Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension
| العنوان: | Differential expression of miRNAs are associated with the insulin signaling pathway in preeclampsia and gestational hypertension |
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| المؤلفون: | Irene Mackraj, Jagidesa Moodley, Saravanakumar Murugesan, Olive P. Khaliq |
| المصدر: | Clinical and Experimental Hypertension. 40:744-751 |
| بيانات النشر: | Informa UK Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, 0301 basic medicine, Gestational hypertension, medicine.medical_specialty, Physiology, Placenta, Blood Pressure, 030204 cardiovascular system & hematology, Real-Time Polymerase Chain Reaction, Preeclampsia, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Pre-Eclampsia, Western blot, Pregnancy, Internal medicine, microRNA, Internal Medicine, medicine, Humans, Insulin, Differential expression, Protein kinase B, PI3K/AKT/mTOR pathway, medicine.diagnostic_test, business.industry, Hypertension, Pregnancy-Induced, General Medicine, medicine.disease, MicroRNAs, 030104 developmental biology, Endocrinology, Gestation, Female, Phosphatidylinositol 3-Kinase, business, Proto-Oncogene Proteins c-akt, Signal Transduction |
| الوصف: | The objective of this study was to determine microRNAs (miRNAs) expression levels in placental tissue and serum samples from preeclampsia (PE) and gestational hypertensive (GH) patients.Using a targeted qPCR method, the selected miRNAs putatively involved in the PE and GH were examined from normotensive (n = 32), PE (n = 32) and GH (n = 28) in South African women. Western blot analysis of protein expressions of AKT and PI3K was performed in the placental tissue of all three groups.qPCR results of serum miR-222 expression levels showed a significant decrease in PE compared to GH and normotensive groups. miR-29a expression levels were significantly increased in PE and GH groups compared to normotensives. Serum expression levels of miR-181a in GH showed a significant increase compared to the PE and normotensive groups. Placental tissue expression levels of miR-181a were significantly increased in PE and GH groups compared to normotensives. Western blot results of placental tissue showed a decrease in the expression levels of AKT-serine and threonine in the PE groups compared to the normotensives and a significantly higher expression in the GH groups compared to normotensives. Phosphatidyl-inositol-3 kinase (PI3K) expression levels were significantly decreased in PE and GH groups compared to normotensives.The present study, interestingly, demonstrates the differential expression of circulating miRNA in GH and a correlation between the expression levels of miRNAs with AKT/PI3K in the insulin signaling pathway, reinforcing the presence of metabolic dysregulation in PE and GH. |
| تدمد: | 1525-6006 1064-1963 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46405b5d53b00e9c1ae4854752819262Test https://doi.org/10.1080/10641963.2018.1431257Test |
| رقم الانضمام: | edsair.doi.dedup.....46405b5d53b00e9c1ae4854752819262 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15256006 10641963 |
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