Nuclear chromatin patterns in 3 glucocorticoid-resistant RPMI 8226 human myeloma cell sub-lines - Correlations with cell growth and immunological phenotype
| العنوان: | Nuclear chromatin patterns in 3 glucocorticoid-resistant RPMI 8226 human myeloma cell sub-lines - Correlations with cell growth and immunological phenotype |
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| المؤلفون: | Vincent Genty, Victoria El-Khoury, Jean Dufer, Françoise Liautaud-Roger, G. Dine |
| المساهمون: | Inst Biotechnol Troyes, Institue Jean Godinoi, Secteur Prevent, Institue Jean Godinoi, Ecole Centrale Paris, Université de Reims Champagne-Ardenne (URCA), Boughnim, Nabil |
| المصدر: | Cancer Biology and Therapy Cancer Biology and Therapy, Taylor & Francis, 2005, 4 (8), pp.832-839 |
| بيانات النشر: | HAL CCSD, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Cancer Research, Antineoplastic Agents, Hormonal, CD58, Cell, [SDV.CAN]Life Sciences [q-bio]/Cancer, Methylprednisolone, Dexamethasone, Flow cytometry, resistance, 03 medical and health sciences, 0302 clinical medicine, Prophase, image cytometry, [SDV.CAN] Life Sciences [q-bio]/Cancer, Cell Line, Tumor, medicine, Humans, CD44, 030304 developmental biology, Pharmacology, Cell Nucleus, 0303 health sciences, biology, medicine.diagnostic_test, glucocorticoids, Cell growth, nuclear texture, Cell Cycle, Flow Cytometry, Molecular biology, ADP-ribosyl Cyclase 1, Chromatin, medicine.anatomical_structure, Hyaluronan Receptors, Phenotype, Oncology, Cell culture, Doxorubicin, Drug Resistance, Neoplasm, 030220 oncology & carcinogenesis, myeloma cell line, biology.protein, Molecular Medicine, Prednisone, chromatin, Multiple Myeloma, CD38 |
| الوصف: | Nuclear morphological alterations associated with glucocorticoid resistance in human myeloma were evaluated by image cytometry in three human myeloma RPMI 8226 cell sub-lines. Resistance was induced by drug selection using prednisone (8226p), methylprednisolone (8226m) and dexamethasone (8226d), respectively. All these three cell sub lines displayed significant glucocorticoid-resistance without cross-resistance to doxorubicin. Nuclear geometry and texture were analyzed on G0/G1-selected cell nuclei and data compared with cell growth characteristics and membrane expression of CD23, CD38, CD44 and CD58 antigens. When compared to the parental RPMI 8226 cell line, glucocorticoid-resistant cells display a progressive chromatin condensation with heterogeneously distributed large chromatin clumps, a phenomenon not observed in the multidrug-resistant CEM-VLB cells. These alterations were correlated to the resistance index against glucocorticoids and to the expressions of CD38, and of CD44 variant forms CD44v5 and CD44v7-8 antigens. These data suggest that glucocorticoid resistance in RPMI 8226 cells could be associated with sub-visual specific higher-order chromatin organization changes. Furthermore, these alterations are correlated to the expression of membrane markers associated with tumors aggressiveness. |
| اللغة: | English |
| تدمد: | 1538-4047 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c65909206ad61fec6664c9db8a36ea42Test https://hal.archives-ouvertes.fr/hal-00110888Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c65909206ad61fec6664c9db8a36ea42 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15384047 |
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