دورية أكاديمية
Fimbriae of enterotoxigenic Escherichia coli function as a mucosal carrier for a coupled heterologous antigen
| العنوان: | Fimbriae of enterotoxigenic Escherichia coli function as a mucosal carrier for a coupled heterologous antigen |
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| المؤلفون: | Verdonck, F, De Hauwere, V, Bouckaert, B, Goddeeris, Bruno, Cox, E |
| بيانات النشر: | Elsevier science bv |
| سنة النشر: | 2005 |
| المجموعة: | KU Leuven: Lirias |
| مصطلحات موضوعية: | Administration, Oral, Animals, Antibodies, Bacterial, Cholera Toxin, Escherichia coli, Fimbriae, Immunity, Mucosal, Immunization, Immunoglobulin A, Secretory, Lymphocyte Activation, Serum Albumin, Swine |
| الوصف: | Receptor-mediated uptake of orally administered antigen can lead to an antigen-specific immune response, whereas oral administration of most other non-replicating soluble antigens results in the induction of oral tolerance. In the present study, it is shown that fimbriae purified from an F4(K88)(+) enterotoxigenic Escherichia coli strain can function as a mucosal carrier molecule for the model antigen human serum albumin (HSA). Glutaraldehyde-coupled F4/HSA conjugates were able to bind F4 receptor positive (F4R(+)) enterocytes, but not to F4R(-) enterocytes. Moreover, oral immunization of F4R(+) pigs with F4/HSA conjugates induced a HSA-specific immune response, whereas oral immunization with HSA/HSA conjugates did not. This mucosal carrier function of F4 fimbriae was improved following oral co-administration of the F4/HSA conjugates with the mucosal adjuvant cholera toxin (CT) to F4R(+) pigs, since both humoral and cellular HSA-specific responses were significantly increased. In comparison with F4R(+) pigs, the HSA-specific response was reduced following oral F4/HSA+CT immunization of F4R(-) pigs. This indicates that F4 fimbriae as mucosal carrier and CT as adjuvant synergistically improve the induction of a HSA-specific immune response following oral immunization of pigs. These results could open new perspectives in the development of vaccines against enteropathogens. ; status: published |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0168-3659 |
| العلاقة: | Journal of controlled release : official journal of the Controlled Release Society vol:104 issue:2 pages:243-58; https://lirias.kuleuven.be/handle/123456789/63798Test; http://linkinghub.elsevier.com/retrieve/pii/S0168-3659Test(05)00053-2; https://lirias.kuleuven.be/bitstream/123456789/63798/1//license.txtTest |
| الإتاحة: | https://lirias.kuleuven.be/handle/123456789/63798Test http://linkinghub.elsevier.com/retrieve/pii/S0168-3659Test(05)00053-2 https://lirias.kuleuven.be/bitstream/123456789/63798/1//license.txtTest |
| رقم الانضمام: | edsbas.46BF9516 |
| قاعدة البيانات: | BASE |
| تدمد: | 01683659 |
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