]), CAC progression, and DKD development over 12 years. RESEARCH DESIGN AND METHODS Participants (n = 527, 53% females) in the Coronary Artery Calcification in Type 1 Diabetes (CACTI) study were examined during 2002–2004, at a mean age of 39.6 ± 9.0 years and a median duration of diabetes of 24.8 years. Urine albumin-to-creatinine ratio (ACR) and estimated glomerular filtration rate (eGFR) determined by the CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) creatinine equation were measured at baseline and after a mean follow-up period of 12.1 ± 1.5 years. Elevated albumin excretion was defined as ACR ≥30 mg/g, rapid GFR decline (>3 mL/min/1.73 m2/year), and impaired GFR as eGFR RESULTS Higher baseline SUMOD level conferred lower odds of CAC progression (odds ratio 0.68; 95% CI 0.48–0.97), incident elevated albumin excretion (0.37; 0.16–0.86), rapid GFR decline (0.56; 0.35–0.91), and impaired GFR (0.44; 0.24–0.83) per 1 SD increase in SUMOD (68.44 ng/mL) after adjustment for baseline age, sex, systolic blood pressure, LDL cholesterol, and albuminuria/GFR. The addition of SUMOD to models with traditional risk factors also significantly improved the prediction performance for CAC progression and incident DKD. CONCLUSIONS Higher baseline SUMOD level predicted lower odds of both CAC progression and incident DKD over 12 years in adults with type 1 diabetes.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aba789be14020513f3ef4b000b45f6f7Test
https://doi.org/10.2337/dc18-1527Test

المؤلفون: Dânya Bandeira Lima, Geraldo Bezerra da Silva Junior, Gdayllon Cavalcante Meneses, Daniella Bezerra Duarte, Alice Maria Costa Martins, Elizabeth De Francesco Daher

المصدر: Tropical Medicine & International Health. 25:1140-1144

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Adolescent, Urinary system, 030231 tropical medicine, Schistosomiasis, Urine, Gastroenterology, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Western blot, Internal medicine, medicine, Animals, Humans, Aged, Solute Carrier Family 12, Member 1, Splenic Diseases, Creatinine, Aquaporin 2, integumentary system, biology, medicine.diagnostic_test, urogenital system, business.industry, Public Health, Environmental and Occupational Health, Schistosoma mansoni, Middle Aged, biology.organism_classification, medicine.disease, Cross-Sectional Studies, Infectious Diseases, chemistry, Case-Control Studies, Female, Kidney Diseases, Parasitology, Complication, business

الوصف: Schistosoma mansoni infection is considered a public health problem. Glomerular involvement in schistosomiasis is a well-documented complication, especially in hepatosplenic schistosomiasis (HSS). However, renal tubular function is poorly understood. The aim of this study was to investigate, through urinary exosomes, tubular transporters functionally in HSS patients.Cross-sectional study of 20 HSS patients who had isolated exosomes from urine samples. Protease inhibitor was added in the urine samples who were immediately frozen at -80 °C for further exosomes isolation. After urine had thawed, urinary exosomes were obtained using extensive vortexing, centrifugation and ultracentrifugation steps of urine. Urinary transporters expression from exosomes was evaluated by western blot, including NHE3, AQP2 and NKCC2. Charge amounts for gel electrophoresis were adjusted by urinary creatinine concentration of each patient to avoid urinary concentration bias. All protein expression of HSS patients was relative to healthy controls.The expression of aquaporin-2 (AQP2) was lower in HSS patients than in controls (46.8 ± 40.7 vs. 100 ± 70.2%, P = 0.03) and the expression of the NKCC2 co-transporter was higher (191.7 ± 248.6 vs. 100 ± 43.6%, P = 0.02).The decrease of AQP2 and the increase of NKCC2 expression in HSS patients seem to be involved with the inability of urinary concentration in these patients. These data show renal tubular abnormalities in HSS patients without manifest clinical disease.L'infection à Schistosoma mansoni est considérée comme un problème de santé publique. L'atteinte glomérulaire dans la schistosomiase est une complication bien documentée, en particulier dans la schistosomiase hépatosplénique (SH). Cependant, la fonction tubulaire rénale est mal connue. Le but de cette étude était d'étudier, par le biais d'exosomes urinaires, les transporteurs tubulaires fonctionnellement chez les patients atteints de SH. MÉTHODES: Il s'agit d'une étude transversale sur 20 patients atteints de SH qui avaient des exosomes isolés d'échantillons d'urine. Un inhibiteur de protéase a été ajouté dans les échantillons d'urine qui ont été immédiatement congelés à -80°C pour un isolement supplémentaire des exosomes. Après décongélation de l'urine, des exosomes urinaires ont été obtenus en utilisant des étapes étendues de vortex, de centrifugation et d'ultracentrifugation d'urine. L'expression des transporteurs urinaires d'exosomes a été évaluée par western blot, y compris NHE3, AQP2 et NKCC2. Les quantités de charge pour l'électrophorèse sur gel ont été ajustées par la concentration de créatinine urinaire de chaque patient pour éviter un biais de concentration urinaire. Toute expression protéique des patients atteints de SH était relative à celle de témoins sains. RÉSULTATS: L'expression de l'aquaporine-2 (AQP2) était plus faible chez les patients SH que chez les témoins (46,8 ± 40,7 vs 100 ± 70,2%, P = 0,03) et l'expression du co-transporteur NKCC2 était plus élevée (191,7 ± 248,6 vs 100 ± 43,6%, P = 0,16).La diminution de l'AQP2 et l'augmentation de l'expression de NKCC2 chez les patients SH semblent être impliquées dans l'incapacité de concentration urinaire chez ces patients. Ces données montrent des anomalies tubulaires rénales chez les patients SH sans maladie clinique manifeste.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::553aa3915676ded8bbe69e9ca1e95b8aTest
https://doi.org/10.1111/tmi.13461Test