المؤلفون: Jérôme Santolini, Jeremy Astier, David Wendehenne, Sylvain Jeandroz, Arnaud Mounier

المساهمون: Agroécologie [Dijon], Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC), ANR-18-CE20-0022,ALGAE-NOS,Evolution et Fonction des NO-Synthases de plantes(2018), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), EL Mjiyad, Noureddine, Université Paris Saclay (COmUE), ProdInra, Migration

المصدر: Journal of Experimental Botany
Journal of Experimental Botany, Oxford University Press (OUP), 2019, ⟨10.1093/jxb/erz088⟩
Journal of Experimental Botany, 2019, ⟨10.1093/jxb/erz088⟩
14. POG International Conference on Reactive Oxygen and Nitrogen Species in Plants
14. POG International Conference on Reactive Oxygen and Nitrogen Species in Plants, Jul 2019, Munich, Germany

مصطلحات موضوعية: [SDE] Environmental Sciences, 0106 biological sciences, Algae, Physiology, Lineage (evolution), [SDV]Life Sciences [q-bio], Regulator, Plant Science, Signalling, 01 natural sciences, Nitric oxide, Evolution, Molecular, 03 medical and health sciences, chemistry.chemical_compound, cyclic nucleotide-gated channel, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Animals, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Phosphodiesterase, Cyclic GMP, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, CGMP, biology, Mechanism (biology), Kinase, Nitric oxide synthase, Plant, Plants, Guanylate cyclase, Cell biology, [SDV] Life Sciences [q-bio], chemistry, cGMP-dependent protein kinase, [SDE]Environmental Sciences, biology.protein, 010606 plant biology & botany, Signal Transduction

الوصف: Nitric oxide (NO) is a ubiquitous signalling molecule with widespread distribution in prokaryotes and eukaryotes where it is involved in countless physiological processes. While the mechanisms governing nitric oxide (NO) synthesis and signalling are well established in animals, the situation is less clear in the green lineage. Recent investigations have shown that NO synthase, the major enzymatic source for NO in animals, is absent in land plants but present in a limited number of algae. The first detailed analysis highlighted that these new NO synthases are functional but display specific structural features and probably original catalytic activities. Completing this picture, analyses were undertaken in order to investigate whether major components of the prototypic NO/cyclic GMP signalling cascades mediating many physiological effects of NO in animals were also present in plants. Only a few homologues of soluble guanylate cyclases, cGMP-dependent protein kinases, cyclic nucleotide-gated channels, and cGMP-regulated phosphodiesterases were identified in some algal species and their presence did not correlate with that of NO synthases. In contrast, S-nitrosoglutathione reductase, a critical regulator of S-nitrosothiols, was recurrently found. Overall, these findings highlight that plants do not mediate NO signalling through the classical NO/cGMP signalling module and support the concept that S-nitrosation is a ubiquitous NO-dependent signalling mechanism.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b72fd20ecc718b4795b38fe4aa974e42Test
https://hal-agrosup-dijon.archives-ouvertes.fr/hal-02065043Test

المؤلفون: Jani Kelloniemi, Alain Pugin, David Wendehenne, Benoît Poinssot, Angela Garcia-Brugger, Annick Chiltz, Hamid Manzoor

المساهمون: Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Bahauddin Zakariya University (BZU), Agrifood Research Finland, Higher Education Commission (HEC), Pakistan, Conseil Regional de Bourgogne, Caphe ANR program

المصدر: Plant Journal
Plant Journal, Wiley, 2013, 76 (3), pp.466-480. ⟨10.1111/tpj.12311⟩

