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61

Association betweenMMP3andTIMP3polymorphisms and risk of osteoarthritis

المؤلفون: Liang Yan, Dingjun Hao, Zhichao Tong, Bo Chen, Yang Liu

المصدر: Oncotarget

الوصف: // Zhichao Tong 1, * , Yang Liu 2, * , Bo Chen 1 , Liang Yan 3 and Dingjun Hao 3 1 Department of Bone Diesase and Bone Tumor, Hong Hui Hospital, Xi’an Jiaotong University College of Medicine, Shaanxi 710054, China 2 Department of Orthopaedics, Hong Hui Hospital, Xi’an Jiaotong University College of Medicine, Shaanxi 710054, China 3 Department of Spinal Surgery, Hong Hui Hospital, Xi’an Jiaotong University College of Medicine, Shaanxi 710054, China * These authors have contributed equally to this work and should be considered as co-first authors Correspondence to: Liang Yan, email: liangyanhonghui@163.com Dingjun Hao, email: dingjunhaohonghui@163.com Keywords: MMP-3, TIPM-3, osteoarthritis, polymorphisms Received: December 21, 2016 Accepted: March 08, 2017 Published: June 27, 2017 ABSTRACT Osteoarthritis (OA) is the most commonly occurring degenerative joint disease worldwide, and its incidence has increased in recent years. We evaluated whether there is the association between MMP-3 and TIMP-3 variants and susceptibility to OA in a Chinese population. Venous blood samples were collected from 431 female participants (200 cases and 231 controls) at Hong Hui Hospital, Xi’an Jiaotong University College of Medicine between 2015 and 2016. After genotyping the samples using standard protocols, the association between MMP-3 and TIMP-3 single nucleotide polymorphisms and risk of OA was assessed by calculating odds ratios (ORs) and 95% confidence intervals (95% CIs) using unconditional logistic regression analysis. The minor G allele of rs650108 was associated with OA risk in a recessive model ( p = 0.034, OR = 1.82, 95%CI = 1.04-3.18), while the minor A allele of rs715572 was associated with OA risk in a recessive model ( p = 0.030, OR = 1.88, 95%CI = 1.05-3.34). Thus a suggestive association was observed in a discovery case-control study between OA and two common SNPs, rs650108 in MMP-3 and rs715572 in TIMP-3 .

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01ada09dbf8c4549ada2cf4eb3195d1bTest
https://doi.org/10.18632/oncotarget.18745Test

62

Alterations of Histone Modifications Contribute to Pregnane X Receptor-Mediated Induction of CYP3A4 by Rifampicin

المؤلفون: Liang Yan, Yiting Wang, Jingyang Liu, Quancheng Kan, Yali Nie, Xiao-bo Zhong, Lirong Zhang

المصدر: Molecular Pharmacology. 92:113-123

الوصف: CYP3A4 is one of the major drug-metabolizing enzymes in human and is responsible for the metabolism of 60% of clinically used drugs. Many drugs are able to induce the expression of CYP3A4, which usually causes drug-drug interactions and adverse drug reactions. This study aims to explore the role of histone modifications in rifampicin-induced expression of CYP3A4 in LS174T cells. We found that the induction of CYP3A4 mRNA (4- to 15-fold) by rifampicin in LS174T cells was associated with increased levels of histone H3 lysine 4 trimethylation (H3K4me3, above 1.8-fold) and H3 acetylation (above 2-fold) and a decreased level of histone H3 lysine 27 trimethylation (H3K27me3, about 50%) in the CYP3A4 promoter. Rifampicin enhanced recruitment to the CYP3A4 promoter of nuclear receptor coactivator 6 (NCOA6, above 3-fold) and histone acetyltransferase p300 (p300, above 1.6-fold). Silencing NCOA6 or p300 by short-hairpin RNAs resulted in inhibition of the CYP3A4 induction as well as altered levels of H3K4me3, H3K27me3, or H3 acetylation in the CYP3A4 promoter. Knockdown of pregnane X receptor (PXR) expression not only suppressed the recruitment of NCOA6 and p300 but also abolished the changes caused by rifampicin in H3K4me3, H3K27me3, and H3 acetylation levels in the CYP3A4 promoter. Moreover, rifampicin treatment enhanced the nuclear accumulation and interactions between PXR and NCOA6/p300. In conclusion, we show that the alterations of histone modifications contribute to the PXR-mediated induction of CYP3A4 by rifampicin.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d806edd2c6fd0b7e18a1e6e674628845Test
https://doi.org/10.1124/mol.117.108225Test

63

The association between MTHFR gene C677T polymorphism and ALL risk based on a meta-analysis involving 17,469 subjects

