المؤلفون: William Tarnow-Mordi, Bikramjit Das, Lakhbir Kaur Dhaliwal, Venkataseshan Sundaram, Praveen Kumar, Alpana Ghadge

المصدر: Journal of Perinatology. 38:496-504

مصطلحات موضوعية: Pregnancy, medicine.medical_specialty, Cord, business.industry, Obstetrics, Incidence (epidemiology), Obstetrics and Gynecology, Gestational age, medicine.disease, Umbilical cord, law.invention, 03 medical and health sciences, 0302 clinical medicine, medicine.anatomical_structure, Randomized controlled trial, law, 030225 pediatrics, Pediatrics, Perinatology and Child Health, Necrotizing enterocolitis, medicine, Gestation, 030212 general & internal medicine, business

الوصف: To compare effect of placental transfusion by delayed cord clamping (DCC) or cord milking (CM) with early cord clamping (ECC) on a composite of mortality or abnormal neurological status at 40 weeks’ post-menstrual age (PMA) and 24–30 months’ chronological age in neonates of 30–33 weeks’ gestation. Randomized, controlled trial. A composite of mortality or abnormal neurological status at 40 weeks PMA and survival free of neurodevelopmental abnormalities at 24–30 months’ chronological age. A total of 461 neonates were randomized to placental transfusion (n = 233) or to ECC (n = 228). Among those assigned to placental transfusion group, 173 underwent DCC while in the remaining 60, CM was done. Incidence of mortality or abnormal neurological status at 40 weeks PMA (43 (18%) vs 35 (15%), RR (95% CI) 1.2 (0.8, 1.8), p = 0.4) and survival free of neurodevelopmental impairment at 24–30 months of chronological age (99 (47%) vs. 100 (50%); RR (95% CI): 0.9 (0.8, 1.2); P = 0.9) was similar between the study groups. The placental transfusion group showed a trend towards lower incidence of necrotizing enterocolitis. In 30–33 weeks’ gestation preterm neonates, placental transfusion as compared to early cord clamping resulted in similar mortality or abnormal neurological status at 40 weeks PMA and at 24–30 months of chronological age.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::3b3d29d5875eeb6c65caef0345102f8fTest
https://doi.org/10.1038/s41372-018-0064-4Test

المؤلفون: Lakhbir Kaur Dhaliwal, Shalmoli Bhattacharya, Savita Bhunwal, Kanya Mukhopadhyay, Pranab Dey

المصدر: Indian Pediatrics. 55:211-215

مصطلحات موضوعية: Male, medicine.medical_specialty, Birth weight, Chorioamnionitis, Gastroenterology, Cohort Studies, Sepsis, 03 medical and health sciences, 0302 clinical medicine, Pregnancy, Risk Factors, 030225 pediatrics, Internal medicine, Ductus arteriosus, medicine, Birth Weight, Humans, Rupture of membranes, Bronchopulmonary Dysplasia, 030219 obstetrics & reproductive medicine, business.industry, Infant, Newborn, medicine.disease, medicine.anatomical_structure, Bronchopulmonary dysplasia, Pediatrics, Perinatology and Child Health, Gestation, Female, business, Infant, Premature

الوصف: To find out the incidence and associations of bronchopulmonary dysplasia (BPD) in preterm neonates. Descriptive cohort. All consecutively born neonates

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e17f47af500f9c8c68ba2280d11fb2aTest
https://doi.org/10.1007/s13312-018-1319-zTest

المؤلفون: Shalini Gainder, Sunil Sethi, Lakhbir Kaur Dhaliwal, Nidhi Jindal

المصدر: The Journal of Obstetrics and Gynecology of India. 68:123-128

مصطلحات موضوعية: 0301 basic medicine, Gynecology, Infertility, medicine.medical_specialty, 030219 obstetrics & reproductive medicine, medicine.diagnostic_test, business.industry, 030106 microbiology, Obstetrics and Gynecology, Diagnostic dilemma, Genital tuberculosis, medicine.disease, Secondary infertility, Diagnostic modalities, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Medicine, Original Article, Mycobacteria growth indicator tube, Detection rate, business, Endometrial biopsy