مصطلحات موضوعية: 0106 biological sciences, Arabidopsis thaliana, [SDV]Life Sciences [q-bio], Arabidopsis, Oligosaccharides, Plant Science, 01 natural sciences, CALCIUM SIGNATURES, chemistry.chemical_compound, Gene Expression Regulation, Plant, SYSTEMIC ACQUIRED-RESISTANCE, Plant defense against herbivory, Plant Immunity, GENE-EXPRESSION, Calcium signaling, 0303 health sciences, IMMUNE-RESPONSES, TOBACCO CELLS, food and beverages, CYTOSOLIC CALCIUM, Elicitor, Oomycetes, Receptors, Glutamate, Biochemistry, Host-Pathogen Interactions, [SDE]Environmental Sciences, oligogalacturonides, Signal transduction, Signal Transduction, glutamate receptor, Hyaloperonospora arabidopsidis, Biology, Nitric Oxide, calcium signaling, 03 medical and health sciences, plant defense, Genetics, DNQX, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, BOTRYTIS-CINEREA, 030304 developmental biology, NITRIC-OXIDE, Arabidopsis Proteins, Cell Biology, biology.organism_classification, SALICYLIC-ACID, chemistry, PLASMA-MEMBRANE, Reactive Oxygen Species, 010606 plant biology & botany

الوصف: Like their animal counterparts, plant glutamate receptor-like (GLR) homologs are intimately associated with Ca(2+) influx through plasma membrane and participate in various physiological processes. In pathogen-associated molecular patterns (PAMP)-/elicitor-mediated resistance, Ca(2+) fluxes are necessary for activating downstream signaling events related to plant defense. In this study, oligogalacturonides (OGs), which are endogenous elicitors derived from cell wall degradation, were used to investigate the role of Arabidopsis GLRs in defense signaling. Pharmacological investigations indicated that GLRs are partly involved in free cytosolic [Ca(2+)] ([Ca(2+)]cyt) variations, nitric oxide (NO) production, reactive oxygen species (ROS) production and expression of defense-related genes by OGs. In addition, wild-type Col-0 plants treated with the glutamate-receptor antagonist 6,7-dinitriquinoxaline-2,3-dione (DNQX) had a compromised resistance to Botrytis cinerea and Hyaloperonospora arabidopsidis. Moreover, we provide genetic evidence that AtGLR3.3 is a key component of resistance against H. arabidopsidis. In addition, some OGs-triggered immune events such as defense gene expression, NO and ROS production are also to different extents dependent on AtGLR3.3. Taken together, these data provide evidence for the involvement of GLRs in elicitor/pathogen-mediated plant defense signaling pathways in Arabidopsis thaliana.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d02e3a1bd3351b1918b83710dc70555Test
https://doi.org/10.1111/tpj.12311Test

المؤلفون: Sumaira Rasul, Jeremy Astier, Wei Ma, David Wendehenne, Pauline Trapet, Sylvain Jeandroz, Angélique Besson-Bard, Gerald A. Berkowitz, Olivier Lamotte, Valérie Nicolas-Francès, Stéphane Bourque

المساهمون: Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Michigan State University [East Lansing], Michigan State University System, University of Connecticut (UCONN), the Agence Nationale de la Recherche (grant no. BLAN07-2_184783 PIANO), La Region de Bourgogne (PARI AGRALE 8 project to O.L.), and the U.S. National Science Foundation (grant no. 1146827)., Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté ( UBFC ), Laboratoire Management et Relations internationales, ESG, Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Plante - microbe - environnement : biochimie, biologie cellulaire et écologie ( PMEBBCE ), Etablissement National d'Enseignement Supérieur Agronomique de Dijon ( ENESAD ) -Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Laboratoire de Mecanique des Fluides et d'Acoustique ( LMFA ), École Centrale de Lyon ( ECL ), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS )

المصدر: Plant Physiology
Plant Physiology, American Society of Plant Biologists, 2013, 163 (2), pp.459--470. ⟨10.1104/pp.113.220624⟩
Plant Physiology, American Society of Plant Biologists, 2013, 163 (2), pp.459--470. 〈10.1104/pp.113.220624〉

مصطلحات موضوعية: 0106 biological sciences, calmodulin, Cell signaling, Calmodulin, Physiology, [SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy, Nanotechnology, Plant Science, Biology, 01 natural sciences, Nitric oxide, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, [ SDV.SA.AGRO ] Life Sciences [q-bio]/Agricultural sciences/Agronomy, plant defense, Genetics, Plant defense against herbivory, Ion channel, 030304 developmental biology, 0303 health sciences, cell signalling, Biotic stress, Cell biology, chemistry, protein S-nitrosylation, gene expression, biology.protein, plant immunity, Signal transduction, 010606 plant biology & botany