المؤلفون: Bei-Bei Zhang, Weiming Zhang, Daogang Wang, Liang Yan

المصدر: Clinica Chimica Acta. 466:85-92

الوصف: Background The methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism is closely related to the acute lymphoblastic leukaemia (ALL) indicated by many previous epidemiologic studies. However, their conclusions were still conflicting. Methods Our aim is to evaluate their associations using a more comprehensive updated meta-analysis. Electronic searches were conducted to select published studies prior to February, 2016. Results Totally, 39 case-control studies including 6551 ALL cases and 10,918 controls were selected in current meta-analysis. The association was detected significantly between MTHFR C677T polymorphism and ALL reducing susceptibility. Conclusions Our results indicate that the MTHFR C677T polymorphism may be a promising ALL biomarker and studies to explore the protein levels of the variants and their functional role are required for the definitive conclusions.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc28af7199d4a78989796b1955f45eeeTest
https://doi.org/10.1016/j.cca.2017.01.001Test

64

Molecular Scree ning of Blast Resistance Genes in Rice Germplasms Resistant to Magnaporthe oryzae

المؤلفون: Wu Han-Lin, Yan Bai-Yuan, Zhi-juan Ji, Zeng Yu-xiang, Yang Changdeng, Peng Yun-Liang, Liang Yan

المصدر: Rice Science, Vol 24, Iss 1, Pp 41-47 (2017)

الوصف: Molecular screening of major rice blast resistance genes was determined with molecular markers, which showed close-set linkage to 11 major rice blast resistance genes (Pi-d2, Pi-z, Piz-t, Pi-9, Pi-36, Pi-37, Pi5, Pi-b, Pik-p, Pik-h and Pi-ta2), in a collection of 32 accessions resistant to Magnaporthe oryzae. Out of the 32 accessions, the Pi-d2 and Pi-z appeared to be omnipresent and gave positive express. As the second dominant, Pi-b and Piz-t gene frequencies were 96.9% and 87.5%. And Pik-h and Pik-p gene frequencies were 43.8% and 28.1%, respectively. The molecular marker linkage to Pi-ta2 produced positive bands in eleven accessions, while the molecular marker linkage to Pi-36 and Pi-37 in only three and four accessions, respectively. The natural field evaluation analysis showed that 30 of the 32 accessions were resistant, one was moderately resistant and one was susceptible. Infection types were negatively correlated with the genotype scores of Pi-9, Pi5, Pi-b, Pi-ta2 and Pik-p, although the correlation coefficients were very little. These results are useful in identification and incorporation of functional resistance genes from these germplasms into elite cultivars through marker-assisted selection for improved blast resistance in China and worldwide.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f7ce68ae1ba21d0e47714555508f9bfTest
https://doi.org/10.1016/j.rsci.2016.07.004Test

65

Berberine augments ATP-induced inflammasome activation in macrophages by enhancing AMPK signaling

المؤلفون: Liang Yan, Hong-Xia Wei, Chen-Guang Li, Yan-Yun Jing, Hao Pan, Li-Hui Xu, Yi-Dan Liang, Dong-Yun Ouyang, Bo Hu, Qing-Bing Zha, Xian-Hui He

المصدر: Oncotarget

مصطلحات موضوعية: 0301 basic medicine, antibacterial infection, Berberine, Inflammasomes, MAP Kinase Signaling System, Neutrophils, Pharmacology, Mice, 03 medical and health sciences, chemistry.chemical_compound, Adenosine Triphosphate, Immune system, inflammasome, In vivo, AMP-activated protein kinase (AMPK), medicine, Animals, Immune response, Protein kinase A, Gene knockdown, Microbial Viability, business.industry, Macrophages, Research Paper: Immunology, Immunity, Pyroptosis, AMPK, Inflammasome, Bacterial Infections, 030104 developmental biology, Neutrophil Infiltration, Oncology, chemistry, Immunology, Macrophages, Peritoneal, Immunology and Microbiology Section, Female, business, medicine.drug

