نتائج البحث - "Ruminococcaceae"

1

METAGENOMICS IN-SIGHT INTO THE MICROBIAL STRUCTURAL DIVERSITY OF ANAEROBIC DIGESTER UTILISING FRUIT WASTE AS SUBSTRATE

المؤلفون: Akin-Osanaiye, Uwem Okon, Ogemdi Chinwendu, Edet, Anika, Bukola Catherine

المصدر: Journal of microbiology, biotechnology and food sciences. 9:965-969

الوصف: Anaerobic digester has emerged as a technology of choice in management of waste and production of biogas. However, the microbial ecology of digesters utilizing various substrates are very poorly understood. The ecology of anaerobic digester utilizing Citrullus lanatus fruit waste was analyzed using metagenomics. Slurry substrate sample was collected from a functional digester aseptically and anaerobically. Metagenomic DNA was extracted using ZYMO DNA extraction Kit (Model D 6001, Zymo Research, USA) following manufacturer’s instruction. Extracted DNA was amplified using the 16S rRNA gene amplicon PCR primers set and sequenced using Illumina MiSeq platform. Taxonomic analysis of the reads was performed using NCBI-BLAST-2.2.24 and CLC bio Genomics workbench v7.5.1. Taxonomic classification of the sequences revealed that bacteria and archae were the top two kingdoms with reads counts of 57,554 and 80, respectively. The top 7 phyla were Unknown, Firmicutes, Proteobacteria, Planctomycetes, Bacteroidetes, Actinobacteria and Euryarchaeota in decreasing order of counts. A total of 30 microbial classes, 43 orders, 79 families and 210 species were further classified. Over half of the 210 species detected were not routinely cultured species in the laboratory, indicating that there is still a relatively wide gap between culturable and non-culturable species in an anaerobic digester. The Acidogens captured in this study were Clostridium, Uncultured rumen bacteria and Bacteroidetes species. Furthermore, we also detected uncultured syntrophic Acetogens such as Syntrophomonas species and the hydrogenotrophic thermophile, Methanothermobacter sp. The Syntrophomonas species is known to breakdown short chain fatty acids, like propionate and butanoate in concert with hydrogenotrophic Methanogens indicating methane generation was via the hydrogenotrophic route. However, the main representative hydrogenotrophic methanogens detected were Methanoculleus bourgensis and Methanoculleus marisnigri, with the former being more abundant. In addition to the aforementioned species, other species captured were largely classified as unknown or uncultured species and they include Uncultured species of Clostridium, Syntrophomonas, Synergistetes, Synergistaceae Anaerobic sp, Ruminococcaceae, Rumen sp, Thermomonas, Thermoanaerobacteriales, Bacterium, Compost, Firmicutes, Bacteroidetes, Chloroflexi, Clostriaceae, Acetobacter. Eubacterium, Alpha and Aacteroides. The results of the study revealed that culture-independent approach is better able to capture the anaerobes including both culturable and unknown that dominate anaerobic digesters and are responsible for the bioconversion of organic waste into biogas.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::0381da6389fa5be7e67be26a8a703b1cTest
https://doi.org/10.15414/jmbfs.2020.9.5.965-969Test

2

Minor Changes in the Composition and Function of the Gut Microbiota During a 12-Week Whole Grain Wheat or Refined Wheat Intervention Correlate with Liver Fat in Overweight and Obese Adults

المؤلفون: Suzan Wopereis, Guido J. E. J. Hooiveld, Mara P H van Trijp, Lydia A. Afman, Diederik Esser, Sophie Schutte, Femke P M Hoevenaars

المصدر: The Journal of Nutrition
Hepatobiliary Surg Nutr
The Journal of Nutrition, 151(3), 491-502
The Journal of Nutrition 151 (2020) 3