الوصف: Calcium and nitric oxide (NO) are two important biological messengers. Increasing evidence indicates that Ca2+ and NO work together in mediating responses to pathogenic microorganisms and microbe-associated molecular patterns. Ca2+ fluxes were recognized to account for NO production, whereas evidence gathered from a number of studies highlights that NO is one of the key messengers mediating Ca2+ signaling. Here, we present a concise description of the current understanding of the molecular mechanisms underlying the cross talk between Ca2+ and NO in plant cells exposed to biotic stress. Particular attention will be given to the involvement of cyclic nucleotide-gated ion channels and Ca2+ sensors. Notably, we provide new evidence that calmodulin might be regulated at the posttranslational level by NO through S-nitrosylation. Furthermore, we report original transcriptomic data showing that NO produced in response to oligogalacturonide regulates the expression of genes related to Ca2+ signaling. Deeper insight into the molecules involved in the interplay between Ca2+ and NO not only permits a better characterization of the Ca2+ signaling system but also allows us to further understand how plants respond to pathogen attack.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0440c362839fc8a7d4e22d72ba4c978aTest
https://doi.org/10.1104/pp.113.220624Test

المؤلفون: Emmanuel Koen, Valérie Nicolas-Francès, David Wendehenne, Angélique Besson-Bard, Sylvain Jeandroz, Olivier Lamotte, Stéphane Bourque

المصدر: médecine/sciences. 29:309-316

مصطلحات موضوعية: 0106 biological sciences, 0303 health sciences, 03 medical and health sciences, Philosophy, General Medicine, 01 natural sciences, Molecular biology, General Biochemistry, Genetics and Molecular Biology, 030304 developmental biology, 010606 plant biology & botany

الوصف: Le monoxyde d’azote (NO) est un mediateur physiologique associe a divers processus chez les animaux, dont l’immunite. Des travaux conduits recemment montrent que les plantes, confrontees a l’attaque d’agents pathogenes, produisent egalement du NO. Le NO est donc un acteur des voies de signalisation cellulaire activees en reponse a la reconnaissance par les plantes d’agresseurs exterieurs. L’etude des molecules cibles du NO et, plus particulierement, la caracterisation de proteines S-nitrosylees, a permis d’avoir un premier apercu des mecanismes fins inherents a ses fonctions. Le NO serait ainsi implique dans l’activation ainsi que dans la desensibilisation des voies de signalisation mobilisees lors de l’immunite chez les plantes.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::7fdceddf53feca1349e2712dd0556611Test
https://doi.org/10.1051/medsci/2013293018Test

المؤلفون: Stéphane Bourque, David Wendehenne, Jean Borges Bertoldo, Hernán Terenzi, Jeremy Astier, Angélique Besson-Bard, Olivier Lamotte

المساهمون: Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidade Federal de Santa Catarina = Federal University of Santa Catarina [Florianópolis] (UFSC), Agence Nationale de la Recherche [BLAN07-2_184783 PIANO], Burgundy State [PARI AGRALE 8], FAPESC (Fundacao de Apoio a Pesquisa Cientifica e Tecnologica do Estado de Santa Catarina), CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), MCT/FINEP (Ministerio da Ciencia e Tecnologia/Financiadora de Estudos e Projetos), CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior), Ministere Francais de l'Education Nationale, de la Recherche et de la Technologie, Burgundy Sate [FABER project] [PARI AGRALE 8]

المصدر: Biochemical Journal
Biochemical Journal, Portland Press, 2012, 447, pp.249-260. ⟨10.1042/BJ20120257⟩

