IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual γδT cells in early pregnancy
| العنوان: | IL-25 promotes Th2 bias by upregulating IL-4 and IL-10 expression of decidual γδT cells in early pregnancy |
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| المؤلفون: | Jie Duan, Ying Wang, Yuan Zhang, Ming‑Qing Li, Li‑Ping Jin, Deng‑Xuan Fan |
| المصدر: | Experimental and Therapeutic Medicine |
| بيانات النشر: | Spandidos Publications, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Stromal cell, proliferation, Biology, Immune tolerance, type 2 T helper cells, 03 medical and health sciences, 0302 clinical medicine, Immune system, Immunology and Microbiology (miscellaneous), Interferon, Interleukin 25, medicine, Interleukin 4, γδT cells, Interleukin, Articles, interleukin-25, General Medicine, interleukin-17RB, Interleukin 10, 030104 developmental biology, Cancer research, pregnancy, 030215 immunology, medicine.drug |
| الوصف: | Decidual immune cells (DICs), consisting of both innate and adaptive immune cells, have a pivotal role in maintaining immune tolerance for normal pregnancy. Our previous study demonstrated that interleukin (IL)-25 stimulates the proliferation of decidual stromal cells (DSCs) in an autocrine manner. However, the role of IL-25 in functional regulation of DICs is largely unknown. Flow cytometry was used to analyze the expression of IL-25 and its receptor (IL-17RB) in DICs, and the effect of IL-25 on the expression of Ki-67, IL-4, IL-10, interferon (IFN)-γ and transforming growth factor (TGF)-β in decidual γδT cells. In addition, ELISA assays were performed to detect the secretion of IL-10 and TGF-β in decidual γδT cells. The present findings indicated that decidual CD56 bright CD16-natural killer (NK) cells, natural killer T (NKT) cells, regulatory T (Treg) cells, CD3+ T cells, macrophages and γδT cells co-expressed IL-25 and IL-17RB, particularly γδT cells. Recombinant human (rh) IL-25 protein upregulated the expression of Ki-67, IL-4, and IL-10, but downregulated the expression of IFN-γ in γδT cells; however, anti-human IL-25 or IL-17RB neutralizing antibody reversed these effects. These data suggest that IL-25 may promote IL-10 production by γδT cells as well as the proliferation of γδT cells, and possibly forms a positive feedback loop to maintain a T helper 2 cell bias at the maternal-fetal interface and further contributes to the maintenance of successful pregnancy. |
| تدمد: | 1792-1015 1792-0981 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcfd3c74ca2e6cfb54143647a698d6f0Test https://doi.org/10.3892/etm.2017.5638Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bcfd3c74ca2e6cfb54143647a698d6f0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921015 17920981 |
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