مصطلحات موضوعية: Dietary Fiber, Male, 0301 basic medicine, Flour, Medicine (miscellaneous), Overweight, Gut flora, Voeding, Metabolisme en Genomica, Feces, 0302 clinical medicine, Nonalcoholic fatty liver disease, fermentation, Whole Grains, Nutrition and Dietetics, biology, Fatty liver, Middle Aged, Metabolism and Genomics, Health & Consumer Research, Liver, Metabolisme en Genomica, Nutrition, Metabolism and Genomics, Female, 030211 gastroenterology & hepatology, medicine.symptom, Metabolic Networks and Pathways, Ruminococcaceae, medicine.medical_specialty, refined wheat, Aspartate transaminase, AcademicSubjects/MED00060, 03 medical and health sciences, Voeding, Double-Blind Method, Internal medicine, medicine, Humans, human, Food, Health & Consumer Research, VLAG, Nutrition, Aged, fatty liver, gut microbiota, business.industry, Biochemical, Molecular, and Genetic Mechanisms, overweight/obesity, medicine.disease, biology.organism_classification, Obesity, Gastrointestinal Microbiome, Gastrointestinal Tract, 030104 developmental biology, Endocrinology, Alanine transaminase, Food, Commentary, biology.protein, AcademicSubjects/SCI00960, whole grain wheat, Edible Grain, business, Biomarkers

الوصف: Background Whole grain wheat (WGW) products are advocated as a healthy choice when compared with refined wheat (RW). One proposed mechanism for these health benefits is via the microbiota, because WGW contains multiple fibers. WGW consumption has been proposed to ameliorate nonalcoholic fatty liver disease, in which microbiota might play a role. Objectives We investigated the effect of WGW compared with RW intervention on the fecal microbiota composition and functionality, and correlated intervention-induced changes in bacteria with changes in liver health parameters in adults with overweight or obesity. Methods We used data of a 12-wk double-blind, randomized, controlled, parallel trial to examine the effects of a WGW (98 g/d) or RW (98 g/d) intervention on the secondary outcomes fecal microbiota composition, predicted microbiota functionality, and stool consistency in 37 women and men (aged 45–70 y, BMI 25–35 kg/m2). The changes in microbiota composition, measured using 16S ribosomal RNA gene sequencing, after a 12-wk intervention were analyzed with nonparametric tests, and correlated with changes in liver fat and circulating concentrations of liver enzymes including alanine transaminase, aspartate transaminase, γ-glutamyltransferase, and serum amyloid A. Results The WGW intervention increased the mean (± SD) relative abundances of Ruminococcaceae_UCG-014 (baseline: 2.2 ± 4.6%, differential change over time (Δ) 0.51 ± 4.2%), Ruminiclostridium_9 (baseline: 0.065 ± 0.11%, Δ 0.054 ± 0.14%), and Ruminococcaceae_NK4A214_group (baseline: 0.37 ± 0.56%, Δ 0.17 ± 0.83%), and also the predicted pathway acetyl-CoA fermentation to butyrate II (baseline: 0.23 ± 0.062%, Δ 0.035 ± 0.059%), compared with the RW intervention (P values

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38d7e93d9e4e2bef68a6ff4a87002fc2Test
https://doi.org/10.1093/jn/nxaa312Test

3

Host genetics and gut microbiota cooperatively contribute to azoxymethane-induced acute toxicity in Collaborative Cross mice

المؤلفون: Jian-Hua Mao, Fuling Zhou, Sun-Young Lee, Chenhan Zhong, Hang Chang, Li He, Antoine M. Snijders, Ying Yuan, David W. Threadgill, Yuqing Zhang, Yankai Xia, Susan E. Celniker

المصدر: Archives of Toxicology. 95:949-958

الوصف: Azoxymethane (AOM) is a widely used carcinogen to study chemical-induced colorectal carcinogenesis and is an agent for studying fulminant hepatic failure. The inter-strain susceptibility to acute toxicity by AOM has been reported, but its association with host genetics or gut microbiota remains largely unexplored. Here a cohort of genetically diverse Collaborative Cross (CC) mice was used to assess the contribution of host genetics and the gut microbiome to AOM-induced acute toxicity. We observed variation in AOM-induced acute liver failure across CC strains. Quantitative trait loci (QTL) analysis revealed three chromosome regions significantly associated with AOM toxicity. Genes located within these QTL, including peroxisome proliferator-activated receptor alpha (Ppara), were enriched for enzyme activator and nucleoside-triphosphatase regulator activity. We further demonstrated that the protein level of PPARα in liver tissues from sensitive strains was remarkably lower compared to levels in resistant strains, consistent with protective role of PPAR family in liver injury. We discovered that the abundance levels of gut microbial families Anaeroplasmataceae, Ruminococcaceae, Lactobacillaceae, Akkermansiaceae and Clostridiaceae were significantly higher in the sensitive strains compared to the resistant strains. Using a random forest classifier method, we determined that the relative abundance levels of these microbial families predicted AOM toxicity with the area under the receiver-operating curve (AUC) of 0.75. Combining the three genetic loci and five microbial families increased the predictive accuracy of AOM toxicity (AUC of 0.99). Moreover, we found that Ruminococcaceae and Lactobacillaceae acted as mediators between host genetics and AOM toxicity. In conclusion, this study shows that host genetics and specific microbiome members play a critical role in AOM-induced acute toxicity, which provides a framework for analysis of the health effects from environmental toxicants.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::78fbfa4f896d26a00e61392bcb007197Test
https://doi.org/10.1007/s00204-021-02972-xTest