الوصف: BACKGROUND: The purpose of this study was to assess the utility of Mycobacteria Growth Indicator Tube (MGIT) 960 culture medium for the diagnosis of genital tuberculosis (GTB) in women presenting with infertility. METHODS: The premenstrual endometrial biopsy samples in 300 women presenting with primary and secondary infertility were subjected to AFB smear method, histopathological examination and culture on LJ and MGIT 960 media. Detection rates were compared for diagnostic modalities and their combinations. RESULTS: In total, 30 cases were positive for genital tuberculosis by either of the four tests employed. The detection rates for AFB smear, MGIT culture, LJ culture and HPE were 50, 46.7, 3.3 and 33.3%, respectively. A combination of smear examination for AFB, MGIT 960 culture and histopathological examination was able to detect all the positive cases. A combination of MGIT and LJ media provided no added advantage over MGIT alone since the only case where LJ culture was positive had been detected by positive MGIT culture. In as many as five positive cases (16.7%), only MGIT culture was positive. CONCLUSION: The addition of MGIT 960 culture medium to routine battery of investigations in infertility patients significantly improves the diagnosis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be1ba371d8935096b746390faa587468Test
https://doi.org/10.1007/s13224-017-1077-1Test

المؤلفون: Priyanka Bhagat, Nalini Gupta, Ruchita Tyagi, Arvind Rajwanshi, Lakhbir Kaur Dhaliwal, Bhavna Rai, Shalini Gainder

المصدر: Journal of Cytology
Journal of Cytology, Vol 34, Iss 2, Pp 95-100 (2017)

مصطلحات موضوعية: Pathology, medicine.medical_specialty, Histology, Gynecologic oncology, Pathology and Forensic Medicine, 03 medical and health sciences, 0302 clinical medicine, Cytology, Dysgerminoma, Medicine, liquid-based cytology, Endometrioid adenocarcinoma, papillary serous carcinoma, peritoneal wash, lcsh:QH573-671, lcsh:Cytology, business.industry, 030224 pathology, medicine.disease, Peritoneal washing, 030220 oncology & carcinogenesis, Liquid-based cytology, Clear cell carcinoma, Original Article, Teratoma, Mucinous cystadenocarcinoma, business

الوصف: Context: Peritoneal washing is performed for staging of gynecologic tumors to detect subclinical intraperitoneal metastases. Aim: The aim of the present study was to assess the impact of SurePathTM liquid-based cytology (LBC) in peritoneal washing in various gynecological malignancies. Settings and Design: An audit of peritoneal-fluid/washing (January 2012 to July 2013) was performed with corresponding gynecologic specimens. All peritoneal washings were processed using both conventional and LBC technique. Suspicious cases on cytology were reported along with gynecologic specimens. Results: There were a total of 393 peritoneal fluids. Eighty-three (21.1%) were positive for malignancy, and the corresponding histology was available in 352 (89.6%) cases. Sixty-nine positive samples had ovarian malignancies and 5 had uterine causes. There were 9 cases of peritoneal washings in which no histopathology was available. The most common cause of positive peritoneal cytology was ovarian serous carcinoma in 55/84 (65.5%) cases. Other causes included mucinous cystadenocarcinoma, dysgerminoma, squamous cell carcinoma in teratoma, yolk sac tumor, and granulosa cell tumor. Uterine causes included 2/45 (4.4%) cases of endometrioid adenocarcinoma, ¼ (25%) cases of clear cell carcinoma, ½ (50%) cases of carcinosarcoma, and ¼ (25%) cervix carcinoma. On review of positive cases (n = 83), 10 cases were identified, which had nil (n = 4) to low cellularity (

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6935d820a9d3faa10f65184c68263933Test
http://europepmc.org/articles/PMC5398027Test

