Distinct Lysosomal Network Protein Profiles in Parkinsonian Syndrome Cerebrospinal Fluid
| العنوان: | Distinct Lysosomal Network Protein Profiles in Parkinsonian Syndrome Cerebrospinal Fluid |
|---|---|
| المؤلفون: | William W. Seeley, Bruce L. Miller, Andrea Boman, Julio C. Rojas, Anna Karydas, Katarina Kågedal, Adam L. Boxer, Per Svenningsson, Samuel P.S. Svensson |
| المصدر: | Journal of Parkinson's disease, vol 6, iss 2 Journal of Parkinson's Disease |
| بيانات النشر: | eScholarship, University of California, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Research Report, Male, Pathology, Aging, Parkinson's disease, parkinson’s disease, Vesicular Transport Proteins, Neurodegenerative, corticobasal degeneration (CBD), 0302 clinical medicine, Cerebrospinal fluid, 80 and over, Corticobasal degeneration, 2.1 Biological and endogenous factors, Supranuclear Palsy, Aetiology, parkinson’s disease (PD), Aged, 80 and over, screening and diagnosis, Middle Aged, 3. Good health, Cell and molecular biology, Detection, Tauopathies, Neurological, Female, Supranuclear Palsy, Progressive, Microtubule-Associated Proteins, 4.2 Evaluation of markers and technologies, Adult, medicine.medical_specialty, CSF, and over, Biology, Progressive supranuclear palsy, Parkinsonian syndromes, 03 medical and health sciences, Cellular and Molecular Neuroscience, Rare Diseases, Parkinsonian Disorders, Progressive, Clinical Research, Lysosomal-Associated Membrane Protein 2, medicine, Acquired Cognitive Impairment, Humans, Aged, Neurosciences, Lysosome-Associated Membrane Glycoproteins, progressive supranuclear palsy, medicine.disease, eye diseases, nervous system diseases, Brain Disorders, 030104 developmental biology, Orphan Drug, Clinical diagnosis, parkinson's disease, Dementia, Neurology (clinical), progressive supranuclear palsy (PSP), Biochemistry and Cell Biology, Lysosomes, Neuroscience, 030217 neurology & neurosurgery, Biomarkers, corticobasal degeneration |
| الوصف: | Background: Clinical diagnosis of parkinsonian syndromes like Parkinson’s disease (PD), corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP) is hampered by overlapping symptomatology and lack of diagnostic biomarkers, and definitive diagnosis is only possible post-mortem. Objective: Since impaired protein degradation plays an important role in many neurodegenerative disorders, we hypothesized that profiles of select lysosomal network proteins in cerebrospinal fluid could be differentially expressed in these parkinsonian syndromes. Methods: Cerebrospinal fluid samples were collected from PD patients (n = 18), clinically diagnosed 4-repeat tauopathy patients; corticobasal syndrome (CBS) (n = 3) and PSP (n = 8); and pathologically diagnosed PSP (n = 8) and CBD patients (n = 7). Each patient set was compared to its appropriate control group consisting of age and gender matched individuals. Select lysosomal network protein levels were detected via Western blotting. Factor analysis was used to test the diagnostic sensitivity, specificity and accuracy of the select lysosomal network protein expression profiles. Results: PD, CBD and PSP were markedly different in their cerebrospinal fluid lysosomal network protein profiles. Lysosomal-associated membrane proteins 1 and 2 were significantly decreased in PD; early endosomal antigen 1 was decreased and lysozyme increased in PSP; and lysosomal-associated membrane proteins 1 and 2, microtubule-associated protein 1 light chain 3 and lysozyme were increased in CBD. A panel of lysosomal-associated membrane protein 2, lysozyme and microtubule-associated protein 1 light chain discriminated between controls, PD and 4-repeat tauopathies. Conclusions: This study offers proof of concept that select lysosomal network proteins are differentially expressed in cerebrospinal fluid of Parkinson’s disease, corticobasal syndrome and progressive supranuclear palsy. Lysosomal network protein analysis could be further developed as a diagnostic fluid biomarker in parkinsonian syndromes. |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::459c26600bcb5c2124287254678a5526Test https://escholarship.org/uc/item/9287g81mTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....459c26600bcb5c2124287254678a5526 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |