Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers
| العنوان: | Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers |
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| المؤلفون: | Manhee Lee, Gui Sek Bae, Urantulkhuur Battumur, Chang-Hyun Kim |
| المصدر: | Asian-Australasian Journal of Animal Sciences |
| بيانات النشر: | Asian Australasian Association of Animal Production Societies, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Rumen, Coccus, Trimethylamine, Methanogen, Article, Ruminant Nutrition and Forage Utilization, chemistry.chemical_compound, medicine, Food science, 16S rRNA, Strain (chemistry), biology, Chloramphenicol, 0402 animal and dairy science, Kanamycin, 04 agricultural and veterinary sciences, mcrA, biology.organism_classification, 16S ribosomal RNA, 040201 dairy & animal science, Methanoculleus bourgensis, chemistry, Animal Science and Zoology, Food Science, medicine.drug |
| الوصف: | Objective To isolate and identify new methanogens from the rumen of Holstein steers in Korea. Methods Representative rumen contents were obtained from three ruminally cannulated Holstein steers (793±8 kg). Pre-reduced media were used for the growth and isolation of methanogens. Optimum growth temperature, pH, and sodium chloride (NaCl) concentration as well as substrate utilization and antibiotic tolerance were investigated to determine the physiological characteristics of the isolated strain. Furthermore, the isolate was microscopically studied for its morphology. Polymerase chain reaction of 16S rRNA and mcrA gene-based amplicons was used for identification. Results One strain designated as KOR-2 was isolated and found to be a non-motile irregular coccus with a diameter of 0.2 to 0.5 μm. KOR-2 utilized H2+CO2 and formate but was unable to metabolize acetate, methanol, trimethylamine, 2-propanol, and isobutanol for growth and methane production. The optimum temperature and pH for the growth of KOR-2 were 38°C and 6.8 to 7.0, respectively, while the optimum NaCl concentration essential for KOR-2 growth was 1.0% (w/v). KOR-2 tolerated ampicillin, penicillin G, kanamycin, spectromycin, and tetracycline. In contrast, the cell growth was inhibited by chloramphenicol. Phylogenetic analysis of 16S rRNA and mcrA genes revealed the relatedness between KOR-2 and Methanoculleus bourgensis. Conclusion Based on the physiological and phylogenetic characteristics, KOR-2 was thought to be a new strain within the genus Methanoculleus and named Methanoculleus bourgensis KOR-2. |
| تدمد: | 1976-5517 1011-2367 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a79e97aabd3e8d2dd4c411fec818cbfTest https://doi.org/10.5713/ajas.18.0409Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5a79e97aabd3e8d2dd4c411fec818cbf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19765517 10112367 |
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