Electron capture dissociation ofb2+ peptide fragments reveals the presence of the acylium ion structure
| العنوان: | Electron capture dissociation ofb2+ peptide fragments reveals the presence of the acylium ion structure |
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| المؤلفون: | Roman A. Zubarev, Bogdan A. Budnik, Kim F. Haselmann |
| المصدر: | Rapid Communications in Mass Spectrometry. 14:2242-2246 |
| بيانات النشر: | Wiley, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Electron-capture dissociation, Chemistry, Organic Chemistry, Analytical chemistry, Peptide, Protonation, Small molecule, Analytical Chemistry, Ion, Oxazolone, chemistry.chemical_compound, Crystallography, Ab initio quantum chemistry methods, Molecule, Spectroscopy |
| الوصف: | Electron capture dissociation (ECD) of peptides and their fragments has now been extended to b ( n) ( 2+) ions, where it also produced far more structural information than collisional activation. Interestingly, b ( n) ( 2+) ions revealed abundant loss of CO from radical monocations and the presence of c ((n - 1)) ( +.) fragments. The CO loss from peptide radical cations is unusual and was attributed to neutralization of the -C identical with O(+) group in the acylium ion structure, supported by the observation of c ( (n - 1)) ( +.) ions that can only be formed from an open-chain ion. These characteristic features were most prominent for b ( 12)( 2+) ions of renin substrate and least prominent for b ( n) ( 2+) ions of substance P (n = 9,10). Totally, out of seven b ( n) ( 2+) ions studied, CO loss above 3% level was present in all spectra as well as c ( (n - 1))( +.) fragments of three species, suggesting that the acylium ion structure plays a significant role for at least some b ( 2+) ions. This is an unexpected result in view of the literature data for small, singly charged b ions, for which the protonated oxazolone structure is favoured in ab initio calculations. Apparently, more studies are required before extrapolating the small molecule results to large species. The CO loss in ECD can be used for distinguishing between b and y ions in the MS/MS spectrum of larger molecules. |
| تدمد: | 1097-0231 0951-4198 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::e9761eb3c9846068f3a88c6e6fad2b55Test https://doi.org/10.1002/1097-0231Test(20001215)14:23<2242::aid-rcm158>3.0.co;2-o |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........e9761eb3c9846068f3a88c6e6fad2b55 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970231 09514198 |
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