A pathway of targeted autophagy is induced by DNA damage in budding yeast
| العنوان: | A pathway of targeted autophagy is induced by DNA damage in budding yeast |
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| المؤلفون: | James E. Haber, Nathan Schiffmann, Robbie Loewith, Silvia G. Chuartzman, Roarke A. Kamber, Amélie Bernard, Vinay V. Eapen, Brenda Lemos, Enrich Sayas, Gonen Memisoglu, Maya Schuldiner, Vladimir Denic, Allison Mazella, Daniel J. Klionsky, Jessie Ang, David P. Waterman |
| المصدر: | Proceedings of the National Academy of Sciences, Vol. 114, No 7 (2017) pp. E1158-E1167 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Programmed cell death, Saccharomyces cerevisiae Proteins, DNA Repair, DNA damage, DNA repair, Saccharomyces cerevisiae, Vesicular Transport Proteins, Autophagy-Related Proteins, Cell Cycle Proteins, Protein Serine-Threonine Kinases, BAG3, 03 medical and health sciences, 0302 clinical medicine, ddc:590, ddc:570, Autophagy, DNA Breaks, Double-Stranded, DNA, Fungal, Checkpoint Kinase 2, Multidisciplinary, biology, fungi, Intracellular Signaling Peptides and Proteins, biology.organism_classification, Cell biology, body regions, 030104 developmental biology, PNAS Plus, Signal transduction, 030217 neurology & neurosurgery, DNA Damage, Signal Transduction |
| الوصف: | Autophagy plays a central role in the DNA damage response (DDR) by controlling the levels of various DNA repair and checkpoint proteins; however, how the DDR communicates with the autophagy pathway remains unknown. Using budding yeast, we demonstrate that global genotoxic damage or even a single unrepaired double-strand break (DSB) initiates a previously undescribed and selective pathway of autophagy that we term genotoxin-induced targeted autophagy (GTA). GTA requires the action primarily of Mec1/ATR and Rad53/CHEK2 checkpoint kinases, in part via transcriptional up-regulation of central autophagy proteins. GTA is distinct from starvation-induced autophagy. GTA requires Atg11, a central component of the selective autophagy machinery, but is different from previously described autophagy pathways. By screening a collection of ∼6,000 yeast mutants, we identified genes that control GTA but do not significantly affect rapamycin-induced autophagy. Overall, our findings establish a pathway of autophagy specific to the DNA damage response. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49cf444b0432f4a8aa5302943ab1cdb0Test https://doi.org/10.1073/pnas.1614364114Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....49cf444b0432f4a8aa5302943ab1cdb0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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