Control ofListeriaSuperoxide Dismutase by Phosphorylation
| العنوان: | Control ofListeriaSuperoxide Dismutase by Phosphorylation |
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| المؤلفون: | Olivier Dussurget, Marie-Anne Nahori, Cristel Archambaud, Javier Pizarro-Cerdá, Pascale Cossart |
| المساهمون: | Interactions Bactéries-Cellules (UIBC), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), ProdInra, Migration |
| المصدر: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2006, 281 (42), pp.31812-31822. ⟨10.1074/jbc.M606249200⟩ Journal of Biological Chemistry, 2006, 281 (42), pp.31812-31822. ⟨10.1074/jbc.M606249200⟩ Journal of Biological Chemistry 42 (281), 31812-31822. (2006) |
| بيانات النشر: | Elsevier BV, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Threonine, Virulence, Biology, REACTIVE OXIGEN SPECIES, medicine.disease_cause, Biochemistry, Gene Expression Regulation, Enzymologic, Virulence factor, Serine, Superoxide dismutase, Mice, 03 medical and health sciences, chemistry.chemical_compound, Listeria monocytogenes, medicine, Animals, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Phosphorylation, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Molecular Biology, 030304 developmental biology, chemistry.chemical_classification, Mice, Inbred BALB C, 0303 health sciences, Reactive oxygen species, Superoxide Dismutase, 030306 microbiology, Superoxide, Macrophages, GENE EXPRESSION REGULATION, Gene Expression Regulation, Bacterial, Cell Biology, Cell biology, chemistry, biology.protein, LISTERIA MONOCYTOGENES, SUPEROXIDE DISMUTASE, SECRETION, VIRULENCE, Female, Reactive Oxygen Species, Protein Processing, Post-Translational |
| الوصف: | International audience; Superoxide dismutases (SODs) are enzymes that protect organisms against superoxides and reactive oxygen species (ROS) produced during their active metabolism. ROS are major mediators of phagocytes microbicidal activity. Here we show that the cytoplasmic Listeria monocytogenes MnSOD is phosphorylated on serine and threonine residues and less active when bacteria reach the stationary phase. We also provide evidence that the most active nonphosphorylated form of MnSOD can be secreted via the SecA2 pathway in culture supernatants and in infected cells, where it becomes phosphorylated. A Deltasod deletion mutant is impaired in survival within macrophages and is dramatically attenuated in mice. Together, our results demonstrate that the capacity to counteract ROS is an essential component of L. monocytogenes virulence. This is the first example of a bacterial SOD post-translationally controlled by phosphorylation, suggesting a possible new host innate mechanism to counteract a virulence factor. |
| وصف الملف: | application/pdf |
| تدمد: | 1083-351X 0021-9258 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd8950358ef5ebe054fee695360c00a8Test https://doi.org/10.1074/jbc.m606249200Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cd8950358ef5ebe054fee695360c00a8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1083351X 00219258 |
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