المؤلفون: Neelam Aggarwal, Vanita Suri, Lakhbir Kaur Dhaliwal, Rimpi Singla

المصدر: Bangladesh Journal of Obstetrics & Gynaecology. 30:25-29

مصطلحات موضوعية: medicine.medical_specialty, Pregnancy, Referral, business.industry, Obstetrics and Gynecology, Mean age, 030206 dentistry, Audit, North india, medicine.disease, Tertiary care, Health care delivery, 03 medical and health sciences, 0302 clinical medicine, Family medicine, Health care, Medicine, 030212 general & internal medicine, business

الوصف: Objective (s):The aim of the study was to find out the characteristics of emergency obstetric referrals, to evaluate the lapses in the existing referral system and to suggest the plausible solutions to improve the obstetric health care delivery system in India.Materials and methods: This prospective descriptive study was carried out in the Department of Obstertrics and Gynaecology PGIMER Chandigarh India from September 2012 to December 2012.All the obstetric emergency referrals to our institute, a tertiary care centre in North India were critically reviewed for reason for referral, adequacy of care provided before referral, lapses in management, factors behind suboptimal care and remedial measures are suggested.Results: There were 232 emergency referrals available for analysis. Of these 76% patients were referred directly to the institute, the highest level in referral chain in the region. Mean age was 26yrs, 28.6% of the patients were illiterate. In 75.5% of cases, the management of obstetric emergency before referral was suboptimal or poor. In 16% of cases patient failed to seek medical help timely despite emergency. In 40% of cases there was undue delay in referring or failure to provide even the basic care. Anticipated premature delivery and hypertensive disorders of pregnancy were the most common diagnosis at referral.Conclusion: Results from this pilot study shows that in spite of the most extensive health care infrastructure and so- called hierarchal referral system, there is gross inadequacy in referral system in India.Bangladesh J Obstet Gynaecol, 2015; Vol. 30(1) : 25-29

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::a1e99fac4a030165c813c04bc7fe3ae4Test
https://doi.org/10.3329/bjog.v30i1.30504Test

المؤلفون: Santosh Kurra, B. S. Dhillon, Gurjit Kaur, D.N. Rao, Lakhbir Kaur Dhaliwal, Suneeta Mittal, Neeta Kumar, Hari Shankar, Nomita Chandhiok, Rajat Sandhir

المصدر: Saudi Journal of Biological Sciences, Vol 23, Iss 6, Pp 724-730 (2016)

مصطلحات موضوعية: Pediatrics, medicine.medical_specialty, Iron status markers, Iron+folic acid, Iron folic acid supplementation, Anaemia, law.invention, 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, Obstetrics and gynaecology, Pregnancy, law, Statistical significance, medicine, lcsh:QH301-705.5, Agricultural and Biological Sciences(all), business.industry, Obstetrics, Iron deficiency, medicine.disease, lcsh:Biology (General), 030220 oncology & carcinogenesis, Original Article, New delhi, Iron status, General Agricultural and Biological Sciences, business, 030215 immunology

الوصف: Anaemia during pregnancy is most commonly observed and highly prevalent in South-East Asia. Various effective programmes have been laid down for its management, mainly daily supplementation of iron folic acid (IFA) tablets. Following the same, standard obstetrical practice has included the IFA supplementation without requiring the determination of iron deficiency. In this study, a total of 120 primigravida (N = 60; non-anaemic (Hb > 11 g/dl) and N = 60 anaemic (Hb = 8–11 g/dl)) were selected among those attending the Antenatal Clinic in Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India. They were supplemented with daily and weekly IFA tablets till 6 weeks postpartum. Corresponding changes in haemoglobin level on advance of pregnancy, side effects and compliance associated with daily and weekly IFA supplementation and its associations with iron status markers were studied. The inflammatory markers were also estimated. The statistical significance level (p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a25c00e851fceed01a2e2f4fe542af5eTest
https://doi.org/10.1016/j.sjbs.2015.09.007Test

