Capsaicin Targets Lipogenesis in HepG2 Cells Through AMPK Activation, AKT Inhibition and PPARs Regulation
| العنوان: | Capsaicin Targets Lipogenesis in HepG2 Cells Through AMPK Activation, AKT Inhibition and PPARs Regulation |
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| المؤلفون: | Inés Díaz-Laviada, Pedro A. Mateos-Gómez, Nieves Rodríguez-Henche, Belén G. Sánchez, Alicia Bort |
| المساهمون: | Universidad de Alcalá. Departamento de Biología de Sistemas |
| المصدر: | International Journal of Molecular Sciences, Vol 20, Iss 7, p 1660 (2019) e_Buah Biblioteca Digital Universidad de Alcalá instname International Journal of Molecular Sciences Volume 20 Issue 7 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, AMPK, PPARγ, Peroxisome Proliferator-Activated Receptors, AMP-Activated Protein Kinases, Pharmacology, capsaicin, PPARα, lcsh:Chemistry, chemistry.chemical_compound, 0302 clinical medicine, lipid metabolism, lcsh:QH301-705.5, Spectroscopy, Chemistry, Hep G2 Cells, General Medicine, Lipids, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Up-Regulation, Computer Science Applications, Lipogenesis, lipids (amino acids, peptides, and proteins), medicine.drug, HepG2, PPARx, PPARy, 030209 endocrinology & metabolism, Models, Biological, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, Autophagy, medicine, Humans, Physical and Theoretical Chemistry, Molecular Biology, Protein kinase B, Biology, PI3K/AKT/mTOR pathway, Biología y Biomedicina, AKT, Organic Chemistry, Troglitazone, Lipid metabolism, Enzyme Activation, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Capsaicin, Proto-Oncogene Proteins c-akt |
| الوصف: | Obesity, a major risk factor for chronic diseases such as type 2 diabetes (T2D), represents a serious primary health problem worldwide. Dietary habits are of special interest to prevent and counteract the obesity and its associated metabolic disorders, including lipid steatosis. Capsaicin, a pungent compound of chili peppers, has been found to ameliorate diet-induced obesity in rodents and humans. The purpose of this study was to examine the effect of capsaicin on hepatic lipogenesis and to delineate the underlying signaling pathways involved, using HepG2 cells as an experimental model. Cellular neutral lipids, stained with BODIPY493/503, were quantified by flow cytometry, and the protein expression and activity were determined by immunoblotting. Capsaicin reduced basal neutral lipid content in HepG2 cells, as well that induced by troglitazone or by oleic acid. This effect of capsaicin was prevented by dorsomorphin and GW9662, pharmacological inhibitors of AMPK and PPAR&gamma respectively. In addition, capsaicin activated AMPK and inhibited the AKT/mTOR pathway, major regulators of hepatic lipogenesis. Furthermore, capsaicin blocked autophagy and increased PGC-1&alpha protein. These results suggest that capsaicin behaves as an anti-lipogenic compound in HepG2 cells. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a582b6f69e24e3c93f8d47ff6f987a91Test https://www.mdpi.com/1422-0067/20/7/1660Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a582b6f69e24e3c93f8d47ff6f987a91 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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