دورية أكاديمية
Thrombin and histamine stimulate endothelial nitric-oxide synthase phosphorylation at Ser1177 via an AMPK mediated pathway independent of PI3K-Akt
| العنوان: | Thrombin and histamine stimulate endothelial nitric-oxide synthase phosphorylation at Ser1177 via an AMPK mediated pathway independent of PI3K-Akt |
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| المؤلفون: | Thors, Brynhildur, Halldorsson, Haraldur, Thorgeirsson, Gudmundur |
| بيانات النشر: | Elsevier Science B.V. |
| سنة النشر: | 2007 |
| المجموعة: | Hirsla - Landspítali University Hospital research archive |
| مصطلحات موضوعية: | 1-Phosphatidylinositol 3-Kinase, Ca(2+)-Calmodulin Dependent Protein Kinase, Thrombin, Signal Transduction, Proto-Oncogene Protein, Phosphoserine, Nitric Oxide Synthase Type III, Multienzyme Complexes |
| الوصف: | To access publisher full text version of this article. Please click on the hyperlink in Additional Links field ; Histamine and thrombin cause phosphorylation and activation of endothelial NO-synthase (eNOS) on Ser1177. We tested the role of various protein kinases in mediating this effect in human umbilical vein endothelial cells. Inhibition of the Ca2+/calmodulin-dependent protein kinase II or phosphoinositide 3-kinase (PI3K) had no effect. H89, an inhibitor of both protein kinase A (PKA) and 5'-AMP-activated protein kinase (AMPK), strongly inhibited phosphorylation and activity of eNOS. Conversely, the PKA inhibitor Rp-adenosine 3 '5'-cyclic monophosphate (cAMPS) had no effect and eNOS was not phosphorylated by treatments that affect cAMP levels. Thrombin and histamine caused phosphorylation of AMPK on Thr172 as well as on its downstream target acetyl-CoA carboxylase. Activation of AMPK using AICAR or CCCP also resulted in eNOS phosphorylation. We conclude that histamine and thrombin cause eNOS phosphorylation in an AMPK mediated manner, independent of P13K-Akt. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | YES |
| اللغة: | English |
| تدمد: | 0014-5793 |
| العلاقة: | http://www.sciencedirect.com/science/article/B6T36-4D2C7CS-D/2/0602dcd9f2daacfc90a7c0dbf93de7b2Test; FEBS Lett. 2004, 573(1-3):175-80; http://hdl.handle.net/2336/13600Test; FEBS letters |
| DOI: | 10.1016/j.febslet.2004.07.078 |
| الإتاحة: | https://doi.org/10.1016/j.febslet.2004.07.078Test http://hdl.handle.net/2336/13600Test |
| رقم الانضمام: | edsbas.5A365CE5 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00145793 |
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| DOI: | 10.1016/j.febslet.2004.07.078 |