المؤلفون: Neha Mittal, Upasana Gautam, Radhika Srinivasan, Arvind Rajwanshi, Raje Nijhawan, Swati Sood, Nalini Gupta, Lakhbir Kaur Dhaliwal

المصدر: Indian Journal of Pathology and Microbiology, Vol 59, Iss 4, Pp 481-488 (2016)

مصطلحات موضوعية: Microbiology (medical), Adult, Pathology, medicine.medical_specialty, endocrine system diseases, Serous carcinoma, serous tubal intraepithelial carcinoma, lcsh:QR1-502, Context (language use), Biology, Fallopian tube, sectioning and extensively examining the fimbria protocol, lcsh:Microbiology, Pathology and Forensic Medicine, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Fallopian Tube Neoplasm, medicine, lcsh:Pathology, Fallopian Tube Neoplasms, Humans, Prospective Studies, Cystadenocarcinoma, Fallopian Tubes, secretory cell outgrowths, 030219 obstetrics & reproductive medicine, Carcinoma in situ, Serous Tubal Intraepithelial Carcinoma, General Medicine, Middle Aged, medicine.disease, female genital diseases and pregnancy complications, p53 signature, Cystadenocarcinoma, Serous, Serous fluid, medicine.anatomical_structure, serous carcinoma, 030220 oncology & carcinogenesis, Case-Control Studies, Female, Tumor Suppressor Protein p53, Carcinoma in Situ, lcsh:RB1-214

الوصف: Context: High-grade serous carcinomas of ovarian, tubal, and peritoneal origin are together referred as pelvic serous carcinoma. The fallopian tubes, ovarian surface epithelium, and the tuboperitoneal junctional epithelium are all implicated in pelvic serous carcinogenesis. Aims: The aim of this study is to identify putative precursor lesions of serous carcinoma including secretory cell outgrowths (SCOUTs), serous tubal intraepithelial carcinoma (STIC), and p53 signatures and assign its probable site of origin. Settings and Design: Prospective case-control study of consecutive specimen comprising 32 serous carcinomas and 31 controls (10 normal adnexa, 10 benign and 6 atypically proliferative surface epithelial tumors, and 5 other carcinomas). Subjects and Methods: Sectioning and extensive examination of the fimbrial end (SEE-FIM) protocol along with immunohistochemistry for Bcl-2, p53, and Ki-67 was employed for evaluating invasive carcinoma and precursor lesions in cases versus controls. Results: SCOUT, p53 signatures, and STIC were most frequent in the serous carcinomas. p53 signatures and STIC were always seen in the fimbrial end. STICs were exclusively present in serous carcinomas, more common in ipsilateral tubes of cases with dominant ovarian mass. Multifocal p53 signatures with STIC were seen in 7 (21.9%) cases. STIC was present with or without an invasive carcinoma in 25% and in 6.25% of cases of pelvic serous carcinomas, respectively. The junctional epithelia did not show any lesion in any group. Conclusions: SEE-FIM protocol is recommended for evaluation of sporadicpelvic (ovarian/tubal/peritoneal) serous carcinoma. Based on the presence of STIC or invasive carcinoma, nearly 60% of all pelvic serous carcinomas are of fallopian tubal origin.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90c221bc1ea06f7232a3cccf358b6fdeTest
http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2016;volume=59;issue=4;spage=481;epage=488;aulast=MittalTest

المؤلفون: Naresh Sachdeva, Anil Bhansali, Mahinder Paul, Lakhbir Kaur Dhaliwal, Devi Dayal

المصدر: Cytotherapy. 20(11)