الوصف: // Chen-Guang Li 1,* , Liang Yan 1,* , Yan-Yun Jing 1,* , Li-Hui Xu 2 , Yi-Dan Liang 1 , Hong-Xia Wei 1 , Bo Hu 3 , Hao Pan 1 , Qing-Bing Zha 4 , Dong-Yun Ouyang 1 and Xian-Hui He 1 1 Department of Immunobiology, College of Life Science and Technology, Jinan University, Guangzhou, China 2 Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China 3 Department of Nephrology, the First Affiliated Hospital of Jinan University, Guangzhou, China 4 Department of Fetal Medicine, the First Affiliated Hospital of Jinan University, Guangzhou, China * These authors have contributed equally to this work Correspondence to: Xian-Hui He, email: // Keywords : antibacterial infection, berberine, AMP-activated protein kinase (AMPK), inflammasome, macrophages, Immunology and Microbiology Section, Immune response, Immunity Received : August 19, 2016 Accepted : December 01, 2016 Published : December 12, 2016 Abstract The isoquinoline alkaloid berberine possesses many pharmacological activities including antibacterial infection. Although the direct bactericidal effect of berberine has been documented, its influence on the antibacterial functions of macrophages is largely unknown. As inflammasome activation in macrophages is important for the defense against bacterial infection, we aimed to investigate the influence of berberine on inflammasome activation in murine macrophages. Our results showed that berberine significantly increased ATP-induced inflammasome activation as reflected by enhanced pyroptosis as well as increased release of caspase-1p10 and mature interleukin-1β (IL-1β) in macrophages. Such effects of berberine could be suppressed by AMP-activated protein kinase (AMPK) inhibitor compound C or by knockdown of AMPKα expression, indicating the involvement of AMPK signaling in this process. In line with increased IL-1β release, the ability of macrophages to kill engulfed bacteria was also intensified by berberine. This was corroborated by the in vivo finding that the peritoneal live bacterial load was decreased by berberine treatment. Moreover, berberine administration significantly improved survival of bacterial infected mice, concomitant with increased IL-1β levels and elevated neutrophil recruitment in the peritoneal cavity. Collectively, these data suggested that berberine could enhance bacterial killing by augmenting inflammasome activation in macrophages through AMPK signaling.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8d2e29a8ca670b49c4650c44d39de9eTest
https://doi.org/10.18632/oncotarget.13921Test

66

Biological function and mechanism of miR-33a in prostate cancer survival and metastasis: via downregulating Engrailed-2

المؤلفون: Liang Yan, Qi Li, Siyu Lu, Weidong Zhang, Li Xuyan, B. Qiao, G. Hou

المصدر: Clinical and Translational Oncology. 19:562-570

مصطلحات موضوعية: Male, 0301 basic medicine, Oncology, Cancer Research, medicine.medical_specialty, Blotting, Western, Down-Regulation, Mice, Nude, Apoptosis, Nerve Tissue Proteins, Real-Time Polymerase Chain Reaction, medicine.disease_cause, Metastasis, Mice, 03 medical and health sciences, Prostate cancer, 0302 clinical medicine, DU145, Cell Movement, Prostate, Cell Line, Tumor, Internal medicine, In Situ Nick-End Labeling, medicine, Animals, Humans, Transcription factor, Cell Proliferation, Homeodomain Proteins, Oncogene, business.industry, Prostatic Neoplasms, General Medicine, medicine.disease, Gene Expression Regulation, Neoplastic, Mice, Inbred C57BL, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, Heterografts, business, Carcinogenesis

الوصف: Recent studies have identified Engrailed-2 (EN-2), a homeobox-containing transcription factor, as a candidate oncogene in prostate cancer (PC). Therapeutic targeting on EN-2, however, is limited because the mechanism underlying EN-2 overexpression in prostatic cancer cells is unknown. This study was to investigate the potential regulatory role of miR-33a on EN-2 expression and explore this signaling axis in ability of prostate cancer survival and metastasis. The relative expression of miR-33a and EN-2 in paired prostate cancer tissue and adjacent normal tissue as well as in prostate cancer cell lines, PC3 and DU145, was determined using quantitative real-time PCR or western blot, respectively. Cells survival, migration and invasion were evaluated by assays of MTT, TUNEL and Boyden chamber assays, respectively. Direct regulation of EN-2 by miR-33a was examined by luciferase reporter assay. The data showed that miR-33a was upregulated and EN-2 was downregulated in both prostate cancer tissue and prostate cancer cells. miR-33a overexpression suppresses prostate cancer cell survival and metastasis. miR-33a can directly act on EN-2 expression by binding to 3′UTR of its mRNA. Also, miR-33a negatively regulated EN-2 mRNA and protein expression. In pcDNA-EN-2 and miR-33a mimic co-transfected PC3 and DU145 cells, EN-2 overexpression reverses the anti-cell survival and metastasis actions of miR-33a overexpression. The pivotal role of miR-33a in inhibiting prostate tumor growth was confirmed in xenograft models of prostate cancer. Our data suggest that the functional interaction of miR-33a and EN-2 is involved in tumorigenesis of prostate cancer. Also in this process EN-2 serves as a negative responder for miR-33a.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fee15fd19e986db8b7546181efcf37dTest
https://doi.org/10.1007/s12094-016-1564-3Test

67

Hepatic expression of transcription factors affecting developmental regulation of UGT1A1 in the Han Chinese population

المؤلفون: Lirong Zhang, Quan-cheng Kan, Shu-jie Wang, Pei Wang, Hong-zheng Bi, Xiang-Guang Meng, Jiang-feng Li, Yali Nie, Liang Yan, Hang He

المصدر: European Journal of Clinical Pharmacology. 73:29-37

الوصف: Complete or partial inactivity of UGT1A1, the unique enzyme responsible for bilirubin glucuronidation, is commonly associated with hyperbilirubinemia. We investigated the dynamic expression of UGT1A1, and that of the transcription factors (TFs) involved in its developmental regulation, during human hepatic growth in Han Chinese individuals.Eighty-eight prenatal, pediatric, and adult liver samples were obtained from Han Chinese individuals. Quantitative real-time polymerase chain reaction was used to evaluate mRNA expression of UGT1A1 and TFs including PXR, CAR, HNF1A, HNF4A, PPARA, etc. UGT1A1 protein levels and metabolic activity were determined by western blotting and high-performance liquid chromatography. Direct sequencing was employed to genotype UGT1A1*6 (211G˃A) and UGT1A1*28 (TA6˃TA7) polymorphisms.UGT1A1 expression was minimal in prenatal samples, but significantly elevated during pediatric and adult stages. mRNA and protein levels and metabolic activity were prominently increased (120-, 20-, and 10-fold, respectively) in pediatric and adult livers compared to prenatal samples. Furthermore, expression did not differ appreciably between pediatric and adult periods. Dynamic expression of TFs, including PXR, CAR, HNF1A, HNF4A, and PPARA, was consistent with UGT1A1 levels at each developmental stage. A pronounced correlation between expression of these TFs and that of UGT1A1 (P 0.001) was observed. Moreover, UGT1A1*6 and UGT1A1*28 polymorphisms reduced levels of UGT1A1 by up to 40-60 %.Hepatic expression of transcription factors is associated with developmental regulation of UGT1A1 in the Han Chinese population. Moreover, UGT1A1 polymorphisms are associated with reduced expression of UGT1A1 mRNA and protein, as well as enzyme activity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73b13e16e09c6d3b8671c9ac8963aeb0Test
https://doi.org/10.1007/s00228-016-2137-7Test

68

The contribution of indigenous non-Saccharomyces wine yeast to improved aromatic quality of Cabernet Sauvignon wines by spontaneous fermentation

المؤلفون: Lin Lu, Chang-Qing Duan, Guo-Liang Yan, Pei-Tong Liu

المصدر: LWT - Food Science and Technology. 71:356-363

الوصف: In this study, the aromatic profiles of Cabernet Sauvignon wine and the diversity of non-Saccharomyces yeast in industrial-scale spontaneous fermentation (SF) and inoculated fermentation (IF) were investigated. The wine obtained from SF had distinct aromatic profiles compared with IF wine. SF wine was characterized by a higher amount of medium-chain fatty acids, esters, terpenes (trans-rose oxide) and C13-norisoprenoids (β-damascenone), whereas IF wine featured more aldehydes and higher alcohols. Five non-Saccharomyces yeast species, including Hanseniaspora uvarum, Candida stella, Pichia fermentans, Issatchenkia orientalis and Metschnikowia pulcherrima, were isolated from SF, and their enological traits were examined in mixed fermentation with Saccharomyces cerevisiae, respectively. The mixed culture of P. fermentans and C. stella with S. cerevisiae could generate higher levels of esters, fatty acids and norisoprenoids compared to the monoculture of S. cerevisiae, and a co-culture of M. pulcherrima, I. orientalis and H. uvarum could accumulate higher amounts of higher alcohols. Our results suggested that non-Saccharomyces yeast strains greatly contributed to the fruity aroma quality of SF wine, and the mixed culture of Saccharomyces yeasts with non-Saccharomyces yeasts such as P. fermentans, C. stella or M. pulcherrima is a valuable tool to modulate the volatile profiles and improve the aromatic complexity of wine.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::b3c749db968f7f90351a9c3b7783d0f8Test
https://doi.org/10.1016/j.lwt.2016.04.031Test

69

MiR-19b suppresses PTPRG to promote breast tumorigenesis

المؤلفون: Chen-Yu Zhang, Chao Ye, Rong Yang, Xi Chen, Chihao Zhao, Yuanyuan Gu, Shufang Cui, Hongwei Liang, Yeting Hong, Liang Yan, Xin Yan, Minghui Liu, Yanqing Liu, Uzair Urrehman

