Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation
| العنوان: | Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation |
|---|---|
| المؤلفون: | Clara Munger, Timo N. Kohler, Erin Slatery, Anna L. Ellermann, Sophie Bergmann, Christopher A. Penfold, Ioakeim Ampartzidis, Yutong Chen, Florian Hollfelder, Thorsten E. Boroviak |
| المساهمون: | Kohler, Timo N [0000-0003-1949-0655], Hollfelder, Florian [0000-0002-1367-6312], Boroviak, Thorsten E [0000-0001-8703-8949], Apollo - University of Cambridge Repository |
| المصدر: | Development (Cambridge, England). 149(20) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Agarose microgels, Microgels, Epiblast, Sepharose, Callithrix, Cell Differentiation, Activins, Lineage specification, Primate embryogenesis, Embryonic disc, Animals, Amnion, Molecular Biology, Germ Layers, Developmental Biology |
| الوصف: | Funder: Wellcome Trust; Id: http://dx.doi.org/10.13039/100010269Test Funder: Royal Society; Id: http://dx.doi.org/10.13039/501100000288Test Funder: AstraZeneca; Id: http://dx.doi.org/10.13039/100004325Test Funder: University of Cambridge; Id: http://dx.doi.org/10.13039/501100000735Test The early specification and rapid growth of extraembryonic membranes are distinctive hallmarks of primate embryogenesis. These complex tasks are resolved through an intricate combination of signals controlling the induction of extraembryonic lineages and, at the same time, safeguarding the pluripotent epiblast. Here, we delineate the signals orchestrating primate epiblast and amnion identity. We encapsulated marmoset pluripotent stem cells into agarose microgels and identified culture conditions for the development of epiblast- and amnion-spheroids. Spatial identity mapping authenticated spheroids generated in vitro by comparison to marmoset embryos in vivo. We leveraged the microgel system to functionally interrogate the signalling environment of the postimplantation primate embryo. Single-cell profiling of the resulting spheroids demonstrated that ACTIVIN/NODAL signalling is required for embryonic lineage identity. BMP4 promoted amnion formation and maturation, which was counteracted by FGF-signalling. Our combination of microgel culture, single-cell profiling and spatial identity mapping provides a powerful approach to decipher the essential cues for embryonic and extraembryonic lineage formation in primate embryogenesis. |
| وصف الملف: | application/pdf; text/xml |
| تدمد: | 1477-9129 1000-1026 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e6ff95a3aa5fd46f935143a959e8d6bTest https://pubmed.ncbi.nlm.nih.gov/36125063Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6e6ff95a3aa5fd46f935143a959e8d6b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779129 10001026 |
|---|