Phylogenetic analyses of rhizobia isolated from nodules of lupinus angustifolius in northern tunisia reveal devosia sp. As a new microsymbiont of lupin species
| العنوان: | Phylogenetic analyses of rhizobia isolated from nodules of lupinus angustifolius in northern tunisia reveal devosia sp. As a new microsymbiont of lupin species |
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| المؤلفون: | José Manuel Palacios, José Javier Pueyo, Mohamed Mars, Abdelhakim Msaddak, Luis Rey, Juan Imperial |
| المساهمون: | Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie), Agencia Estatal de Investigación (España), CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI), Rey, Luis (0000-0003-3477-6942), Imperial, Juan (0000-0002-5002-6458), Palacios, José Manuel (0000-0002-2541-8812), Mars, Mohamed (0000-0001-9427-6419), Pueyo, José J. (0000-0003-0337-4078) |
| المصدر: | Agronomy, Vol 11, Iss 1510, p 1510 (2021) Agronomy Volume 11 Issue 8 |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Devosia, food.ingredient, Root nodule, Tunisia, microsymbiont, Microvirga, Lupinus angustifolius, Bradyrhizobium, Rhizobia, 03 medical and health sciences, food, Genus, Botany, Microsym-biont, 030304 developmental biology, 0303 health sciences, biology, Phylogenetic tree, 030306 microbiology, Microbiota, Agriculture, Biodiversity, biology.organism_classification, Lupin, Agronomy and Crop Science, Rhizobium |
| الوصف: | 17 Pág. Centro de Biotecnología y Genómica de Plantas Thirty-two bacterial isolates were obtained from root nodules of Lupinus angustifolius growing in Northern Tunisia. Phylogenetic analyses based on recA and gyrB partial gene sequences grouped the strains into six clusters: four clusters belonged to the genus Bradyrhizobium (22 isolates), one to Microvirga (8 isolates) and one to Devosia (2 isolates), a genus that has not been previously reported to nodulate lupin. Representative strains of each group were further characterized. MultiLocus Sequence Analysis (MLSA) based on recA and glnII gene sequences separated the strains within the genus Bradyrhizobium into four divergent clusters related to B. canariense, B. liaoningense, B. lupini, and B. algeriense, respectively. The latter might constitute a new Bradyrhizobium species. The strains in the Microvirga cluster showed high identity with M. tunisiensis. The Devosia isolates might also represent a new species within this genus. An additional phylogenetic analysis based on the symbiotic gene nodC affiliated the strains to symbiovars genistearum, mediterranense, and to a possibly new symbiovar. These results altogether contributed to the existing knowledge on the genetic diversity of lupin-nodulating microsymbionts and revealed a likely new, fast-growing, salt-tolerant rhizobial species within the genus Devosia as a potentially useful inoculant in agricultural practices or landscape restoration. This research was funded by the Ministry of High Education and Research Development, Tunisia, and by the Agencia Estatal de Investigación, AEI, Spain, grant numbers AGL2017-88381-R and RTI2018-094985-B-100. The APC was funded in part by CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4f0ff70c64a1f96f3f94c321102481bTest http://hdl.handle.net/10261/286681Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f4f0ff70c64a1f96f3f94c321102481b |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |