Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture
| العنوان: | Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture |
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| المؤلفون: | Myong-Chol Lee, Min-Jen Tseng, Ya-Hui Chi, Yu-Cheng Huang, Min-Lang Huang, Zhao-Ying Ding, Ying-Hsuan Wang |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | Rockefeller University Press, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Genotype, Nuclear Envelope, LINC complex, Transfection, Article, Cell Line, Animals, Genetically Modified, 03 medical and health sciences, Transduction (genetics), 0302 clinical medicine, Ovarian Follicle, Animals, Drosophila Proteins, Humans, Cytoskeleton, Research Articles, Regulation of gene expression, biology, Gene Expression Regulation, Developmental, Membrane Proteins, Cell Biology, biology.organism_classification, Cell biology, Drosophila melanogaster, Phenotype, 030104 developmental biology, Microscopy, Fluorescence, Cytoplasm, Multiprotein Complexes, Female, Signal transduction, 030217 neurology & neurosurgery, Signal Transduction |
| الوصف: | LINC complexes connect the inner and outer nuclear membrane (ONM) to transduce nucleocytoskeletal force. Ding et al. identify an ONM protein, Kuduk/TMEM258, which modulates the quality of LINC complexes and regulates the nuclear envelope architecture, nuclear positioning, and the development of ovarian follicles. Linker of nucleoskeleton and cytoskeleton (LINC) complexes spanning the nuclear envelope (NE) contribute to nucleocytoskeletal force transduction. A few NE proteins have been found to regulate the LINC complex. In this study, we identify one, Kuduk (Kud), which can reside at the outer nuclear membrane and is required for the development of Drosophila melanogaster ovarian follicles and NE morphology of myonuclei. Kud associates with LINC complex components in an evolutionarily conserved manner. Loss of Kud increases the level but impairs functioning of the LINC complex. Overexpression of Kud suppresses NE targeting of cytoskeleton-free LINC complexes. Thus, Kud acts as a quality control mechanism for LINC-mediated nucleocytoskeletal connections. Genetic data indicate that Kud also functions independently of the LINC complex. Overexpression of the human orthologue TMEM258 in Drosophila proved functional conservation. These findings expand our understanding of the regulation of LINC complexes and NE architecture. |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7e707fd362ee85737fdfebcf540a2cdTest https://doi.org/10.1083/jcb.201606043Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c7e707fd362ee85737fdfebcf540a2cd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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