A cross-species approach for the identification of Drosophila male sterility genes
| العنوان: | A cross-species approach for the identification of Drosophila male sterility genes |
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| المؤلفون: | Masatoshi Tomaru, Toshiyuki Takano-Shimizu-Kouno, Mihoko Nakatsuka, Kimihide Ibaraki, Yu Miyazaki, Masahide Watanabe, Timothy L. Karr, Machi Shirakami, Rikako Sanuki, Takashi Ohsako |
| المصدر: | G3: Genes, Genomes, Genetics, Vol 11, Iss 8 (2021) G3: Genes|Genomes|Genetics |
| بيانات النشر: | Oxford University Press, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | AcademicSubjects/SCI01140, Male, Sterility, AcademicSubjects/SCI00010, QH426-470, AcademicSubjects/SCI01180, male sterility, Germline, Male infertility, 03 medical and health sciences, 0302 clinical medicine, Testis, medicine, Genetics, Animals, Drosophila Proteins, Spermatogenesis, Molecular Biology, Drosophila, human male infertility, Genetics (clinical), Infertility, Male, 030304 developmental biology, 0303 health sciences, biology, fungi, Mutant Screen Report, biology.organism_classification, medicine.disease, Phenotype, Sperm, Rack1, Sexual reproduction, Drosophila melanogaster, AcademicSubjects/SCI00960, cross-species approach, 030217 neurology & neurosurgery |
| الوصف: | Male reproduction encompasses many essential cellular processes and interactions. As a focal point for these events, sperm offer opportunities for advancing our understanding of sexual reproduction at multiple levels during development. Using male sterility genes identified in human, mouse, and fruit fly databases as a starting point, 103 Drosophila melanogaster genes were screened for their association with male sterility by tissue-specific RNAi knockdown and CRISPR/Cas9-mediated mutagenesis. This list included 56 genes associated with male infertility in the human databases, but not found in the Drosophila database, resulting in the discovery of 63 new genes associated with male fertility in Drosophila. The phenotypes identified were categorized into six distinct classes affecting sperm development. Interestingly, the second largest class (Class VI) caused sterility despite apparently normal testis and sperm morphology suggesting that these proteins may have functions in the mature sperm following spermatogenesis. We focused on one such gene, Rack 1, and found that it plays an important role in two developmental periods, in early germline cells or germline stem cells and in spermatogenic cells or sperm. Taken together, many genes are yet to be identified and their role in male reproduction, especially after ejaculation, remains to be elucidated in Drosophila, where a wealth of data from human and other model organisms would be useful. |
| اللغة: | English |
| تدمد: | 2160-1836 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90785f9243d0eba9595b332995699858Test https://academic.oup.com/g3journal/article-lookup/doi/10.1093/g3journal/jkab183Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....90785f9243d0eba9595b332995699858 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21601836 |
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