مصطلحات موضوعية: 0301 basic medicine, Adult, CD4-Positive T-Lymphocytes, Cancer Research, Immunology, Cell- and Tissue-Based Therapy, chemical and pharmacologic phenomena, Stimulation, medicine.disease_cause, T-Lymphocytes, Regulatory, Autoimmunity, Umbilical Cord, Cell therapy, Immunomodulation, 03 medical and health sciences, 0302 clinical medicine, Transforming Growth Factor beta, medicine, Immunology and Allergy, Humans, IL-2 receptor, Genetics (clinical), Cells, Cultured, Proinsulin, Transplantation, Chemistry, Infant, Newborn, Interleukin-2 Receptor alpha Subunit, hemic and immune systems, Forkhead Transcription Factors, Cell Biology, Fetal Blood, In vitro, 030104 developmental biology, Diabetes Mellitus, Type 1, Oncology, Cord blood, Female, 030215 immunology, Transforming growth factor

الوصف: Background Antigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D). Cord blood (CB) can serve as an efficient and safe source for Tregs for antigen-specific immunomodulation in T1D, a strategy that is yet to be explored. Therefore, we assessed the potential of CB in generation of proinsulin (PI)-specific Tregs by using HLA class II tetramers. Methods We analyzed the frequency of PI-specific natural Tregs (nTregs) and induced Tregs (iTregs) derived from the CB as well as peripheral blood (PB) of patients with T1D and healthy control subjects. For this, CD4+CD25+CD127low and CD4+CD25-T cells were cultured in the presence of PI-derived peptides, transforming growth factor (TGF)-β and rapamycin. PI-specific Tregs were then selected using allele-specific HLA II tetramers loaded with PI-derived peptides, followed by suppression assays. Results Following stimulation, we observed that CB harbors a significantly higher frequency of PI-specific Tregs than PB of subjects with T1D (P = 0.0003). Further, the proportion of PI-specific Tregs was significantly higher in both the nTreg (P = 0.01) and iTreg (P = 0.0003) compartments of CB as compared with PB of subjects with T1D. In co-culture experiments, the PI-specific Tregs suppressed the proliferation of effector T cells significantly (P = 0.0006). The expanded nTregs were able to retain hypomethylation status at their Tregs-specific demethylated region (TSDR), whereas iTregs were unable to acquire the characteristic demethylation pattern. Conclusion Our study demonstrates that CB can serve as an excellent source for generation of functional antigen-specific Tregs for immunotherapeutic approaches in subjects with T1D.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1d1296747742f727c916606f34c638dTest
https://pubmed.ncbi.nlm.nih.gov/30340983Test

المؤلفون: Lakhbir Kaur Dhaliwal, Kanhaiya Agrawal, Shalini Gainder, Vanita Suri

المصدر: Journal of Human Reproductive Sciences
Journal of Human Reproductive Sciences, Vol 10, Iss 4, Pp 261-264 (2017)

مصطلحات موضوعية: medicine.medical_specialty, medicine.medical_treatment, media_common.quotation_subject, Urology, stair-step protocol, lcsh:Gynecology and obstetrics, law.invention, 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, law, medicine, In patient, 030212 general & internal medicine, Ovulation, lcsh:RG1-991, media_common, polycystic ovary, Pregnancy, 030219 obstetrics & reproductive medicine, business.industry, Clomiphene citrate, medicine.disease, Polycystic ovary, Effective dose (pharmacology), Regimen, Reproductive Medicine, Ovulation induction, Original Article, ovulation induction, business

الوصف: Objective: To determine the efficacy of the stair-step protocol (SSP) using clomiphene citrate (CC) in patients with polycystic ovary syndrome (PCOS) and compare it with traditional regimen. Design: This was randomized control trial. Setting: Infertility Clinic. Patient(s): Sixty infertile PCOS women. Intervention(s): Patients were randomized into the study (SSP – 30 patients) and control group (traditional protocol – 30 patients). In the SSP, patients were treated with CC 50 mg/day for 5 days and in nonresponsive patients, the dosage was increased to 100 mg/day for 5 days in the same cycle. Maximum dose of 150 mg was given until the dominant follicle was generated. In control group, the dose increment in nonovulatory cases was done in subsequent cycle. Ultrasonography follow-up was done to detect ovulation. Main Outcome Measure(s): Ovulation rate and duration of treatment. Results: Ovulation (66.7% vs. 50% respectively) and pregnancy rates (26.7% vs. 15.7%) were similar between the stair step and the control group. The duration of treatment was significantly shorter in stair step compared to traditional protocol (17.23 vs. 53 days). CC 100 mg was the most effective dose for ovulation in either group. There were no significant differences in the systemic side effect. Conclusions: By using SSP, effective treatment is provided in significantly shorter time period without any detrimental effect on the ovulation and pregnancy rates.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14786ba90bff41901ba59e28467e907dTest
https://pubmed.ncbi.nlm.nih.gov/29430152Test

