Molecular detection and identification of spotted fever group rickettsiae in ticks collected from horses in Cuba
| العنوان: | Molecular detection and identification of spotted fever group rickettsiae in ticks collected from horses in |
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| المؤلفون: | Adrian Alberto Díaz-Sánchez, Belkis Corona-González, Osvaldo Fonseca-Rodríguez, Matthew E. M. Yunik, Evelyn Lobo-Rivero, Neil B. Chilton, Lisset Roblejo-Arias, Roxana Marrero-Perera, Chulantha P. Diyes |
| المصدر: | Medical and Veterinary Entomology. 35:207-212 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | DNA, Bacterial, Nymph, 0301 basic medicine, Ixodidae, 030231 tropical medicine, Zoology, Tick, Polymerase Chain Reaction, 03 medical and health sciences, 0302 clinical medicine, Amblyomma, Animals, Horses, Pathology, Molecular, Rickettsia, Ecology, Evolution, Behavior and Systematics, Dermacentor, Amblyomma mixtum, General Veterinary, biology, Felis, Cuba, Spotted Fever Group Rickettsiosis, 030108 mycology & parasitology, bacterial infections and mycoses, biology.organism_classification, Rickettsia felis, Tick Infestations, Spotted fever, Insect Science, Vector (epidemiology), Arachnid Vectors, Horse Diseases, Parasitology |
| الوصف: | Spotted fever group (SFG) rickettsiae are obligatory intracellular bacteria that cause disease in humans and other animals. Ixodid ticks are the principal vectors of SFG rickettsiae. The present study aimed to determine the prevalence and species identity of SFG rickettsiae in ticks and horses from urban and rural areas of western Cuba using PCR assays. Tick samples, collected from 79 horses, consisted of 14 Amblyomma mixtum adults, 111 Dermacentor nitens adults and 19 pools of D. nitens nymphs (2-5 individuals/pool). The PCR results revealed the presence of Rickettsia spp. in 64% of the A. mixtum adults, 16% of the D. nitens adults, and 11% of the pooled samples of D. nitens nymphs. In contrast, Rickettsia spp. was not detected in any of the 200 horse blood samples included in this study. DNA sequence data of the rickettsial 17 kDa antigen gene showed that Rickettsia amblyommatis was present in A. mixtum; and Rickettsia felis in D. nitens. This is the first report of R. felis in D. nitens in Cuba. The present study extends our knowledge of the potential vector spectrum and distribution of SFG rickettsiae pathogens in western Cuba. |
| تدمد: | 1365-2915 0269-283X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87b2d4b8d66105e3a2753cc3d5446ea0Test https://doi.org/10.1111/mve.12480Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....87b2d4b8d66105e3a2753cc3d5446ea0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652915 0269283X |
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