دورية أكاديمية
Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges.
| العنوان: | Direct Detection of miR-122 in Hepatotoxicity Using Dynamic Chemical Labeling Overcomes Stability and isomiR Challenges. |
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| المؤلفون: | López-Longarela, Barbara, Morrison, Emma E, Tranter, John D, Chahman-Vos, Lianne, Léonard, Jean-François, Gautier, Jean-Charles, Laurent, Sébastien, Lartigau, Aude, Boitier, Eric, Sautier, Lucile, Carmona-Saez, Pedro, Martorell-Marugan, Jordi, Mellanby, Richard J, Pernagallo, Salvatore, Ilyine, Hugh, Rissin, David M, Duffy, David C, Dear, James W, Díaz-Mochón, Juan J |
| سنة النشر: | 2020 |
| المجموعة: | Sistema Sanitario Público de Andalucía (SSPA): Repositorio |
| مصطلحات موضوعية: | Acetaminophen, Adolescent, Adult, Animals, Biomarkers, Chemical and Drug Induced Liver Injury, Dogs, Hepatocytes, Humans, Male, MicroRNAs, Rats, Young Adult |
| الوصف: | Circulating microRNAs are biomarkers reported to be stable and translational across species. MicroRNA-122 (miR-122) is a hepatocyte-specific microRNA biomarker for drug-induced liver injury (DILI). We developed a single molecule, dynamic chemical labeling (DCL) assay to directly detect miR-122 in blood. The DCL assay specifically measured miR-122 directly from 10 μL of serum or plasma without any extraction steps, with a limit of detection of 1.32 pM that enabled the identification of DILI. Testing of 192 human serum samples showed that DCL accurately identified patients at risk of DILI after acetaminophen overdose (area under ROC curve 0.98 (95% CI; 0.96-1), P |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1520-6882 |
| العلاقة: | http://hdl.handle.net/10668/14958Test; http://hdl.handle.net/20.500.11820/6a3d708a-fc7c-4d13-939a-e1fedda53e81Test |
| DOI: | 10.1021/acs.analchem.9b05449 |
| الإتاحة: | https://doi.org/20.500.11820/6a3d708a-fc7c-4d13-939a-e1fedda53e81Test https://doi.org/10.1021/acs.analchem.9b05449Test http://hdl.handle.net/10668/14958Test https://hdl.handle.net/20.500.11820/6a3d708a-fc7c-4d13-939a-e1fedda53e81Test |
| حقوق: | open access |
| رقم الانضمام: | edsbas.3F700E9D |
| قاعدة البيانات: | BASE |
| تدمد: | 15206882 |
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| DOI: | 10.1021/acs.analchem.9b05449 |