المؤلفون: Rashmi Bagga, Sanjeev Sharma, Shifa Javed, Bal Krishan Sharma, Swati Sood, Shalmoli Bhattacharyya, Lakhbir Kaur Dhaliwal, Radhika Srinivasan, Charan Singh Rayat

المصدر: BMC Cancer
BMC Cancer, Vol 18, Iss 1, Pp 1-12 (2018)

مصطلحات موضوعية: 0301 basic medicine, HPV16, Cancer Research, Epithelial-Mesenchymal Transition, Biopsy, Population, Karyotype, Uterine Cervical Neoplasms, Antineoplastic Agents, Minisatellite Repeats, Biology, Low passage, lcsh:RC254-282, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, Cancer stem cell, Cell Line, Tumor, Genetics, medicine, Humans, Epithelial–mesenchymal transition, AC133 Antigen, CD133, Cell Self Renewal, education, Cervical cancer, education.field_of_study, Human papillomavirus 16, medicine.diagnostic_test, Cancer stem cells, Cell Cycle, Papillomavirus Infections, Cell cycle, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, Flow Cytometry, 030104 developmental biology, Oncology, Cell culture, 030220 oncology & carcinogenesis, Cancer research, Neoplastic Stem Cells, Adenocarcinoma, Cell lines, Female, Cisplatin, Research Article

الوصف: Background Cervical cancer is a major cause of cancer-related mortality in women in the developing world. Cancer Stem cells (CSC) have been implicated in treatment resistance and metastases development; hence understanding their significance is important. Methods Primary culture from tissue biopsies of invasive cervical cancer and serial passaging was performed for establishing cell lines. Variable Number Tandem Repeat (VNTR) assay was performed for comparison of cell lines with their parental tissue. Tumorsphere and Aldefluor assays enabled isolation of cancer stem cells (CSC); immunofluorescence and flow cytometry were performed for their surface phenotypic expression in cell lines and in 28 tissue samples. Quantitative real-time PCR for stemness and epithelial-mesenchymal transition (EMT) markers, MTT cytotoxicity assay, cell cycle analysis and cell kinetic studies were performed. Results Four low-passage novel cell lines designated RSBS-9, − 14 and − 23 from squamous cell carcinoma and RSBS-43 from adenocarcinoma of the uterine cervix were established. All were HPV16+. VNTR assay confirmed their uniqueness and derivation from respective parental tissue. CSC isolated from these cell lines showed CD133+ phenotype. In tissue samples of untreated invasive cervical cancer, CD133+ CSCs ranged from 1.3–23% of the total population which increased 2.8-fold in radiation-resistant cases. Comparison of CD133+ with CD133− bulk population cells revealed increased tumorsphere formation and upregulation of stemness and epithelial-mesenchymal transition (EMT) markers with no significant difference in cisplatin sensitivity. Conclusion Low-passage cell lines developed would serve as models for studying tumor biology. Cancer Stem Cells in cervical cancer display CD133+ phenotype and are increased in relapsed cases and hence should be targeted for achieving remission. Electronic supplementary material The online version of this article (10.1186/s12885-018-4237-5) contains supplementary material, which is available to authorized users.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::881722c58dca4a1e2f1ff97c4464c018Test
https://pubmed.ncbi.nlm.nih.gov/29609538Test