مصطلحات موضوعية: Models, Molecular, 0106 biological sciences, Protein Conformation, [SDV]Life Sciences [q-bio], Nicotiana tabacum, ATPase, Molecular Sequence Data, Cell Cycle Proteins, Nitric Oxide, 01 natural sciences, Biochemistry, cryptogein, Fungal Proteins, 03 medical and health sciences, Valosin Containing Protein, Tobacco, Amino Acid Sequence, nitric oxide (no), Molecular Biology, Plant Proteins, 030304 developmental biology, Adenosine Triphosphatases, 0303 health sciences, biology, Walker motifs, Cell Biology, S-Nitrosylation, cell division cycle 48 (cdc48), Biotic stress, biology.organism_classification, AAA proteins, Protein Structure, Tertiary, Elicitor, Chaperone (protein), [SDE]Environmental Sciences, biology.protein, s-nitrosylation, plant defence responses, 010606 plant biology & botany

الوصف: NO has important physiological functions in plants, including the adaptative response to pathogen attack. We previously demonstrated that cryptogein, an elicitor of defence reaction produced by the oomycete Phytophthora cryptogea , triggers NO synthesis in tobacco. To decipher the role of NO in tobacco cells elicited by cryptogein, in the present study we performed a proteomic approach in order to identify proteins undergoing S-nitrosylation. We provided evidence that cryptogein induced the S-nitrosylation of several proteins and identified 11 candidates, including CDC48 (cell division cycle 48), a member of the AAA+ ATPase (ATPase associated with various cellular activities) family. In vitro , NtCDC48 ( Nicotiana tabacum CDC48) was shown to be poly-S-nitrosylated by NO donors and we could identify Cys 110 , Cys 526 and Cys 664 as a targets for S-nitrosylation. Cys 526 is located in the Walker A motif of the D2 domain, that is involved in ATP binding and was previously reported to be regulated by oxidative modification in Drosophila . We investigated the consequence of NtCDC48 S-nitrosylation and found that NO abolished NtCDC48 ATPase activity and induced slight conformation changes in the vicinity of Cys 526 . Similarly, substitution of Cys 526 by an alanine residue had an impact on NtCDC48 activity. More generally, the present study identified CDC48 as a new candidate for S-nitrosylation in plants facing biotic stress and further supports the importance of Cys 526 in the regulation of CDC48 by oxidative/nitrosative agents.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::121e0c63d20642b9f295e2c1fc4c5d27Test
https://doi.org/10.1042/bj20120257Test

المؤلفون: David Wendehenne, Benoît Poinssot, G. Alcaraz, Olivier Lamotte, Sumaira Rasul, C. Dubreuil-Maurizi, Emmanuel Koen, Sylvain Jeandroz

المصدر: Plant, Cell & Environment. 35:1483-1499

مصطلحات موضوعية: 0106 biological sciences, chemistry.chemical_classification, 0303 health sciences, Reactive oxygen species, biology, Physiology, fungi, Mutant, food and beverages, Plant physiology, Plant Science, biology.organism_classification, Nitrate reductase, 01 natural sciences, Nitric oxide, 03 medical and health sciences, chemistry.chemical_compound, chemistry, Biochemistry, biology.protein, Arabidopsis thaliana, 030304 developmental biology, 010606 plant biology & botany, Peroxidase, Botrytis cinerea

الوصف: Nitric oxide (NO) regulates a wide range of plant processes from development to environmental adaptation. In this study, we investigated the production and/or function of NO in Arabidopsis thaliana leaf discs and plants elicited by oligogalacturonides (OGs) and challenged with Botrytis cinerea. We provided evidence that OGs triggered a fast and long lasting NO production which was Ca(2+) dependent and involved nitrate reductase (NR). Accordingly, OGs triggered an increase of both NR activity and transcript accumulation. NO production was also sensitive to the mammalian NO synthase inhibitor L-NAME. Intriguingly, we showed that L-NAME affected NO production by interfering with NR activity, thus questioning the mechanisms of how this compound impairs NO synthesis in plants. We further demonstrated that NO modulates RBOHD-mediated reactive oxygen species (ROS) production and participates in the regulation of OG-responsive genes such as anionic peroxidase (PER4) and a β-1,3-glucanase. Mutant plants impaired in PER4 and β-1,3-glucanase, as well as Col-0 plants treated with the NO scavenger cPTIO, were more susceptible to B. cinerea. Taken together, our investigation deciphers part of the mechanisms linking NO production, NO-induced effects and basal resistance to B. cinerea.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::1ead8699c93dc5648fb733641dea97daTest
https://doi.org/10.1111/j.1365-3040.2012.02505.xTest

