A comprehensive and comparative phenotypic analysis of the collaborative founder strains identifies new and known phenotypes
| العنوان: | A comprehensive and comparative phenotypic analysis of the collaborative founder strains identifies new and known phenotypes |
|---|---|
| المؤلفون: | Terry Mehaan, Johannes Beckers, Marion Horsch, Thomas Klopstock, Heike Kollmus, Nadine Spielmann, Christoph Lengger, Mouse Phenome Database Team, Manuela A. Östereicher, Jeremy Mason, Juan Antonio Aguilar-Pimentel, Thure Adler, Helmut Fuchs, Wolfgang Wurst, Martin Hrabě de Angelis, Molly A. Bogue, Hamed Haselimashhadi, Eckhard Wolf, Holger Maier, Julia Calzada-Wack, Oana V. Amarie, Wolfgang Hans, Irina Treise, Lillian Garrett, Jochen Graw, Kristin Moreth, Frauke Neff, Valerie Gailus-Durner, Jan Rozman, Dirk H. Busch, Klaus Schughart, Birgit Rathkolb, Rudi Balling, Philipp Mayer-Kuckuk, Ali Önder Yildirim, Sabine M. Hölter, Gregor Miller, Tanja Klein-Rodewald, Arturo Torres, Lore Becker, Ildiko Racz |
| المساهمون: | HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. |
| المصدر: | Mammalian genome 31(1-2), 30-48 (2020). doi:10.1007/s00335-020-09827-3 Mammalian Genome Mamm. Genome 31, 30-48 (2020) Mammalian genome : official journal of the International Mammalian Genome Society |
| بيانات النشر: | Springer, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Collaborative Cross Mice, Male, Genotype, Genetic traits, Quantitative Trait Loci, Mice, Inbred Strains, Phenome, Biology, Quantitative trait locus, Article, 03 medical and health sciences, Mice, 0302 clinical medicine, Phenotypic analysis, Species Specificity, ddc:570, genetics [Collaborative Cross Mice], Databases, Genetic, Genetics, Animals, Genetic Association Studies, 030304 developmental biology, 0303 health sciences, Phenotype, Human genetics, ddc, Blood chemistry, Female, 030217 neurology & neurosurgery, genetics [Mice, Inbred Strains] |
| الوصف: | Myxococcus xanthus DK1622 is known as a proficient producer of different kinds of secondary metabolites (SM) with various biological activities, including myxovirescin A, myxalamide A, myxochromide A and DKxanthene. Low production of SM in the wild type bacteria makes searching for production optimization methods highly desirable. Identification and induction of endogenous key molecular feature(s) regulating the production level of the metabolites remain promising, while heterologous expression of the biosynthetic genes is not always efficient because of various complicating factors including codon usage bias. This study established proteomic and molecular approaches to elucidate the regulatory roles of the ROK regulatory protein in the modification of secondary metabolite biosynthesis. Interestingly, the results revealed that rok inactivation significantly reduced the production of the SM and also changed the motility in the bacteria. Electrophoretic mobility shift assay using purified ROK protein indicated a direct enhancement of the promoters encoding transcription of the DKxanthene, myxochelin A, and myxalamide A biosynthesis machinery. Comparative proteomic analysis by two-dimensional fluorescence difference in-gel electrophoresis (2D-DIGE) was employed to identify the protein profiles of the wild type and rok mutant strains during early and late logarithmic growth phases of the bacterial culture. Resulting data demonstrated overall 130 differently altered proteins by the effect of the rok gene mutation, including putative proteins suspected to be involved in transcriptional regulation, carbohydrate metabolism, development, spore formation, and motility. Except for a slight induction seen in the production of myxovirescin A in a rok over-expression background, no changes were found in the formation of the other SM. From the outcome of our investigation, it is possible to conclude that ROK acts as a pleiotropic regulator of secondary metabolite formation and development in M. xanthus, while its direct effects still remain speculative. More experiments are required to elucidate in detail the variable regulation effects of the protein and to explore applicable approaches for generating valuable SM in this bacterium. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f599d947716ca803a592f30febb81cdTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7f599d947716ca803a592f30febb81cd |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |