Altered Left Ventricular Ion Channel Transcriptome in a High-Fat-Fed Rat Model of Obesity: Insight into Obesity-Induced Arrhythmogenesis
| العنوان: | Altered Left Ventricular Ion Channel Transcriptome in a High-Fat-Fed Rat Model of Obesity: Insight into Obesity-Induced Arrhythmogenesis |
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| المؤلفون: | Marianne Yon, Mark R. Boyett, Joseph Yanni Gerges, John P.H. Wilding, Lucy Pickavance, Kun Jian, Reza Ashrafi, Henggui Zhang, Gershan Davis, George Hart |
| المصدر: | Journal of obesity Journal of Obesity, Vol 2016 (2016) Journal of Obesity |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, medicine.medical_specialty, lcsh:Internal medicine, Normal diet, Article Subject, Endocrinology, Diabetes and Metabolism, Heart Ventricles, 030204 cardiovascular system & hematology, Ryanodine receptor 2, Polymerase Chain Reaction, Ventricular action potential, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Internal medicine, Gene expression, medicine, Animals, Obesity, Rats, Wistar, lcsh:RC31-1245, Voltage-dependent calcium channel, business.industry, Arrhythmias, Cardiac, A300, Dietary Fats, Rats, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Gene Expression Regulation, Ventricle, cardiovascular system, Calcium Channels, business, Erg, Research Article |
| الوصف: | Introduction. Obesity is increasingly common and is associated with an increased prevalence of cardiac arrhythmias. The aim of this study was to see whether in obesity there is proarrhythmic gene expression of ventricular ion channels and related molecules.Methods and Results. Rats were fed on a high-fat diet and compared to control rats on a normal diet (n=8). After 8 weeks, rats on the high-fat diet showed significantly greater weight gain and higher adiposity. Left ventricle samples were removed at 8 weeks and mRNA expression of ion channels and other molecules was measured using qPCR. Obese rats had significant upregulation ofCav1.2, HCN4,Kir2.1, RYR2, NCX1, SERCA2a, and RYR2 mRNA and downregulation of ERG mRNA. In the case of HCN4, it was confirmed that there was a significant increase in protein expression. The potential effects of the mRNA changes on the ventricular action potential and intracellular Ca2+transient were predicted using computer modelling. Modelling predicted prolongation of the ventricular action potential and an increase in the intracellular Ca2+transient, both of which would be expected to be arrhythmogenic.Conclusion. High-fat diet causing obesity results in arrhythmogenic cardiac gene expression of ion channels and related molecules. |
| وصف الملف: | application/pdf; text/xhtml |
| اللغة: | English |
| تدمد: | 2090-0708 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::744388b604aa37b297d2b194ab146c9cTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....744388b604aa37b297d2b194ab146c9c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20900708 |
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