التفاصيل البيبلوغرافية
| العنوان: |
Phytochemical and biological characterization of aqueous extract of Vassobia breviflora on proliferation and viability of melanoma cells: involvement of purinergic pathway. |
| المؤلفون: |
Viana, Altevir Rossato1 (AUTHOR) rossato.viana@hotmail.com, Bottari, Nathieli Bianchin1 (AUTHOR), Oviedo, Vinícius Rodrigues2 (AUTHOR), Santos, Daniel3 (AUTHOR), Londero, James Eduardo Lago1 (AUTHOR), Schetinger, Maria Rosa Chitolina1 (AUTHOR), Flores, Erico Marlon Moraes3 (AUTHOR), Pigatto, Aline4 (AUTHOR), Schuch, André Passaglia1 (AUTHOR), Krause, Alexandre5 (AUTHOR), Krause, Luciana Maria Fontanari6 (AUTHOR) |
| المصدر: |
Journal of Toxicology & Environmental Health: Part A. 2023, Vol. 86 Issue 17, p632-652. 21p. |
| مصطلحات موضوعية: |
*PURINERGIC receptors, *PHYTOCHEMICALS, *RECEPTOR-ligand complexes, *CELL survival, *BIOACTIVE compounds, *REACTIVE oxygen species, *EXTRACELLULAR enzymes |
| مستخلص: |
Vassobia breviflora belongs to the Solanaceae family, possessing biological activity against tumor cells and is a promising alternative for therapy. The aim of this investigation was to determine the phytochemical properties V. breviflora using ESI-ToF-MS. The cytotoxic effects of this extract were examined in B16-F10 melanoma cells and the relationship if any to purinergic signaling was involved. The antioxidant activity of total phenols, (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) was analyzed, as well as production of reactive oxygen species (ROS) and nitric oxide (NO) was determined. Genotoxicity was assessed by DNA damage assay. Subsequently, the structural bioactive compounds were docked against purinoceptors P2X7 and P2Y1 receptors. The bioactive compounds found in V. breviflora were N-methyl-(2S,4 R)-trans-4-hydroxy-L-proline, calystegine B, 12-O-benzoyl- tenacigenin A and bungoside B. In vitro cytotoxicity was demonstrated at concentration ranges of 0.1–10 mg/ml, and plasmid DNA breaks only at the concentration of 10 mg/ml. V. breviflora extracts affected hydrolysis by ectoenzymes, such as ectonucleoside triphosphate diphosphohydrolase (E-NTPDase) and ectoadenosine deaminase (E-ADA) which control levels of degradation and formation of nucleosides and nucleotides. In the presence of substrates ATP, ADP, AMP and adenosine, the activities of E-NTPDase, 5´-NT or E-ADA were significantly modulated by V. breviflora. N-methyl-(2S,4 R)-trans-4-hydroxy-L-proline presented higher binding affinity (according to receptor-ligand complex estimated binding affinity as evidenced by ∆G values) to bind to both P2X7 and P2Y1purinergic receptors. Our results suggest a putative interaction of V. breviflora bioactive compounds with growth inhibitory potential in B16-F10 melanoma and suggest that may be considered as promising compounds in melanoma and cancer treatment [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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