4

Different Bifidobacterium bifidum strains change the intestinal flora composition of mice via different mechanisms to alleviate loperamide-induced constipation

المؤلفون: Jianxin Zhao, Hao Zhang, Wei Chen, Mao Chai, Xinping Li, Wang Gang, Linlin Wang

المصدر: Food & Function. 12:6058-6069

الوصف: Constipation is a condition with a high prevalence rate worldwide and may occur in men and women of any age. Bifidobacterium bifidum has been shown to have a relieving effect on constipation, but the underlying mechanism is still unknown. This study explored the effects of gavage of three strains of B. bifidum (CCFM668, FHNFQ25M12 and FXJCJ32M2) from different sources in mice with loperamide-induced constipation. After 38 days of intervention, B. bifidum CCFM668, FHNFQ25M12 and FXJCJ32M2 showed the ability to modify the levels of gastrointestinal active peptides and promote the expression of 5-hydroxytryptamine (5-HT or serotonin) receptor 4 (5-HT4R), thereby promoting small intestinal peristalsis. The strains could also effectively increase the thickness of the colonic mucosa. However, what was different from previous studies was that these results were independent of the levels of short-chain fatty acids (SCFAs) and 5-HT. Further analysis of the intestinal flora revealed that the relative abundances of the genera Faecalibaculum and Ruminococcaceae_UCG_014 in the constipated mice increased significantly, whereas that of Erysipelatoclostridium decreased. A correlation analysis between the intestinal flora and evaluated gastrointestinal indicators demonstrated that the relative abundances of the genera Anaerotruncus, Angelakisella, Erysipelatoclostridium and Ruminococcaceae_UCG_014 were negatively correlated with the levels of gastrointestinal active peptides. B. bifidum FXJCJ32M2 can increase the relative abundances of Turicibacter and Dubosiella, and this was positively correlated with the expression of aquaporin 8 and vasoactive intestinal peptide receptor 1 but could not effectively alleviate faecal dryness or promote colonic motility. These findings suggest that B. bifidum shows significant intraspecific differences in the remission mechanism and provides a theoretical basis for subsequent population experiments and personalised treatment for constipation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::26c6466affe5632e6b4454b16eda22bdTest
https://doi.org/10.1039/d1fo00559fTest

5

Deciphering diet-gut microbiota-host interplay: Investigations of pectin

المؤلفون: Shaoping Nie, Huizi Tan

المصدر: Trends in Food Science & Technology. 106:171-181

الوصف: Background Pectin exists in a broad range of plants, and acts as functional food additives with modulatory effects on colonic inflammation and metabolism dysfunctions, which underscores its promising prospects in commercialization. Moreover, food ingredients like pectin can impact the gut microbiota, and the degradation capabilities of these commensals may in turn determine the efficacy of dietary intervention. Scope and approach In this review, the pectin-host axis was figured by summarizing the physiological functions of pectin and the associated mechanisms; The pectin-gut microbiota axis was described via discussing the impact of pectin on intestinal microbiota composition; And the gut microbiota-host axis was discussed through describing the beneficial functions of pectinolytic commensals and their metabolites. Key findings and conclusions The structure properties of pectin including degree of esterification and branched chains played critical roles in their physiological functions of metabolism regulation, inflammation modulation, and carcinogen prevention. Meanwhile, distinct diversity, enterotypes or composition of gut microbiota can be developed under interventions of pectin with various structure characteristics, and pectin-induced dominant bacteria like Ruminococcaceae, Succinivibrionaceae, Lachnospiraceae and Bacteroidaceae and their metabolites exhibited relevant health-promoting functions. All these data are fundamental to establish the precise networks and further discover underlying mechanisms of pectin-gut microbiota-host interactions and can optimize the development of pectin in food and health care products.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::3e659f38dd92a0e7dbdadfee31b562a6Test
https://doi.org/10.1016/j.tifs.2020.10.010Test

