Analysis of the immunoglobulin G (IgG) secretion efficiency in recombinant Chinese hamster ovary (CHO) cells by using Citrine-fusion IgG
| العنوان: | Analysis of the immunoglobulin G (IgG) secretion efficiency in recombinant Chinese hamster ovary (CHO) cells by using Citrine-fusion IgG |
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| المؤلفون: | Kohei Kaneyoshi, Takeshi Omasa, Keiji Uchiyama, Noriko Yamano-Adachi, Yuichi Koga |
| المصدر: | Cytotechnology. 71:193-207 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, biology, Chemistry, Chinese hamster ovary cell, Clinical Biochemistry, Biomedical Engineering, Secretory Processes, Bioengineering, Endogeny, Cell Biology, Molecular biology, Article, Immunoglobulin G, law.invention, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Secretory protein, law, 030220 oncology & carcinogenesis, biology.protein, Recombinant DNA, Secretion, Intracellular, Biotechnology |
| الوصف: | Biopharmaceuticals represented by immunoglobulin G (IgG) are produced by the cultivation of recombinant animal cells, especially Chinese hamster ovary (CHO) cells. It is thought that the intracellular secretion process of IgG is a bottleneck in the production of biopharmaceuticals. Many studies on the regulation of endogenous secretory protein expression levels have shown improved productivity. However, these strategies have not universally improved the productivity of various proteins. A more rational and efficient establishment of high producer cells is required based on an understanding of the secretory processes in IgG producing CHO cells. In this study, a CHO cell line producing humanized IgG1, which was genetically fused with fluorescent proteins, was established to directly analyze intracellular secretion. The relationship between the amount of intracellular and secreted IgG was analyzed at the single cell level by an automated single-cell analysis and isolation system equipped with dual color fluorescent filters. The amounts of intracellular and secreted IgG showed a weak positive correlation. The amount of secreted IgG analyzed by the system showed a weak negative linear correlation with the specific growth of isolated clones. An immunofluorescent microscopy study showed that the established clones could be used to analyze the intracellular secretion bottleneck. This is the first study to report the use of fluorescent protein fusion IgG as a tool to analyze the secretion of recombinant CHO cells. |
| تدمد: | 1573-0778 0920-9069 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4671af2675799d8f287a4c59bda9725bTest https://doi.org/10.1007/s10616-018-0276-7Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4671af2675799d8f287a4c59bda9725b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15730778 09209069 |
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