CXCL10 antagonism and plasma sDPPIV correlate with increasing liver disease in chronic HCV genotype 4 infected patients
| العنوان: | CXCL10 antagonism and plasma sDPPIV correlate with increasing liver disease in chronic HCV genotype 4 infected patients |
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| المؤلفون: | Melissa E. Laird, Khaled Zalata, Darragh Duffy, Dina El. Shenawy, Philippe Bonnard, Armanda Casrouge, Arnaud Fontanet, Mona Rafik, Gamal Esmat, Wagdi Elkashef, Amal Abass, Mostafa Kamal, Rasha Mamdouh, Matthew L. Albert, Abdelhadi M. Shebl, Dina Ragab |
| المساهمون: | Immunobiologie des Cellules Dendritiques, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculty of medicine, Université Ain Shams, Department of Endemic Medicine and Hepatology, Cairo University, CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Université Paris Descartes - Paris 5 (UPD5), This work was a sub study of the ANRS clinical trial 12184 (entitled: ‘‘Liver fibrosis evaluation among HCV genotype 4 infected patients in Egypt’’). It was also supported by the European FP7 project SPHINX (ID 261365), European Project: 261365,EC:FP7:HEALTH,FP7-HEALTH-2010-single-stage,SPHINX(2010), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Service des maladies infectieuses et tropicales [CHU Tenon], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Tenon [APHP], Epidémiologie des Maladies Emergentes, Pasteur-Cnam risques infectieux et émergents (PACRI) |
| المصدر: | Cytokine Cytokine, 2013, 63 (2), pp.105-112. ⟨10.1016/j.cyto.2013.04.016⟩ BASE-Bielefeld Academic Search Engine Cytokine, Elsevier, 2013, 63 (2), pp.105-112. ⟨10.1016/j.cyto.2013.04.016⟩ Cytokine; Vol 63 |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Chemokine, Chronic HCVg4, Hepacivirus, CXCR3, medicine.disease_cause, Biochemistry, Liver disease, 0302 clinical medicine, Immunology and Allergy, DPPIV, 0303 health sciences, biology, CXCL10, virus diseases, Hematology, Hepatitis C, Middle Aged, 3. Good health, Liver, 030220 oncology & carcinogenesis, [SDV.IMM]Life Sciences [q-bio]/Immunology, Egypt, Female, medicine.symptom, Agonist, Adult, Receptors, CXCR3, Adolescent, medicine.drug_class, Hepatitis C virus, Dipeptidyl Peptidase 4, Immunology, Inflammation, 03 medical and health sciences, Young Adult, medicine, Humans, Dipeptidyl-Peptidases and Tripeptidyl-Peptidases, Molecular Biology, 030304 developmental biology, Chemokine antagonism, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Hepatitis C, Chronic, medicine.disease, Lymphocyte Subsets, Chemokine CXCL10, biology.protein, IP10 |
| الوصف: | International audience; Egypt has the highest prevalence of hepatitis C virus infection worldwide. CXCL10 is a potent chemoattractant that directs effector lymphocytes to sites of inflammation. It has been reported that plasma CXCL10 is processed by dipeptidylpeptidase IV (DPPIV) thus leading to the generation of an antagonist form. Using Luminex-based immunoassays we determined the concentration of different forms of CXCL10 (total, agonist, and antagonist). We also evaluated plasma soluble DPPIV (sDPPIV) concentration and plasma dipeptidylpeptidase (DPP) activity. Using flow cytometry and immunohistochemistry, we analyzed the distribution of lymphocyte subsets. Plasma CXCL10 was elevated in chronic HCV patients, however the agonist form was undetectable. Increased sDPPIV concentration and DPP activity supported the NH2-truncation of CXCL10. Finally, we demonstrated an increased frequency of CXCR3(+) cells in the peripheral blood, and low numbers of CXCR3(+) cells within the lobular regions of the liver. These findings generalize the observation of chemokine antagonism as a mechanism of immune modulation in chronic HCV patients and may help guide the use of new therapeutic immune modulators. |
| تدمد: | 1096-0023 1043-4666 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9142cb4761e1fabc868dc3f5f36416ecTest https://pubmed.ncbi.nlm.nih.gov/23664274Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9142cb4761e1fabc868dc3f5f36416ec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10960023 10434666 |
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