6

Feed additives for the control of post-weaning Streptococcus suis disease and the effect on the faecal and nasal microbiota

المؤلفون: Sebastian Napp, Laia Sobrevia, Virginia Aragon, Lourdes Migura-Garcia, Carlos Neila-Ibáñez, Sergio López-Soria, Simon Tibble, Florencia Correa-Fiz, Blanca Martinez

المساهمون: Producció Animal, Sanitat Animal

المصدر: Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
Scientific Reports
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
IRTA Pubpro. Open Digital Archive
Institut de Recerca i Tecnologia Agroalimentàries (IRTA)

مصطلحات موضوعية: 0301 basic medicine, Streptococcus suis, Swine, Polymerase Chain Reaction, Feces, chemistry.chemical_compound, fluids and secretions, Anti-Infective Agents, Pregnancy, 2. Zero hunger, Multidisciplinary, Antimicrobials, Microbiota, Fatty Acids, Agriculture, Antimicrobial, 3. Good health, Parity, Treatment Outcome, Medicine, Female, Lysozyme, medicine.drug, Ruminococcaceae, DNA, Bacterial, Animal feed, Offspring, Science, 030106 microbiology, Microbial communities, Weaning, Biology, Article, Microbiology, 03 medical and health sciences, Streptococcal Infections, medicine, Animals, Amoxicillin, biology.organism_classification, Animal Feed, Nasal Mucosa, 030104 developmental biology, chemistry, Dietary Supplements, Food Additives, Muramidase

الوصف: Medicated feed is a common strategy to control the occurrence of Streptococcus suis disease in swine production, but feed additives may constitute an alternative to metaphylaxis. In a farm with post-weaning S. suis disease, the following additives were tested: lysozyme (Lys), medium chain fatty acids plus lysozyme (FA + Lys), FA plus a natural anti-inflammatory (FA + antiinf) and amoxicillin (Amox). During the course of the study, FA + antiinf and Amox groups showed lower prevalence of clinical signs compatible with S. suis disease than the rest of the groups. Piglets from the FA + antiinf group showed high diversity and richness in their nasal and faecal microbiota. Diet supplements did not have major effects on the faecal microbiota, where the genus Mitsuokella was the only differentially present in the FA + Lys group. In the nasal microbiota, piglets from FA + antiinf presented higher differential abundance of a sequence variant from Ruminococcaceae and lower abundance of an unclassified genus from Weeksellaceae. In general, we detected more significant changes in the nasal than in the feacal microbiota, and found that parity of the dams affected the microbiota composition of their offspring, with piglets born to gilts exhibiting lower richness and diversity. Our results suggest that additives could be useful to control post-weaning disease when removing antimicrobials in farms.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9adce5eee9c11c504091b88b4b49642bTest
https://doaj.org/article/2ad2ad94d62743639a465a285bfe0612Test

7

The characteristics of intestinal flora of IBS‐D with different syndromes

المؤلفون: Guanqun Chao, Shuo Zhang

المصدر: Immunity, Inflammation and Disease, Vol 8, Iss 4, Pp 615-628 (2020)
Immunity, Inflammation and Disease

مصطلحات موضوعية: Adult, Diarrhea, Male, lcsh:Immunologic diseases. Allergy, 0301 basic medicine, Enterobacteriales, Immunology, Physiology, Veillonellaceae, spleen–kidney‐yang deficiency, Biology, medicine.disease_cause, Irritable Bowel Syndrome, Feces, 03 medical and health sciences, 0302 clinical medicine, RNA, Ribosomal, 16S, medicine, Humans, Immunology and Allergy, Irritable bowel syndrome, Original Research, diarrhea‐predominant irritable bowel syndrome, liver‐depression and spleen‐deficiency, Streptococcus, Fusobacteria, Middle Aged, biology.organism_classification, medicine.disease, Enterobacteriaceae, Gastrointestinal Microbiome, Bifidobacteriaceae, 030104 developmental biology, Female, lcsh:RC581-607, intestinal flora, 030215 immunology, Ruminococcaceae

