Requirement of the proteasome for the trimming of signal peptide-derived epitopes presented by the nonclassical major histocompatibility complex class I molecule HLA-E
| العنوان: | Requirement of the proteasome for the trimming of signal peptide-derived epitopes presented by the nonclassical major histocompatibility complex class I molecule HLA-E |
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| المؤلفون: | Felicity A. Bland, Andrew J. McMichael, Veronique M. Braud, Marius K. Lemberg, Bruno Martoglio |
| المساهمون: | Medical Research Council Human Immunology Unit, University of Oxford [Oxford]- John Radcliffe Hospital [Oxford University Hospital], Institute of Biochemistry, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Medical Research Council, CNRS, Swiss National Science Foundation, National Competence Centre for Research on Neuronal Plasticity and Repair, Boehringer Ingelheim Fellowship |
| المصدر: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2003, 278 (36), pp.33747-52. ⟨10.1074/jbc.M305593200⟩ |
| بيانات النشر: | HAL CCSD, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Transcription, Genetic, Peptide, MESH: Flow Cytometry, MESH: Amino Acid Sequence, Ligands, MESH: Multienzyme Complexes, Biochemistry, Epitope, MESH: Histocompatibility Antigens Class I, MESH: Dose-Response Relationship, Drug, Epitopes, MESH: Protein Structure, Tertiary, 0302 clinical medicine, Cytosol, HLA-E, MESH: Cytosol, HLA Antigens, MESH: Ligands, Aspartic Acid Endopeptidases, MESH: Serine Endopeptidases, Peptide sequence, MESH: HLA Antigens, chemistry.chemical_classification, 0303 health sciences, Signal peptidase, MESH: Peptides, MESH: Proteasome Endopeptidase Complex, MESH: Aspartic Endopeptidases, Serine Endopeptidases, Flow Cytometry, Cysteine Endopeptidases, MESH: Protein Biosynthesis, MESH: Oligopeptides, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Membrane Proteins, Signal peptide peptidase, Oligopeptides, Plasmids, Signal peptide, Proteasome Endopeptidase Complex, MESH: Epitopes, MESH: Peptide Hydrolases, Molecular Sequence Data, Biology, Cysteine Proteinase Inhibitors, Major histocompatibility complex, Transfection, MESH: Acids, Cell Line, MESH: Cysteine Proteinase Inhibitors, 03 medical and health sciences, MESH: Acetylcysteine, Multienzyme Complexes, MESH: Plasmids, Humans, Amino Acid Sequence, Molecular Biology, Alleles, 030304 developmental biology, HLA-G Antigens, MESH: Humans, MESH: Molecular Sequence Data, Dose-Response Relationship, Drug, MESH: Alleles, MESH: Transcription, Genetic, MESH: Transfection, Histocompatibility Antigens Class I, Membrane Proteins, Cell Biology, Acetylcysteine, Protein Structure, Tertiary, MESH: Cell Line, chemistry, Protein Biosynthesis, biology.protein, Peptides, Acids, 030215 immunology, Peptide Hydrolases, MESH: Cysteine Endopeptidases |
| الوصف: | The nonclassical major histocompatibility complex class I molecule HLA-E acts as a ligand for CD94/NKG2 receptors on the surface of natural killer cells and a subset of T cells. HLA-E presents closely related nonameric peptide epitopes derived from the highly conserved signal sequences of classical major histocompatibility complex class I molecules as well as HLA-G. Their generation requires cleavage of the signal sequence by signal peptidase followed by the intramembrane-cleaving aspartic protease, signal peptide peptidase. In this study, we have assessed the subsequent proteolytic requirements leading to generation of the nonameric HLA-E peptide epitopes. We show that proteasome activity is required for further processing of the peptide generated by signal peptide peptidase. This constitutes the first example of capture of a naturally derived short peptide by the proteasome, producing a class I peptide ligand. |
| اللغة: | English |
| تدمد: | 0021-9258 1083-351X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5cf79e06d6e2fc7e8888d53164d76277Test https://hal.archives-ouvertes.fr/hal-00170998Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5cf79e06d6e2fc7e8888d53164d76277 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219258 1083351X |
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