المصدر: Oncotarget

مصطلحات موضوعية: 0301 basic medicine, Tumor suppressor gene, Cell Survival, proliferation, Breast Neoplasms, migration, medicine.disease_cause, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Cell Movement, Cell Line, Tumor, microRNA, Humans, Medicine, University medical, RNA, Messenger, RNA, Small Interfering, 3' Untranslated Regions, Cell Proliferation, Binding Sites, miR-19b, PTPRG, Receptor-Like Protein Tyrosine Phosphatases, Class 5, business.industry, Three prime untranslated region, apoptosis, Cancer, medicine.disease, Gene Expression Regulation, Neoplastic, MicroRNAs, Cell Transformation, Neoplastic, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, Pharmaceutical biotechnology, MCF-7 Cells, Cancer research, Female, business, Carcinogenesis, Research Paper

الوصف: // Minghui Liu 1, * , Rong Yang 1, 2, * , Uzair Urrehman 1, * , Chao Ye 1 , Xin Yan 3 , Shufang Cui 1 , Yeting Hong 1 , Yuanyuan Gu 1 , Yanqing Liu 1 , Chihao Zhao 1 , Liang Yan 4 , Chen-Yu Zhang 1 , Hongwei Liang 1 , Xi Chen 1 1 State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210046, China 2 Department of Urology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, China 3 Department of Respiratory Medicine, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, China 4 Provincial Key Laboratory of Biological Macro-molecules Research, Wannan Medical College, Wuhu, Anhui 241002, China * These authors contributed equally to this work Correspondence to: Xi Chen, email: xichen@nju.edu.cn Hongwei Liang, email: lianghongwei0418@163.com Chen-Yu Zhang, email: cyzhang@nju.edu.cn Keywords: miR-19b, PTPRG, proliferation, apoptosis, migration Received: January 05, 2016 Accepted: August 22, 2016 Published: September 01, 2016 ABSTRACT Protein tyrosine phosphatase receptor type G (PTPRG) is an important tumor suppressor gene in multiple human cancers. In this study, we found that PTPRG protein levels were downregulated in breast cancer tissues while the mRNA levels varied irregularly, implying a post-transcriptional mechanism was involved. Because microRNAs are powerful post-transcriptional regulators of gene expression, we used bioinformatics analysis to search for microRNAs that potentially targets PTPRG in the setting of breast cancer. We identified two specific binding sites for miR-19b in the 3′-untranslated region of PTPRG. We further identified an inverse correlation between miR-19b and PTPRG protein levels, but not mRNA levels, in human breast cancer tissues. By overexpressing or knocking down miR-19b in MCF-7 cells and MDA-231 cells, we experimentally confirmed that miR-19b directly suppresses PTPRG expression. Furthermore, we determined that the inhibition of PTPRG by miR-19b leads to increased proliferation, stimulated cell migration and reduced apoptosis. Taken together, our findings provide the first evidence that miR-19b inhibits PTPRG expression to promote tumorigenesis in human breast cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d749fa95726fa9ff9865f181b66e7165Test
https://doi.org/10.18632/oncotarget.11799Test

70

Crystal structure of (3aR,4R,5R,7R,8S,9R,9aS,12R)-7-ethyl-5-(1-hydroxy-2-((R)-3-hydroxypyrrolidin-1-yl)ethoxy)-4,7,9,12-tetramethyldecahydro-4,9a-propanocyclopenta[8]annulene-3,8-diol – a pleuromutilin derivative, C26H41NO5

المؤلفون: Liang Yan, Fu Yun-Xing, Liang Jian-Ping

المصدر: Zeitschrift für Kristallographie - New Crystal Structures. 233:923-925

مصطلحات موضوعية: 0301 basic medicine, Chemistry, Diol, Crystal structure, Annulene, Condensed Matter Physics, 030226 pharmacology & pharmacy, Medicinal chemistry, Inorganic Chemistry, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, 0302 clinical medicine, Alkoxy group, General Materials Science, Pleuromutilin, Derivative (chemistry)

الوصف: C26H41NO5, orthorhombic, P212121 (no. 19), a = 9.6178(8) Å, b = 13.3335(11) Å, c = 19.0758(16) Å, V = 2446.3(4) Å3, Z = 4, R gt(F) = 0.0389, wRref (F2 ) = 0.0888, T = 296(2) K.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::430befb7473d7372833af0b59083213bTest
https://doi.org/10.1515/ncrs-2018-0107Test

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