الوصف: Objective To study the distribution of fecal microbiota in diarrhea‐predominant irritable bowel syndrome (IBS‐D) patients of spleen–kidney‐yang deficiency and liver depression and spleen deficiency, to make an objective foundation for dialectics of different type of IBS‐D. And to provide the clinical doctors an experimental basis for medication by regulating dysbacteriosis. Methods We collected feces from the control group, spleen–kidney‐yang deficiency IBS‐D group, and liver‐depression and spleen‐deficiency IBS‐D group. After the extraction of fecal DNA, global DNA was isolated from every sample, and 16S ribosomal RNA was sequenced, and then we analyzed the results for bacteria such as Alpha diversity, community composition, LEfSe, and partial least squares discriminant analysis. Results We compared the changes among the fecal bacteria in the intestine of the IBS‐D patients and healthy controls and found the specificity of spleen–kidney‐yang deficiency syndrome and liver‐depression and spleen‐deficiency syndrome. The control group has the highest flora diversity (control group > liver‐depression and spleen‐deficiency > spleen–kidney‐yang deficiency group). The control group, spleen–kidney‐yang deficiency group, and liver‐depression and spleen‐deficiency group are different in phylum (Actinobacteria, Fusobacteria), class (Actinobacteria, Fusobacteria), order (Enterobacteriales, Bifidobacteriales, Fusobacteriales), and family (Bifidobacteriaceae, Ruminococcaceae, Enterobacteriaceae, Acidaminococcaceae, Veillonellaceae, Fusobacteriaceae). Bifidobacteriaceae and Ruminococcaceae in the control group, Enterobacteriales, Fusobacteriales, Acidaminococcaceae, and Phascolarctobacterium in the spleen–kidney‐yang deficiency group, and streptococcus are the specific bacteria in the liver‐depression and spleen‐deficiency group. Intestinal flora disturbance is closely related to IBS‐D. Conclusions There is a correlation between traditional Chinese medicine syndrome type and intestinal flora. The control group, the spleen–kidney‐yang deficiency group, and the liver‐depression and spleen‐deficiency group have specific bacteria.
1.Irritable bowel syndrome (IBS) is one of the most frequent gastrointestinal disorders. Transforming growth factor‐beta 1 (TGF‐β1) is in the colonic tissue and serum of IBS rats. 2.It is suspected in our study that increased chronic renal failure and interleukin 1 beta (IL‐1β) are involved in the mechanism of IBS. 3.Further research will be done to detect the interacting and inter adjusting mechanism of IL‐1β and other cytokines in IBS patients, and further study should be done to explore the role of TGF‐β1.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::951e7ef6275111dae142ea09f773630cTest
https://doi.org/10.1002/iid3.348Test

8

Effects of Agaricus blazei Murrill polysaccharides on hyperlipidemic rats by regulation of intestinal microflora

المؤلفون: Peige Du, Guangyu Xu, Yuxin Li, Han Xiao, Yingna Li, Xiao Li, Liping An, Yu Sheng, Xuechun Lu, Guo Xiao

المصدر: Food Science & Nutrition, 8(6):2758-2772
Food Science & Nutrition, Vol 8, Iss 6, Pp 2758-2772 (2020)

الوصف: The present research envisaged the effects of Agaricus blazei Murrill polysaccharides (ABPs) on blood lipids and its role in regulation of the intestinal microflora in hyperlipidemic rats. The acidic polysaccharide fraction of Agaricus blazei Murrill was obtained by DEAE-cellulose ion exchange column chromatography. The sugar content of ABP was 75.1%. Compared with the model group (MG), the serum TC, TG, and LDL-C levels decreased (p < .05 or p < .01) and the HDL-C levels increased (p < .01) significantly in the ABP group. Expression of CYP7A1 was up-regulated (p < .01), and that of SREBP-1C (p < .05) was down-regulated significantly in the liver tissue of rats in the ABP group. Additionally, the disordered hepatic lobules and the steatosis of hepatocytes were found to be significantly alleviated in the ABP group. We believe that ABP can reduce the ratio of Firmicutes/Bacteroidetes and reduce the relative abundance of Firmicutes, Ruminococcaceae_unclassified, and Ruminococcaceae, increasing the relative abundance of Proteobacteria, Clostridium_sensu_stricto, Allobaculum, Peptostreptococcaceae, Clostridiaceae_1, and Erysipelotrichaceae as targets to regulate blood lipids. The results showed ABP could regulate the dyslipidemia in rats with hyperlipidemia. The mechanism may be through the regulation of the imbalance of intestinal microflora induced by the high-fat diet in rats, which may be one of the important ways of its intervention on the dyslipidemia induced by high-fat diet.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8819e2cb3771bcdb4f4fbdcecf6f18a2Test
https://doi.org/10.1002/fsn3.1568Test