المؤلفون: Jeremy Astier, Sylvain La Camera, Jean-Pierre Métraux, Eliane Abou-Mansour, Christiane Gatz, Corinna Thurow, Olivier Lamotte, Gonzague Page, Mark Zander, David Wendehenne, Floriane L’Haridon

المصدر: The Plant Journal. 68:507-519

مصطلحات موضوعية: 0106 biological sciences, Regulation of gene expression, 0303 health sciences, biology, Jasmonic acid, fungi, food and beverages, Virulence, Cell Biology, Plant Science, Plant disease resistance, biology.organism_classification, 01 natural sciences, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, chemistry, Genetics, Arabidopsis thaliana, Plant hormone, Pathogen, 030304 developmental biology, 010606 plant biology & botany, Botrytis cinerea

الوصف: Summary Botrytis cinerea is a major pre- and post-harvest necrotrophic pathogen with a broad host range that causes substantial crop losses. The plant hormone jasmonic acid (JA) is involved in the basal resistance against this fungus. Despite basal resistance, virulent strains of B. cinerea can cause disease on Arabidopsis thaliana and virulent pathogens can interfere with the metabolism of the host in a way to facilitate infection of the plant. However, plant genes that are required by the pathogen for infection remain poorly described. To find such genes, we have compared the changes in gene expression induced in A. thaliana by JA with those induced after B. cinerea using genome-wide microarrays. We have identified genes that are repressed by JA but that are induced by B. cinerea. In this study, we describe one candidate gene, ATGRXS13, that encodes for a putative glutaredoxin and that exhibits such a crossed expression. In plants that are infected by this necrotrophic fungus, ATGRXS13 expression was negatively controlled by JA and TGA transcription factors but also through a JA-salicylic acid (SA) cross-talk mechanism as B. cinerea induced SA production that positively controlled ATGRXS13 expression. Furthermore, plants impaired in ATGRXS13 exhibited resistance to B. cinerea. Finally, we present a model whereby B. cinerea takes advantage of defence signalling pathways of the plant to help the colonization of its host.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::dfc548a759e31ab8a3e48478cb762cf4Test
https://doi.org/10.1111/j.1365-313x.2011.04706.xTest

المؤلفون: Jani Kelloniemi, David Wendehenne, Benoît Poinssot, Marie-Claire Héloir, Marielle Adrian, Magdalena Gamm

المساهمون: Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)

المصدر: Molecular Genetics and Genomics
Molecular Genetics and Genomics, Springer Verlag, 2011, 285 (4), pp.273-285. ⟨10.1007/s00438-011-0607-2⟩

مصطلحات موضوعية: 0106 biological sciences, Pterostilbene, [SDV]Life Sciences [q-bio], Statistics as Topic, reference gene, Phenylalanine ammonia-lyase, resveratrol, Genes, Plant, o-methyltransferase, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Reference genes, Stilbenes, Gene expression, Genetics, Vitis, Molecular Biology, Gene, DNA Primers, 030304 developmental biology, Botrytis cinerea, 2. Zero hunger, Regulation of gene expression, 0303 health sciences, biology, Reverse Transcriptase Polymerase Chain Reaction, Reproducibility of Results, General Medicine, Reference Standards, biology.organism_classification, grapevine, 3. Good health, Biochemistry, chemistry, Plasmopara viticola, plasmopara viticola, Software, 010606 plant biology & botany

