A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells
| العنوان: | A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells |
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| المؤلفون: | Claire Mamelonet, Stéphane Audebert, Brice Chanez, Joëlle Salameh, Kevin Ostacolo, Luc Camoin, Emilie Baudelet, Sylvie Thuault, Danièle Salaün, Ali Badache |
| المساهمون: | Bodescot, Myriam, Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie et traitement des maladies du foie, Université Paris-Sud - Paris 11 (UP11)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Iceland [Reykjavik], Department of Medical Oncology [Marseille], Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Marseille Proteomics [Marseille], Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut Paoli-Calmettes, This work was supported by Canceropôle Provence-Alpes-Côte d’Azur (PACA), Institut National du Cancer, Conseil Régional PACA and Site de Recherche Intégrée sur le Cancer (SIRIC). Proteomic analyses were performed at the mass spectrometry facility of Marseille Proteomics supported by IBISA (Infrastructures Biologie Santé et Agronomie), Plateforme Technologique Aix-Marseille, Canceropôle PACA, Région Sud Provence-Alpes-Côte d’Azur, Fonds Européen de Développement Régional (FEDER) and Plan Cancer., Canceropôle Provence-Alpes-Côte d’Azur (PACA), Institut National du Cancer, Conseil Régional PACA and Site de Recherche Intégrée sur le Cancer (SIRIC), Læknadeild (HÍ), Faculty of Medicine (UI), Lífvísindasetur (HÍ), Biomedical Center (UI), Heilbrigðisvísindasvið (HÍ), School of Health Sciences (UI), Háskóli Íslands, University of Iceland, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Badache, Ali |
| المصدر: | Scientific Reports Scientific Reports, 2020, 10 (1), pp.6787. ⟨10.1038/s41598-020-63926-4⟩ Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-020-63926-4⟩ Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.6787. ⟨10.1038/s41598-020-63926-4⟩ Scientific Reports, 2020, 10 (1), ⟨10.1038/s41598-020-63926-4⟩ |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Proteomics, [SDV]Life Sciences [q-bio], lcsh:Medicine, Interactome, Mass Spectrometry, 0302 clinical medicine, Breast cancer, Brjóstakrabbamein, Carbon-Nitrogen Ligases, Protein Interaction Maps, lcsh:Science, Cancer, chemistry.chemical_classification, 0303 health sciences, Escherichia coli Proteins, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], Protein-protein interaction networks, 3. Good health, [SDV] Life Sciences [q-bio], Frumulíffræði, 030220 oncology & carcinogenesis, Biotinylation, Podosomes, Invadopodia, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Protein Binding, Cell biology, Recombinant Fusion Proteins, [SDV.CAN]Life Sciences [q-bio]/Cancer, Computational biology, Biology, Article, Actin cytoskeleton organization, 03 medical and health sciences, Interstitial matrix, [SDV.CAN] Life Sciences [q-bio]/Cancer, Cell Line, Tumor, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Humans, 030304 developmental biology, DNA ligase, lcsh:R, PX domain, Repressor Proteins, Adaptor Proteins, Vesicular Transport, chemistry, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], Protein–protein interaction networks, lcsh:Q, Breast cancer cells |
| الوصف: | Publiher's version (útgefin grein) Metastatic progression is the leading cause of mortality in breast cancer. Invasive tumor cells develop invadopodia to travel through basement membranes and the interstitial matrix. Substantial efforts have been made to characterize invadopodia molecular composition. However, their full molecular identity is still missing due to the difficulty in isolating them. To fill this gap, we developed a non-hypothesis driven proteomic approach based on the BioID proximity biotinylation technology, using the invadopodia-specific protein Tks5α fused to the promiscuous biotin ligase BirA* as bait. In invasive breast cancer cells, Tks5α fusion concentrated to invadopodia and selectively biotinylated invadopodia components, in contrast to a fusion which lacked the membrane-targeting PX domain (Tks5β). Biotinylated proteins were isolated by affinity capture and identified by mass spectrometry. We identified known invadopodia components, revealing the pertinence of our strategy. Furthermore, we observed that Tks5 newly identified close neighbors belonged to a biologically relevant network centered on actin cytoskeleton organization. Analysis of Tks5β interactome demonstrated that some partners bound Tks5 before its recruitment to invadopodia. Thus, the present strategy allowed us to identify novel Tks5 partners that were not identified by traditional approaches and could help get a more comprehensive picture of invadopodia molecular landscape. We thank D. Isnardon and M. Rodriguez (CRCM Microscopy Platform) for support and S.A. Courtneidge, K.J. Roux and C. Lachaud for sharing reagents. This work was supported by Canceropôle Provence-Alpes-Côte d’Azur (PACA), Institut National du Cancer, Conseil Régional PACA and Site de Recherche Intégrée sur le Cancer (SIRIC). Proteomic analyses were performed at the mass spectrometry facility of Marseille Proteomics supported by IBISA (Infrastructures Biologie Santé et Agronomie), Plateforme Technologique Aix-Marseille, Canceropôle PACA, Région Sud Provence-Alpes-Côte d’Azur, Fonds Européen de Développement Régional (FEDER) and Plan Cancer. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::580bd552db30dffcf89615758078e202Test https://doi.org/10.1038/s41598-020-63926-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....580bd552db30dffcf89615758078e202 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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