9

Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome

المؤلفون: E. I. Ermolenko, I. V. Kudryavtsev, O. I. Solovyova, A. B. Karaseva, A. N. Tsapieva, Yu. D. Kondratenko, M. P. Kotyleva, M. K. Serebryakova, I. A. Shumikhina, A. N. Suvorov

المصدر: Medicinskaâ Immunologiâ, Vol 22, Iss 2, Pp 335-346 (2020)

الوصف: The following specificcharacteristics of the composition of intestinal microbiota in patients with irritable bowel syndrome (IBS) were identified using a metagenomic analysis (16 S rRNA): 1) an increase in the representation of Actinobacteria, including Bifidobacterium spp., Firmicutes, including representatives of Streptococcaceae (Streptococcus), Lachnosperaceae (Dorea), Veillonellaceae (Dialister), Proteobacteria (Enterobacteriaceae and Desulfovibrionaceae families); 2) a decrease in the population of Bacteroidetes, including representatives of the families Prevotellacea (Prevotella spp.), Bacteroidaceae (Bacteroides spp.). Firmicutes belonging to the families Clostridiaceae and Ruminococcaceae (Fecalibacterium spp.). Flow cytometry in the study of the subpopulation composition of T regulatory (Treg) lymphocytes in patients with IBS revealed an increase in the number of CD45R0+CD62L+ central memory cells (CM), which can regulate the processes of maturation and differentiation of lymphocytes in lymphoid tissue. A decrease in the expression of exonucleases CD39 and CD73 was detected, which can have a significant effect on their activity. A reduction in effector memory cells (EM) Treg was observed. Changes in the expression level of exonucleases CD39 and CD73 were inversely correlated with the content of Proteobacteria and the representation of the genera Bifidobacterium spp. and Faecalibacterium spp. The content of СЫ Treg was directly correlated with the content of Dorea spp. The results may be indicative of impairment in the processes of Treg differentiation, which are closely related to changes in key components of intestinal microbiocenosis in IBS.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a77b26bd32fc82621a3fedf86f71c17Test
https://www.mimmun.ru/mimmun/article/view/1905Test

10

Hydrogeniiclostidium mannosilyticum gen. nov., sp. nov. isolated from human faeces

المؤلفون: Natalia E. Suzina, Dmitriy Yurievich Trofimov, Sofia Romanovna Sokolova, Boris A. Efimov, Andrei V. Chaplin, Victoria A. Shcherbakova, Andrey Yurievich Goltsov, Taisiya Olegovna Kochetkova

المصدر: International Journal of Systematic and Evolutionary Microbiology. 70:1210-1216

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Whole genome sequencing, Phylogenetic tree, biology, Strain (chemistry), General Medicine, biology.organism_classification, 16S ribosomal RNA, 010603 evolutionary biology, 01 natural sciences, Microbiology, Clostridia, 03 medical and health sciences, genomic DNA, 030104 developmental biology, Genus, Ecology, Evolution, Behavior and Systematics, Ruminococcaceae

الوصف: A strain of obligately anaerobic, spore-forming, Gram-positive rods was isolated from child faeces and characterized both phenotypically and genotypically. Phylogenetic analysis based on 16S rRNA gene and whole genome sequencing revealed the strain to represent a member of the family Ruminococcaceae distant from described species and genera. The strain was moderately saccharolytic with mannose as the preferred substrate and produced lactic acid, acetic acid and H2 as the end products. The major cellular long-chain fatty acids were C16 : 0 and C16 : 0 aldehyde. The genomic DNA G+C content was 52.3 mol%. On the basis of chemotaxonomic and genomic properties it was concluded that the strain represents a novel species in a new genus within the family Ruminococcaceae , for which the name Hydrogeniiclostidium mannosilyticum gen. nov., sp. nov. is proposed. The type strain of Hydrogeniiclostidium mannosilyticum is ASD2818T (=VKM B-3268T=JCM 33295T).

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::085c84f87d08c48e691a11f8b0d07b19Test
https://doi.org/10.1099/ijsem.0.003900Test

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