American Ginseng Regulates Gene Expression to Protect against Premature Ovarian Failure in Rats
| العنوان: | American Ginseng Regulates Gene Expression to Protect against Premature Ovarian Failure in Rats |
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| المؤلفون: | Haixue Kuang, Ji Li, Nannan Xing, Pengling Ge, Dongwei Han, Lei Zhu |
| المصدر: | BioMed Research International, Vol 2015 (2015) BioMed Research International |
| بيانات النشر: | Hindawi Limited, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | medicine.medical_specialty, Vinyl Compounds, Article Subject, endocrine system diseases, media_common.quotation_subject, Panax, lcsh:Medicine, Ovary, 12E7 Antigen, Primary Ovarian Insufficiency, Dinoprostone, General Biochemistry, Genetics and Molecular Biology, Follicle-stimulating hormone, Western blot, Antigens, CD, Internal medicine, Cyclohexenes, medicine, Animals, Humans, Ovulation, American ginseng, media_common, General Immunology and Microbiology, medicine.diagnostic_test, biology, Plant Extracts, lcsh:R, General Medicine, Luteinizing Hormone, medicine.disease, biology.organism_classification, female genital diseases and pregnancy complications, Rats, Premature ovarian failure, medicine.anatomical_structure, Endocrinology, Gene Expression Regulation, Female, Follicle Stimulating Hormone, Human, Luteinizing hormone, Cell Adhesion Molecules, Research Article, Hormone |
| الوصف: | Premature ovarian failure (POF) is defined as lost ovarian functions before the age of 40. Three possible molecular markers (PLA2G4A,miR-29a, andmiR-144) have been identified in our previous study by integrated analysis of mRNA and miRNA expression profiles. The present study aimed to evaluate American ginseng root’s protective potential against POF by studying transcriptional and protein variations between American ginseng treatments and controls in rats. 4-Vinylcyclohexene diepoxide (VCD) was administered to rats for 14 days to induce POF. Additionally, American ginseng was administered to POF rats for one month, andPLA2G4A,miR-29a, andmiR-144expressions were measured in rat ovaries by qRT-PCR. PLA2G4A protein expression was examined by Western Blot, and PGE2, LH, FSH, and E2 serum levels were detected by ELISA.PLA2G4AmRNA and protein were downregulated in American ginseng-treated rats,miR-29aandmiR-144levels increased, and PGE2serum levels decreased, while LH, FSH, and E2 increased compared to POF induction alone. Analysis of transcriptional and protein variations suggested that American ginseng protects the ovary against POF by regulating prostaglandin biosynthesis, ovulation, and preventing ovarian aging. High hormone levels (PGE2, FSH, and LH) were reduced, and E2 secretion approached normal levels, leading to improved POF symptoms and abnormal ovulation. |
| وصف الملف: | text/xhtml |
| تدمد: | 2314-6141 2314-6133 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aff4adfb6eb81bd8a4f9655655b0d263Test https://doi.org/10.1155/2015/767124Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....aff4adfb6eb81bd8a4f9655655b0d263 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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