دورية أكاديمية
A novel missense variant in $ATP11C$ is associated with reduced red blood cell phosphatidylserine flippase activity and mild hereditary hemolytic anemia
| العنوان: | A novel missense variant in $ATP11C$ is associated with reduced red blood cell phosphatidylserine flippase activity and mild hereditary hemolytic anemia |
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| المؤلفون: | van Dijk, Myrthe J, van Oirschot, Brigitte A, Harrison, Alexander N, Recktenwald, Steffen M, Qiao, Min, Stommen, Amaury, Cloos, Anne‐Sophie, Vanderroost, Juliette, Terrasi, Romano, Dey, Kuntal, Bos, Jennifer, Rab, Minke A E, Bogdanova, Anna, Minetti, Giampaolo, Muccioli, Giulio G, Tyteca, Donatienne, Egée, Stéphane, Kaestner, Lars, Molday, Robert S, van Beers, Eduard J, van Wijk, Richard |
| المصدر: | van Dijk, Myrthe J; van Oirschot, Brigitte A; Harrison, Alexander N; Recktenwald, Steffen M; Qiao, Min; Stommen, Amaury; Cloos, Anne‐Sophie; Vanderroost, Juliette; Terrasi, Romano; Dey, Kuntal; Bos, Jennifer; Rab, Minke A E; Bogdanova, Anna; Minetti, Giampaolo; Muccioli, Giulio G; Tyteca, Donatienne; Egée, Stéphane; Kaestner, Lars; Molday, Robert S; van Beers, Eduard J; van Wijk, Richard (2023). A novel missense variant in $ATP11C$ is associated with reduced red blood cell phosphatidylserine flippase activity and mild hereditary hemolytic anemia. American Journal of Hematology, 98(12):1877-1887. |
| بيانات النشر: | Wiley-Blackwell Publishing, Inc. |
| سنة النشر: | 2023 |
| المجموعة: | University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| مصطلحات موضوعية: | Institute of Veterinary Physiology, 570 Life sciences, biology, Hematology |
| الوصف: | Adenosine Triphosphatase (ATPase) Phospholipid Transporting 11C gene (ATP11C) encodes the major phosphatidylserine (PS) flippase in human red blood cells (RBCs). Flippases actively transport phospholipids (e.g., PS) from the outer to the inner leaflet to establish and maintain phospholipid asymmetry of the lipid bilayer of cell membranes. This asymmetry is crucial for survival since externalized PS triggers phagocytosis by splenic macrophages. Here we report on pathophysiological consequences of decreased flippase activity, prompted by a patient with hemolytic anemia and hemizygosity for a novel c.2365C > T p.(Leu789Phe) missense variant in ATP11C. ATP11C protein expression was strongly reduced by 58% in patient‐derived RBC ghosts. Furthermore, functional characterization showed only 26% PS flippase activity. These results were confirmed by recombinant mutant ATP11C protein expression in HEK293T cells, which was decreased to 27% compared to wild type, whereas PS‐stimulated ATPase activity was decreased by 57%. Patient RBCs showed a mild increase in PS surface exposure when compared to control RBCs, which further increased in the most dense RBCs after RBC storage stress. The increase in PS was not due to higher global membrane content of PS or other phospholipids. In contrast, membrane lipid lateral distribution showed increased abundance of cholesterol‐enriched domains in RBC low curvature areas. Finally, more dense RBCs and subtle changes in RBC morphology under flow hint toward alterations in flow behavior of ATP11C‐deficient RBCs. Altogether, ATP11C deficiency is the likely cause of hemolytic anemia in our patient, thereby underlining the physiological role and relevance of this flippase in human RBCs. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0361-8609 |
| العلاقة: | https://www.zora.uzh.ch/id/eprint/237166/8/van_Dijk_American_J_Hematol_2023.pdfTest; info:pmid/37671681; urn:issn:0361-8609 |
| DOI: | 10.5167/uzh-237166 |
| DOI: | 10.1002/ajh.27088 |
| الإتاحة: | https://doi.org/10.5167/uzh-23716610.1002/ajh.27088Test https://www.zora.uzh.ch/id/eprint/237166Test/ https://www.zora.uzh.ch/id/eprint/237166/8/van_Dijk_American_J_Hematol_2023.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess ; Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.18C1EC57 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 03618609 |
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| DOI: | 10.5167/uzh-237166 |