Cell-associated hiv-1 unspliced-to-multiply-spliced rna ratio at 12 weeks of art predicts immune reconstitution on therapy
| العنوان: | Cell-associated hiv-1 unspliced-to-multiply-spliced rna ratio at 12 weeks of art predicts immune reconstitution on therapy |
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| المؤلفون: | Alexander O. Pasternak, Ben Berkhout, Neeltje A. Kootstra, Mirte Scherpenisse, Margreet Bakker |
| المساهمون: | Experimental Immunology, AII - Infectious diseases, APH - Aging & Later Life, Medical Microbiology and Infection Prevention |
| المصدر: | mBio, 12(2):e00099-21, 1-25. American Society for Microbiology mBio, Vol 12, Iss 2 (2021) mBio |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, CD4-Positive T-Lymphocytes, Male, Time Factors, Sustained Virologic Response, Cell-associated RNA, Cell, HIV Infections, Lymphocyte Activation, Microbiology, 03 medical and health sciences, Viral reservoirs, 0302 clinical medicine, Immune system, Acquired immunodeficiency syndrome (AIDS), Antiretroviral Therapy, Highly Active, Virology, Humans, Medicine, Longitudinal Studies, 030212 general & internal medicine, Retrospective Studies, 030304 developmental biology, 0303 health sciences, business.industry, Human immunodeficiency virus, RNA, Viral Load, Immune reconstitution, medicine.disease, QR1-502, AIDS, medicine.anatomical_structure, Anti-Retroviral Agents, Viral replication, Apoptosis, Viral persistence, Cohort, Immunology, HIV-1, RNA, Viral, Biomarker (medicine), business, Biomarkers, Research Article |
| الوصف: | Human immunodeficiency virus (HIV) infection is currently managed by antiretroviral drugs, which block virus replication and promote immune restoration. However, the latter effect is not universal, with a proportion of infected individuals failing to sufficiently reconstitute their immune function despite a successful virological response to antiretroviral therapy (ART). Incomplete restoration of CD4+ T-cell counts on antiretroviral therapy (ART) is a major predictor of HIV-related morbidity and mortality. To understand the possible mechanisms behind this poor immunological response despite viral suppression, we longitudinally measured more than 50 virological and immunological biomarkers in a cohort of HIV-infected individuals at several time points during the first 96 weeks of virologically suppressive ART. No baseline virological or immunological marker was predictive of the degree of immune reconstitution. However, the cell-associated HIV-1 unspliced-to-multiply-spliced (US/MS) RNA ratio at 12 weeks of ART positively correlated with markers of CD4+ T-cell activation and apoptosis and negatively predicted both the absolute and relative CD4+ T-cell counts at 48 and 96 weeks. A higher US/MS RNA ratio may reflect the higher frequency of productively infected cells that could exert pressure on the immune system, contributing to persistent immune activation and apoptosis and subsequently to a poor immunological response to ART. |
| اللغة: | English |
| تدمد: | 2161-2129 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a91046dc4350a988f8178786c7acdd33Test https://pure.amc.nl/en/publications/cellassociated-hiv1-unsplicedtomultiplyspliced-rna-ratio-at-12-weeks-of-art-predicts-immune-reconstitution-on-therapyTest(efe3b063-5f73-48c8-aa96-54c51a283ce4).html |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a91046dc4350a988f8178786c7acdd33 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21612129 |
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