الوصف: International audience; The recent publication of the grapevine genome sequence facilitates the use of qRT-PCR to study gene expression changes. For this approach, reference genes are commonly used to normalize data and their stability of expression should be systematically validated. Among grapevine defenses is the production of the antimicrobial stilbenic phytoalexins, notably the highly fungitoxic pterostilbene, which plays a crucial role in grapevine interaction with Plasmopara viticola and Botrytis cinerea. As a resveratrol O-methyltransferase (ROMT) gene involved in pterostilbene synthesis was recently identified, we investigated the accumulation of the corresponding transcripts to those of two other stilbene biosynthesis related genes phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) in response to pathogen infection. Using three computer-based statistical methods and C-t values or LRE method generated values as input data, we have first identified two reference genes (VATP16 and 60SRP) suitable for normalization of qPCR expression data obtained in grapevine leaves and berries infected by P. viticola and B. cinerea, respectively. Next, we have highlighted that the expression of ROMT is induced in P. viticola-infected leaves and also in B. cinerea-infected berries, confirming the involvement of pterostilbene in grapevine defenses.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9af9846a3532bb8adb9d33596731a74fTest
https://doi.org/10.1007/s00438-011-0607-2Test

المؤلفون: David Wendehenne, Sophie Trouvelot, Carole Dubreuil-Maurizi, Patrick Frettinger, Alain Pugin, Benoît Poinssot

المساهمون: Plante - microbe - environnement : biochimie, biologie cellulaire et écologie (PMEBBCE), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)

المصدر: Molecular Plant-Microbe Interactions
Molecular Plant-Microbe Interactions, American Phytopathological Society, 2010, 23 (8), pp.1012-1021. ⟨10.1094/MPMI-23-8-1012⟩

مصطلحات موضوعية: NADPH OXIDASE-DEPENDENT, Phytophthora, 0106 biological sciences, ACIDE β-AMINOBUTYRIQUE, Physiology, Arabidopsis, Biology, 01 natural sciences, Aminobutyric acid, 03 medical and health sciences, Immunity, Tobacco, Gene expression, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Vitis, DNA Primers, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Reactive oxygen species, Oxidase test, NADPH oxidase, Reverse Transcriptase Polymerase Chain Reaction, Aminobutyrates, NADPH Oxidases, Hydrogen Peroxide, General Medicine, Kinetics, Enzyme, Biochemistry, chemistry, BABA, biology.protein, Calcium, Signal transduction, Reactive Oxygen Species, Agronomy and Crop Science, RESISTANCE, 010606 plant biology & botany

الوصف: International audience; The molecular mechanisms underlying the process of priming are poorly understood. In the present study, we investigated the early signaling events triggered by β-aminobutyric acid (BABA), a well-known priming-mediated plant resistance inducer. Our results indicate that, in contrast to oligogalacturonides (OG), BABA does not elicit typical defense-related early signaling events nor defense-gene expression in grapevine. However, in OG-elicited cells pretreated with BABA, production of reactive oxygen species (ROS) and expression of the respiratory-burst oxidase homolog RbohD gene were primed. In response to the causal agent of downy mildew Plasmopara viticola, a stronger ROS production was specifically observed in BABA-treated leaves. This process was correlated with an increased resistance. The NADPH oxidase inhibitor diphenylene iodonium (DPI) abolished this primed ROS production and reduced the BABA-induced resistance (BABA-IR). These results suggest that priming of an NADPH oxidase–dependent ROS production contributes to BABA-IR in the Vitis-Plasmopara pathosystem.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff78dfb0f2884b5640592d82d01d0dc0Test
https://doi.org/10.1094/mpmi-23-8-1012Test

المؤلفون: Eduardo Blumwald, Erich Grotewold, Tom Okita, Liwen Jiang, David Wendehenne

المصدر: Plant Science. 266:130

مصطلحات موضوعية: 0106 biological sciences, 0303 health sciences, 03 medical and health sciences, Genetics, Plant Science, General Medicine, 01 natural sciences, Agronomy and Crop Science, 030304 developmental biology, 010606 plant biology & botany

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::d28579cb4f1d7fda52e682b2f30db0b9Test
https://doi.org/10.1016/j.plantsci.2017.09.009Test